Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0638 and PP_5448 are separated by 793 nucleotides PP_5448 and PP_5449 are separated by 65 nucleotides
PP_0638: PP_0638 - transposase, at 746,517 to 746,873
_0638
PP_5448: PP_5448 - conserved protein of unknown function, at 747,667 to 747,882
_5448
PP_5449: PP_5449 - conserved exported protein of unknown function, at 747,948 to 748,766
_5449
Position (kb) 747
748 Strain fitness (log2 ratio) -1
0
1 at 746.965 kb on + strand at 746.965 kb on + strand at 746.984 kb on + strand at 746.984 kb on + strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 746.985 kb on - strand at 747.037 kb on + strand at 747.037 kb on + strand at 747.038 kb on - strand at 747.063 kb on + strand at 747.064 kb on - strand at 747.064 kb on - strand at 747.168 kb on + strand at 747.230 kb on - strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.259 kb on + strand at 747.268 kb on - strand at 747.305 kb on - strand at 747.305 kb on - strand at 747.367 kb on + strand at 747.368 kb on - strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.405 kb on + strand at 747.406 kb on - strand at 747.497 kb on + strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.498 kb on - strand at 747.511 kb on + strand at 747.511 kb on + strand at 747.512 kb on - strand at 747.683 kb on + strand at 747.720 kb on + strand, within PP_5448 at 747.720 kb on + strand, within PP_5448 at 747.721 kb on - strand, within PP_5448 at 747.787 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.792 kb on + strand, within PP_5448 at 747.886 kb on + strand at 747.954 kb on - strand at 747.977 kb on - strand at 748.034 kb on + strand, within PP_5449 at 748.034 kb on + strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.035 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.039 kb on - strand, within PP_5449 at 748.110 kb on - strand, within PP_5449 at 748.127 kb on + strand, within PP_5449 at 748.253 kb on + strand, within PP_5449 at 748.312 kb on - strand, within PP_5449 at 748.328 kb on - strand, within PP_5449 at 748.494 kb on - strand, within PP_5449 at 748.566 kb on + strand, within PP_5449 at 748.738 kb on - strand at 748.773 kb on - strand at 748.812 kb on + strand at 748.812 kb on + strand at 748.879 kb on + strand at 748.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 746,965 + +0.1 746,965 + +0.2 746,984 + +0.9 746,984 + -0.7 746,985 - +0.1 746,985 - -0.1 746,985 - +1.0 746,985 - +1.0 746,985 - +0.5 746,985 - +0.3 746,985 - +0.6 747,037 + +0.3 747,037 + -0.3 747,038 - +0.2 747,063 + +1.0 747,064 - +0.6 747,064 - -0.8 747,168 + +0.0 747,230 - -1.1 747,259 + -0.5 747,259 + +0.1 747,259 + -0.5 747,268 - +0.8 747,305 - +0.3 747,305 - -0.3 747,367 + +0.4 747,368 - +0.2 747,405 + -0.8 747,405 + -0.2 747,405 + -0.3 747,406 - +0.2 747,497 + +0.2 747,498 - -0.9 747,498 - -0.7 747,498 - +1.1 747,498 - -0.2 747,511 + +0.4 747,511 + +0.5 747,512 - +0.4 747,683 + +1.1 747,720 + PP_5448 0.25 -0.1 747,720 + PP_5448 0.25 +0.2 747,721 - PP_5448 0.25 +0.5 747,787 + PP_5448 0.56 +1.7 747,792 + PP_5448 0.58 -0.0 747,792 + PP_5448 0.58 -0.2 747,886 + -0.7 747,954 - +0.7 747,977 - +0.8 748,034 + PP_5449 0.11 +0.2 748,034 + PP_5449 0.11 -0.5 748,035 - PP_5449 0.11 -0.3 748,035 - PP_5449 0.11 +0.7 748,039 - PP_5449 0.11 +0.3 748,039 - PP_5449 0.11 +0.0 748,110 - PP_5449 0.20 -0.0 748,127 + PP_5449 0.22 +0.2 748,253 + PP_5449 0.37 -0.1 748,312 - PP_5449 0.44 -0.1 748,328 - PP_5449 0.46 +0.0 748,494 - PP_5449 0.67 +0.1 748,566 + PP_5449 0.75 +1.2 748,738 - -0.8 748,773 - +0.1 748,812 + +1.1 748,812 + +0.3 748,879 + -0.1 748,880 - +0.5
Or see this region's nucleotide sequence