Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3322 and PP_3323 are separated by 46 nucleotides PP_3323 and PP_3324 are separated by 20 nucleotides
PP_3322: PP_3322 - porphobilinogen synthase (delta-aminolevulinic acid dehydratase), at 3,757,688 to 3,758,689
_3322
PP_3323: PP_3323 - CobW/P47K family protein, at 3,758,736 to 3,759,347
_3323
PP_3324: PP_3324 - GTP cyclohydrolase I, at 3,759,368 to 3,760,342
_3324
Position (kb)
3758
3759
3760 Strain fitness (log2 ratio)
-2
-1
0
1 at 3757.881 kb on + strand, within PP_3322 at 3757.882 kb on - strand, within PP_3322 at 3757.914 kb on + strand, within PP_3322 at 3757.915 kb on - strand, within PP_3322 at 3758.096 kb on + strand, within PP_3322 at 3758.097 kb on - strand, within PP_3322 at 3758.097 kb on - strand, within PP_3322 at 3758.097 kb on - strand, within PP_3322 at 3758.163 kb on + strand, within PP_3322 at 3758.163 kb on + strand, within PP_3322 at 3758.163 kb on + strand at 3758.163 kb on + strand, within PP_3322 at 3758.300 kb on + strand, within PP_3322 at 3758.300 kb on + strand, within PP_3322 at 3758.313 kb on - strand, within PP_3322 at 3758.328 kb on - strand, within PP_3322 at 3758.328 kb on - strand, within PP_3322 at 3758.387 kb on + strand, within PP_3322 at 3758.387 kb on + strand, within PP_3322 at 3758.388 kb on - strand, within PP_3322 at 3758.431 kb on - strand, within PP_3322 at 3758.487 kb on - strand, within PP_3322 at 3758.487 kb on - strand, within PP_3322 at 3758.558 kb on + strand, within PP_3322 at 3758.648 kb on - strand at 3758.662 kb on + strand at 3758.663 kb on - strand at 3758.757 kb on - strand at 3758.757 kb on - strand at 3758.819 kb on + strand, within PP_3323 at 3758.819 kb on + strand, within PP_3323 at 3758.914 kb on + strand, within PP_3323 at 3758.914 kb on + strand at 3759.025 kb on + strand, within PP_3323 at 3759.128 kb on + strand, within PP_3323 at 3759.129 kb on - strand, within PP_3323 at 3759.198 kb on + strand, within PP_3323 at 3759.199 kb on - strand, within PP_3323 at 3759.199 kb on - strand, within PP_3323 at 3759.390 kb on + strand at 3759.390 kb on + strand at 3759.391 kb on - strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.392 kb on + strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.393 kb on - strand at 3759.512 kb on + strand, within PP_3324 at 3759.513 kb on - strand, within PP_3324 at 3759.997 kb on + strand, within PP_3324 at 3759.997 kb on + strand, within PP_3324 at 3759.998 kb on - strand, within PP_3324 at 3759.998 kb on - strand, within PP_3324 at 3759.998 kb on - strand, within PP_3324 at 3759.998 kb on - strand, within PP_3324 at 3759.998 kb on - strand, within PP_3324 at 3760.032 kb on + strand, within PP_3324 at 3760.193 kb on - strand, within PP_3324
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,757,881 + PP_3322 0.19 +0.7 3,757,882 - PP_3322 0.19 +0.2 3,757,914 + PP_3322 0.23 -0.3 3,757,915 - PP_3322 0.23 +0.1 3,758,096 + PP_3322 0.41 +0.3 3,758,097 - PP_3322 0.41 -0.5 3,758,097 - PP_3322 0.41 +0.7 3,758,097 - PP_3322 0.41 +0.2 3,758,163 + PP_3322 0.47 -1.8 3,758,163 + PP_3322 0.47 -0.0 3,758,163 + +0.8 3,758,163 + PP_3322 0.47 -0.0 3,758,300 + PP_3322 0.61 +0.5 3,758,300 + PP_3322 0.61 -0.4 3,758,313 - PP_3322 0.62 +0.0 3,758,328 - PP_3322 0.64 +0.6 3,758,328 - PP_3322 0.64 +0.6 3,758,387 + PP_3322 0.70 +0.7 3,758,387 + PP_3322 0.70 -0.1 3,758,388 - PP_3322 0.70 +0.9 3,758,431 - PP_3322 0.74 +1.1 3,758,487 - PP_3322 0.80 -0.6 3,758,487 - PP_3322 0.80 +0.4 3,758,558 + PP_3322 0.87 +0.2 3,758,648 - +0.2 3,758,662 + -1.4 3,758,663 - +0.5 3,758,757 - +1.7 3,758,757 - -0.2 3,758,819 + PP_3323 0.14 -0.7 3,758,819 + PP_3323 0.14 +0.1 3,758,914 + PP_3323 0.29 -0.4 3,758,914 + +1.0 3,759,025 + PP_3323 0.47 +1.4 3,759,128 + PP_3323 0.64 +0.8 3,759,129 - PP_3323 0.64 +1.2 3,759,198 + PP_3323 0.75 +0.1 3,759,199 - PP_3323 0.76 +1.2 3,759,199 - PP_3323 0.76 -0.2 3,759,390 + -0.3 3,759,390 + -0.6 3,759,391 - +0.3 3,759,392 + +0.6 3,759,392 + +0.7 3,759,392 + +0.5 3,759,392 + +0.9 3,759,392 + -0.8 3,759,392 + -0.2 3,759,392 + +1.4 3,759,392 + -0.3 3,759,393 - -0.4 3,759,393 - -0.6 3,759,393 - -0.6 3,759,393 - +0.1 3,759,393 - +1.6 3,759,393 - +0.4 3,759,393 - -0.0 3,759,512 + PP_3324 0.15 -0.5 3,759,513 - PP_3324 0.15 +1.0 3,759,997 + PP_3324 0.65 +0.6 3,759,997 + PP_3324 0.65 +0.4 3,759,998 - PP_3324 0.65 -1.5 3,759,998 - PP_3324 0.65 +0.1 3,759,998 - PP_3324 0.65 +0.6 3,759,998 - PP_3324 0.65 -1.1 3,759,998 - PP_3324 0.65 +0.5 3,760,032 + PP_3324 0.68 -0.1 3,760,193 - PP_3324 0.85 -0.8
Or see this region's nucleotide sequence