Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2887 and PP_2888 are separated by 124 nucleotides PP_2888 and PP_2889 overlap by 4 nucleotides PP_2889 and PP_2890 are separated by 40 nucleotides PP_2890 and PP_2891 are separated by 135 nucleotides
PP_2887: PP_2887 - Catalase-related peroxidase, at 3,284,812 to 3,285,864
_2887
PP_2888: PP_2888 - putative ECF sigma factor PrtI, at 3,285,989 to 3,286,510
_2888
PP_2889: PP_2889 - putative Transmembrane regulator PrtR, at 3,286,507 to 3,287,259
_2889
PP_2890: PP_2890 - exodeoxyribonuclease III / apurinic/apyrimidinic endodeoxyribonuclease VI, at 3,287,300 to 3,288,112
_2890
PP_2891: PP_2891 - Acetyltransferase, GNAT family, at 3,288,248 to 3,288,925
_2891
Position (kb)
3286
3287
3288 Strain fitness (log2 ratio)
-2
-1
0
1 at 3285.522 kb on + strand, within PP_2887 at 3285.523 kb on - strand, within PP_2887 at 3285.523 kb on - strand, within PP_2887 at 3285.523 kb on - strand, within PP_2887 at 3285.645 kb on - strand, within PP_2887 at 3285.889 kb on + strand at 3285.956 kb on + strand at 3285.985 kb on - strand at 3286.096 kb on + strand, within PP_2888 at 3287.048 kb on + strand, within PP_2889 at 3287.049 kb on - strand, within PP_2889 at 3287.054 kb on + strand, within PP_2889 at 3287.165 kb on - strand, within PP_2889 at 3287.182 kb on + strand, within PP_2889 at 3287.182 kb on + strand, within PP_2889 at 3287.183 kb on - strand, within PP_2889 at 3287.183 kb on - strand, within PP_2889 at 3287.184 kb on + strand at 3287.257 kb on + strand at 3287.257 kb on + strand at 3287.257 kb on + strand at 3287.257 kb on + strand at 3287.257 kb on + strand at 3287.258 kb on - strand at 3287.298 kb on - strand at 3287.361 kb on + strand at 3287.361 kb on + strand at 3287.503 kb on - strand, within PP_2890 at 3287.523 kb on - strand, within PP_2890 at 3287.523 kb on - strand, within PP_2890 at 3287.523 kb on - strand, within PP_2890 at 3287.784 kb on + strand, within PP_2890 at 3287.831 kb on - strand, within PP_2890 at 3287.946 kb on + strand, within PP_2890 at 3287.946 kb on + strand, within PP_2890 at 3287.947 kb on - strand, within PP_2890 at 3287.947 kb on - strand, within PP_2890 at 3287.958 kb on + strand, within PP_2890 at 3288.195 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 3,285,522 + PP_2887 0.67 +0.4 3,285,523 - PP_2887 0.68 -0.7 3,285,523 - PP_2887 0.68 -1.8 3,285,523 - PP_2887 0.68 -0.1 3,285,645 - PP_2887 0.79 +0.5 3,285,889 + -0.0 3,285,956 + +0.0 3,285,985 - -0.7 3,286,096 + PP_2888 0.20 +0.8 3,287,048 + PP_2889 0.72 +1.2 3,287,049 - PP_2889 0.72 +0.6 3,287,054 + PP_2889 0.73 +0.7 3,287,165 - PP_2889 0.87 -1.0 3,287,182 + PP_2889 0.90 +1.2 3,287,182 + PP_2889 0.90 +0.6 3,287,183 - PP_2889 0.90 +0.7 3,287,183 - PP_2889 0.90 +0.2 3,287,184 + +0.6 3,287,257 + +0.2 3,287,257 + +0.3 3,287,257 + -1.0 3,287,257 + +0.7 3,287,257 + +0.2 3,287,258 - -1.0 3,287,298 - -0.5 3,287,361 + +1.2 3,287,361 + -1.0 3,287,503 - PP_2890 0.25 +0.1 3,287,523 - PP_2890 0.27 +0.6 3,287,523 - PP_2890 0.27 -1.2 3,287,523 - PP_2890 0.27 -0.4 3,287,784 + PP_2890 0.60 -0.2 3,287,831 - PP_2890 0.65 +0.2 3,287,946 + PP_2890 0.79 +0.5 3,287,946 + PP_2890 0.79 -0.8 3,287,947 - PP_2890 0.80 -0.3 3,287,947 - PP_2890 0.80 -0.7 3,287,958 + PP_2890 0.81 +0.2 3,288,195 + +0.2
Or see this region's nucleotide sequence