Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3268 and PP_3269 are separated by 187 nucleotides PP_3269 and PP_5596 are separated by 164 nucleotides PP_5596 and PP_5597 are separated by 78 nucleotides PP_5597 and PP_3270 are separated by 251 nucleotides
PP_3268: PP_3268 - MutT/nudix family protein, at 3,702,169 to 3,702,567
_3268
PP_3269: PP_3269 - conserved protein of unknown function, at 3,702,755 to 3,703,012
_3269
PP_5596: PP_5596 - conserved protein of unknown function, at 3,703,177 to 3,703,446
_5596
PP_5597: PP_5597 - conserved protein of unknown function, at 3,703,525 to 3,703,695
_5597
PP_3270: PP_3270 - oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, at 3,703,947 to 3,706,001
_3270
Position (kb)
3703
3704 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3702.621 kb on + strand at 3702.621 kb on + strand at 3702.686 kb on + strand at 3702.750 kb on + strand at 3702.750 kb on + strand at 3702.751 kb on - strand at 3702.751 kb on - strand at 3702.751 kb on - strand at 3702.863 kb on - strand, within PP_3269 at 3703.042 kb on + strand at 3703.042 kb on + strand at 3703.042 kb on + strand at 3703.043 kb on - strand at 3703.043 kb on - strand at 3703.043 kb on - strand at 3703.182 kb on + strand at 3703.182 kb on + strand at 3703.182 kb on + strand at 3703.195 kb on + strand at 3703.195 kb on + strand at 3703.195 kb on + strand at 3703.196 kb on - strand at 3703.196 kb on - strand at 3703.196 kb on - strand at 3703.196 kb on - strand at 3703.208 kb on - strand, within PP_5596 at 3703.507 kb on - strand at 3703.507 kb on - strand at 3703.520 kb on + strand at 3703.521 kb on - strand at 3703.521 kb on - strand at 3703.521 kb on - strand at 3703.521 kb on - strand at 3703.590 kb on + strand, within PP_5597 at 3703.590 kb on + strand, within PP_5597 at 3703.591 kb on - strand, within PP_5597 at 3703.720 kb on + strand at 3703.720 kb on + strand at 3703.720 kb on + strand at 3703.728 kb on - strand at 3703.728 kb on - strand at 3703.818 kb on + strand at 3703.818 kb on + strand at 3703.818 kb on + strand at 3703.818 kb on + strand at 3703.819 kb on - strand at 3703.819 kb on - strand at 3703.850 kb on + strand at 3703.880 kb on + strand at 3703.941 kb on + strand at 3703.941 kb on + strand at 3703.941 kb on + strand at 3703.941 kb on + strand at 3703.942 kb on - strand at 3703.942 kb on - strand at 3703.984 kb on + strand at 3703.984 kb on + strand at 3703.984 kb on + strand at 3703.985 kb on - strand at 3703.985 kb on - strand at 3704.161 kb on + strand, within PP_3270 at 3704.161 kb on + strand, within PP_3270 at 3704.162 kb on - strand, within PP_3270 at 3704.162 kb on - strand, within PP_3270 at 3704.220 kb on + strand at 3704.221 kb on - strand, within PP_3270 at 3704.228 kb on - strand, within PP_3270 at 3704.247 kb on - strand, within PP_3270 at 3704.393 kb on - strand, within PP_3270 at 3704.410 kb on - strand, within PP_3270 at 3704.443 kb on + strand, within PP_3270 at 3704.443 kb on + strand, within PP_3270 at 3704.484 kb on + strand, within PP_3270 at 3704.485 kb on - strand, within PP_3270 at 3704.503 kb on + strand, within PP_3270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,702,621 + +0.4 3,702,621 + -0.7 3,702,686 + +0.8 3,702,750 + +0.1 3,702,750 + -0.4 3,702,751 - +0.1 3,702,751 - +0.0 3,702,751 - -0.0 3,702,863 - PP_3269 0.42 +0.5 3,703,042 + -0.1 3,703,042 + +0.7 3,703,042 + -0.7 3,703,043 - -0.0 3,703,043 - +0.1 3,703,043 - -0.3 3,703,182 + +2.2 3,703,182 + +1.1 3,703,182 + -0.2 3,703,195 + +0.6 3,703,195 + +0.6 3,703,195 + -0.2 3,703,196 - +0.3 3,703,196 - +1.7 3,703,196 - +0.4 3,703,196 - -0.5 3,703,208 - PP_5596 0.11 +0.4 3,703,507 - -0.6 3,703,507 - -0.7 3,703,520 + -1.3 3,703,521 - -0.1 3,703,521 - +0.3 3,703,521 - -0.3 3,703,521 - -0.4 3,703,590 + PP_5597 0.38 +0.3 3,703,590 + PP_5597 0.38 -0.2 3,703,591 - PP_5597 0.39 +1.2 3,703,720 + +1.0 3,703,720 + +0.4 3,703,720 + +0.0 3,703,728 - -0.3 3,703,728 - +1.0 3,703,818 + -1.0 3,703,818 + -1.2 3,703,818 + +1.1 3,703,818 + -0.4 3,703,819 - -0.7 3,703,819 - +0.5 3,703,850 + -0.2 3,703,880 + +0.1 3,703,941 + -0.6 3,703,941 + +0.1 3,703,941 + -0.2 3,703,941 + +0.5 3,703,942 - +0.6 3,703,942 - +0.1 3,703,984 + -0.1 3,703,984 + +0.5 3,703,984 + -0.7 3,703,985 - -0.9 3,703,985 - -0.0 3,704,161 + PP_3270 0.10 +0.2 3,704,161 + PP_3270 0.10 +0.6 3,704,162 - PP_3270 0.10 +0.5 3,704,162 - PP_3270 0.10 -1.3 3,704,220 + -0.7 3,704,221 - PP_3270 0.13 -2.9 3,704,228 - PP_3270 0.14 +1.5 3,704,247 - PP_3270 0.15 +0.6 3,704,393 - PP_3270 0.22 -0.2 3,704,410 - PP_3270 0.23 -0.1 3,704,443 + PP_3270 0.24 -1.0 3,704,443 + PP_3270 0.24 +0.1 3,704,484 + PP_3270 0.26 -0.9 3,704,485 - PP_3270 0.26 -0.3 3,704,503 + PP_3270 0.27 -0.3
Or see this region's nucleotide sequence