Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5488 and PP_5489 are separated by 16 nucleotides PP_5489 and PP_5490 are separated by 173 nucleotides PP_5490 and PP_1936 are separated by 162 nucleotides
PP_5488: PP_5488 - conserved protein of unknown function, at 2,182,596 to 2,183,255
_5488
PP_5489: PP_5489 - conserved protein of unknown function, at 2,183,272 to 2,183,769
_5489
PP_5490: PP_5490 - site-specific DNA recombinase, at 2,183,943 to 2,184,509
_5490
PP_1936: PP_1936 - conserved membrane protein of unknown function, at 2,184,672 to 2,187,608
_1936
Position (kb)
2183
2184
2185 Strain fitness (log2 ratio)
-1
0
1
2 at 2183.189 kb on + strand, within PP_5488 at 2183.191 kb on + strand at 2183.192 kb on - strand at 2183.192 kb on - strand at 2183.195 kb on + strand at 2183.253 kb on + strand at 2183.254 kb on - strand at 2183.258 kb on + strand at 2183.259 kb on - strand at 2183.507 kb on - strand, within PP_5489 at 2183.535 kb on + strand, within PP_5489 at 2183.536 kb on - strand, within PP_5489 at 2183.592 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.743 kb on - strand at 2183.789 kb on - strand at 2183.828 kb on - strand at 2183.942 kb on + strand at 2183.992 kb on + strand at 2184.004 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.168 kb on - strand, within PP_5490 at 2184.236 kb on + strand, within PP_5490 at 2184.237 kb on - strand, within PP_5490 at 2184.256 kb on + strand, within PP_5490 at 2184.257 kb on - strand, within PP_5490 at 2184.265 kb on + strand, within PP_5490 at 2184.350 kb on - strand, within PP_5490 at 2184.377 kb on + strand, within PP_5490 at 2184.412 kb on + strand, within PP_5490 at 2184.567 kb on + strand at 2184.567 kb on + strand at 2184.568 kb on - strand at 2184.568 kb on - strand at 2184.628 kb on + strand at 2184.628 kb on + strand at 2184.628 kb on + strand at 2184.628 kb on + strand at 2184.658 kb on - strand at 2184.739 kb on + strand at 2184.739 kb on + strand at 2184.751 kb on + strand at 2184.752 kb on - strand at 2184.752 kb on - strand at 2184.763 kb on - strand at 2184.911 kb on - strand at 2185.054 kb on + strand, within PP_1936 at 2185.109 kb on - strand, within PP_1936 at 2185.114 kb on - strand, within PP_1936 at 2185.146 kb on + strand, within PP_1936 at 2185.158 kb on - strand, within PP_1936 at 2185.158 kb on - strand, within PP_1936 at 2185.449 kb on + strand, within PP_1936
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,183,189 + PP_5488 0.90 +0.5 2,183,191 + +0.8 2,183,192 - +1.0 2,183,192 - -1.0 2,183,195 + -1.2 2,183,253 + -0.3 2,183,254 - -0.4 2,183,258 + +0.7 2,183,259 - +1.4 2,183,507 - PP_5489 0.47 -0.4 2,183,535 + PP_5489 0.53 +0.8 2,183,536 - PP_5489 0.53 +0.3 2,183,592 + PP_5489 0.64 -0.3 2,183,597 + PP_5489 0.65 -0.1 2,183,597 + PP_5489 0.65 -0.1 2,183,743 - +0.3 2,183,789 - -0.0 2,183,828 - +0.2 2,183,942 + -0.2 2,183,992 + +1.3 2,184,004 + PP_5490 0.11 +1.8 2,184,167 + PP_5490 0.40 +0.8 2,184,167 + PP_5490 0.40 +0.1 2,184,168 - PP_5490 0.40 +0.7 2,184,236 + PP_5490 0.52 +0.2 2,184,237 - PP_5490 0.52 +0.8 2,184,256 + PP_5490 0.55 +0.9 2,184,257 - PP_5490 0.55 +0.0 2,184,265 + PP_5490 0.57 -0.1 2,184,350 - PP_5490 0.72 +0.3 2,184,377 + PP_5490 0.77 +0.0 2,184,412 + PP_5490 0.83 -0.1 2,184,567 + -0.4 2,184,567 + +0.6 2,184,568 - -0.2 2,184,568 - -0.7 2,184,628 + +0.8 2,184,628 + +0.1 2,184,628 + -0.0 2,184,628 + -0.2 2,184,658 - -0.0 2,184,739 + +0.2 2,184,739 + +0.5 2,184,751 + +1.0 2,184,752 - -0.5 2,184,752 - +0.1 2,184,763 - +0.2 2,184,911 - +0.4 2,185,054 + PP_1936 0.13 -0.4 2,185,109 - PP_1936 0.15 -0.8 2,185,114 - PP_1936 0.15 +0.3 2,185,146 + PP_1936 0.16 -0.8 2,185,158 - PP_1936 0.17 -0.0 2,185,158 - PP_1936 0.17 +0.2 2,185,449 + PP_1936 0.26 +1.1
Or see this region's nucleotide sequence