Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2601 and PP_2602 are separated by 138 nucleotides PP_2602 and PP_2603 are separated by 29 nucleotides PP_2603 and PP_2604 are separated by 148 nucleotides
PP_2601: PP_2601 - Transcriptional regulator, IclR family, at 2,974,068 to 2,974,844
_2601
PP_2602: PP_2602 - putative 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, at 2,974,983 to 2,976,035
_2602
PP_2603: PP_2603 - conserved protein of unknown function, at 2,976,065 to 2,976,880
_2603
PP_2604: PP_2604 - major facilitator family transporter, at 2,977,029 to 2,978,354
_2604
Position (kb)
2974
2975
2976
2977 Strain fitness (log2 ratio)
-1
0
1 at 2974.105 kb on + strand at 2974.106 kb on - strand at 2974.106 kb on - strand at 2974.141 kb on + strand at 2974.141 kb on + strand at 2974.218 kb on + strand, within PP_2601 at 2974.218 kb on + strand, within PP_2601 at 2974.218 kb on + strand, within PP_2601 at 2974.219 kb on - strand, within PP_2601 at 2974.248 kb on + strand, within PP_2601 at 2974.270 kb on + strand, within PP_2601 at 2974.288 kb on - strand, within PP_2601 at 2974.470 kb on + strand, within PP_2601 at 2974.470 kb on + strand, within PP_2601 at 2974.470 kb on + strand, within PP_2601 at 2974.471 kb on - strand, within PP_2601 at 2974.471 kb on - strand, within PP_2601 at 2974.471 kb on - strand, within PP_2601 at 2974.544 kb on + strand, within PP_2601 at 2974.605 kb on + strand, within PP_2601 at 2974.605 kb on + strand, within PP_2601 at 2974.783 kb on + strand at 2975.001 kb on - strand at 2975.001 kb on - strand at 2975.329 kb on + strand, within PP_2602 at 2975.341 kb on + strand, within PP_2602 at 2975.342 kb on - strand, within PP_2602 at 2975.460 kb on + strand, within PP_2602 at 2975.460 kb on + strand, within PP_2602 at 2975.461 kb on - strand, within PP_2602 at 2975.461 kb on - strand, within PP_2602 at 2975.541 kb on + strand, within PP_2602 at 2975.752 kb on - strand, within PP_2602 at 2975.835 kb on - strand, within PP_2602 at 2975.983 kb on + strand at 2976.036 kb on + strand at 2976.037 kb on - strand at 2976.037 kb on - strand at 2976.044 kb on + strand at 2976.044 kb on + strand at 2976.045 kb on - strand at 2976.045 kb on - strand at 2976.045 kb on - strand at 2976.047 kb on + strand at 2976.047 kb on + strand at 2976.048 kb on - strand at 2976.048 kb on - strand at 2976.062 kb on + strand at 2976.062 kb on + strand at 2976.063 kb on - strand at 2976.153 kb on - strand, within PP_2603 at 2976.376 kb on - strand, within PP_2603 at 2976.447 kb on - strand, within PP_2603 at 2976.489 kb on + strand, within PP_2603 at 2976.490 kb on - strand, within PP_2603 at 2976.490 kb on - strand, within PP_2603 at 2976.490 kb on - strand, within PP_2603 at 2976.629 kb on + strand, within PP_2603 at 2976.629 kb on + strand, within PP_2603 at 2976.748 kb on - strand, within PP_2603 at 2977.013 kb on + strand at 2977.013 kb on + strand at 2977.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 2,974,105 + -0.4 2,974,106 - +0.4 2,974,106 - -0.5 2,974,141 + +0.0 2,974,141 + -1.2 2,974,218 + PP_2601 0.19 +0.1 2,974,218 + PP_2601 0.19 +0.4 2,974,218 + PP_2601 0.19 -1.2 2,974,219 - PP_2601 0.19 +0.3 2,974,248 + PP_2601 0.23 -1.6 2,974,270 + PP_2601 0.26 +0.5 2,974,288 - PP_2601 0.28 -0.1 2,974,470 + PP_2601 0.52 +1.1 2,974,470 + PP_2601 0.52 -0.7 2,974,470 + PP_2601 0.52 +0.7 2,974,471 - PP_2601 0.52 +0.3 2,974,471 - PP_2601 0.52 +0.6 2,974,471 - PP_2601 0.52 +0.0 2,974,544 + PP_2601 0.61 -0.0 2,974,605 + PP_2601 0.69 -0.8 2,974,605 + PP_2601 0.69 -0.3 2,974,783 + -1.3 2,975,001 - +0.1 2,975,001 - -0.2 2,975,329 + PP_2602 0.33 +0.8 2,975,341 + PP_2602 0.34 +0.0 2,975,342 - PP_2602 0.34 +1.1 2,975,460 + PP_2602 0.45 +0.3 2,975,460 + PP_2602 0.45 -0.6 2,975,461 - PP_2602 0.45 +0.4 2,975,461 - PP_2602 0.45 +1.1 2,975,541 + PP_2602 0.53 +0.1 2,975,752 - PP_2602 0.73 +1.0 2,975,835 - PP_2602 0.81 +0.9 2,975,983 + +0.4 2,976,036 + +0.2 2,976,037 - +0.8 2,976,037 - -0.3 2,976,044 + -0.0 2,976,044 + -0.0 2,976,045 - -0.5 2,976,045 - -0.0 2,976,045 - +0.3 2,976,047 + +0.3 2,976,047 + -0.4 2,976,048 - -0.3 2,976,048 - +0.6 2,976,062 + -1.2 2,976,062 + +1.1 2,976,063 - +0.8 2,976,153 - PP_2603 0.11 +0.3 2,976,376 - PP_2603 0.38 +0.5 2,976,447 - PP_2603 0.47 -0.2 2,976,489 + PP_2603 0.52 +0.4 2,976,490 - PP_2603 0.52 +0.1 2,976,490 - PP_2603 0.52 -0.1 2,976,490 - PP_2603 0.52 -0.1 2,976,629 + PP_2603 0.69 -0.9 2,976,629 + PP_2603 0.69 -0.7 2,976,748 - PP_2603 0.84 +0.3 2,977,013 + -1.1 2,977,013 + +1.2 2,977,014 - +0.0
Or see this region's nucleotide sequence