Experiment: R2A with Fusaric 120 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1003 and PP_1004 overlap by 21 nucleotides PP_1004 and PP_1005 are separated by 54 nucleotides PP_1005 and PP_1006 are separated by 140 nucleotides
PP_1003: PP_1003 - arginine/ornithine antiporter, at 1,142,865 to 1,144,292
_1003
PP_1004: PP_1004 - conserved protein of unknown function, at 1,144,272 to 1,144,685
_1004
PP_1005: PP_1005 - Heme oxygenase, at 1,144,740 to 1,145,333
_1005
PP_1006: PP_1006 - putative outer membrane heme receptor, at 1,145,474 to 1,148,062
_1006
Position (kb)
1144
1145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1143.281 kb on - strand, within PP_1003 at 1143.905 kb on + strand, within PP_1003 at 1143.906 kb on - strand, within PP_1003 at 1143.923 kb on + strand, within PP_1003 at 1143.927 kb on + strand, within PP_1003 at 1143.927 kb on + strand, within PP_1003 at 1143.927 kb on + strand, within PP_1003 at 1143.927 kb on + strand, within PP_1003 at 1143.928 kb on - strand, within PP_1003 at 1143.928 kb on - strand, within PP_1003 at 1143.972 kb on + strand, within PP_1003 at 1143.973 kb on - strand, within PP_1003 at 1143.973 kb on - strand, within PP_1003 at 1143.991 kb on - strand, within PP_1003 at 1144.084 kb on - strand, within PP_1003 at 1144.101 kb on + strand, within PP_1003 at 1144.101 kb on + strand, within PP_1003 at 1144.102 kb on - strand, within PP_1003 at 1144.143 kb on + strand, within PP_1003 at 1144.144 kb on - strand, within PP_1003 at 1144.260 kb on + strand at 1144.261 kb on - strand at 1144.269 kb on + strand at 1144.478 kb on + strand, within PP_1004 at 1144.479 kb on - strand, within PP_1004 at 1144.630 kb on + strand, within PP_1004 at 1144.631 kb on - strand, within PP_1004 at 1144.631 kb on - strand, within PP_1004 at 1144.677 kb on + strand at 1144.698 kb on + strand at 1144.698 kb on + strand at 1144.698 kb on + strand at 1144.699 kb on - strand at 1144.699 kb on - strand at 1144.699 kb on - strand at 1144.765 kb on + strand at 1145.190 kb on + strand, within PP_1005 at 1145.190 kb on + strand, within PP_1005 at 1145.191 kb on - strand, within PP_1005 at 1145.191 kb on - strand, within PP_1005 at 1145.191 kb on - strand, within PP_1005 at 1145.191 kb on - strand, within PP_1005 at 1145.191 kb on - strand, within PP_1005 at 1145.194 kb on + strand, within PP_1005 at 1145.195 kb on - strand, within PP_1005 at 1145.195 kb on - strand, within PP_1005 at 1145.361 kb on - strand at 1145.364 kb on + strand at 1145.365 kb on - strand at 1145.365 kb on - strand at 1145.365 kb on - strand at 1145.407 kb on - strand at 1145.407 kb on - strand at 1145.467 kb on + strand at 1145.498 kb on - strand at 1145.498 kb on - strand at 1145.575 kb on - strand at 1145.602 kb on - strand at 1145.602 kb on - strand at 1145.622 kb on + strand at 1145.623 kb on - strand at 1145.623 kb on - strand at 1145.623 kb on - strand at 1145.623 kb on - strand at 1145.623 kb on - strand at 1145.661 kb on + strand at 1145.661 kb on + strand at 1145.661 kb on + strand at 1145.661 kb on + strand at 1145.661 kb on + strand at 1145.662 kb on - strand at 1145.662 kb on - strand at 1145.662 kb on - strand at 1145.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusaric 120 ug/mL remove 1,143,281 - PP_1003 0.29 -0.2 1,143,905 + PP_1003 0.73 -0.9 1,143,906 - PP_1003 0.73 -2.2 1,143,923 + PP_1003 0.74 -0.2 1,143,927 + PP_1003 0.74 -0.5 1,143,927 + PP_1003 0.74 -3.4 1,143,927 + PP_1003 0.74 -0.8 1,143,927 + PP_1003 0.74 +1.2 1,143,928 - PP_1003 0.74 -0.0 1,143,928 - PP_1003 0.74 +0.3 1,143,972 + PP_1003 0.78 +0.3 1,143,973 - PP_1003 0.78 +0.0 1,143,973 - PP_1003 0.78 +0.2 1,143,991 - PP_1003 0.79 -0.3 1,144,084 - PP_1003 0.85 +0.5 1,144,101 + PP_1003 0.87 -0.2 1,144,101 + PP_1003 0.87 -0.3 1,144,102 - PP_1003 0.87 -0.1 1,144,143 + PP_1003 0.89 -0.1 1,144,144 - PP_1003 0.90 -0.3 1,144,260 + -0.1 1,144,261 - +1.0 1,144,269 + -0.5 1,144,478 + PP_1004 0.50 +1.1 1,144,479 - PP_1004 0.50 +0.7 1,144,630 + PP_1004 0.86 -0.6 1,144,631 - PP_1004 0.87 +0.8 1,144,631 - PP_1004 0.87 +1.3 1,144,677 + +0.8 1,144,698 + -0.1 1,144,698 + +0.7 1,144,698 + -0.4 1,144,699 - -0.6 1,144,699 - -0.8 1,144,699 - +0.3 1,144,765 + -1.1 1,145,190 + PP_1005 0.76 -0.3 1,145,190 + PP_1005 0.76 +0.5 1,145,191 - PP_1005 0.76 -1.9 1,145,191 - PP_1005 0.76 +0.2 1,145,191 - PP_1005 0.76 -0.2 1,145,191 - PP_1005 0.76 -0.9 1,145,191 - PP_1005 0.76 -0.8 1,145,194 + PP_1005 0.76 +0.0 1,145,195 - PP_1005 0.77 -1.1 1,145,195 - PP_1005 0.77 +0.1 1,145,361 - -0.1 1,145,364 + +1.0 1,145,365 - +0.1 1,145,365 - +2.1 1,145,365 - -0.3 1,145,407 - +0.9 1,145,407 - -2.1 1,145,467 + -0.1 1,145,498 - -1.2 1,145,498 - +0.6 1,145,575 - +0.1 1,145,602 - +0.7 1,145,602 - -0.5 1,145,622 + -0.1 1,145,623 - -0.6 1,145,623 - -1.0 1,145,623 - +0.9 1,145,623 - -0.2 1,145,623 - +0.0 1,145,661 + -1.0 1,145,661 + -0.0 1,145,661 + -0.9 1,145,661 + +0.5 1,145,661 + -0.8 1,145,662 - -0.3 1,145,662 - -0.9 1,145,662 - -0.3 1,145,662 - -0.1
Or see this region's nucleotide sequence