Strain Fitness in Pseudomonas putida KT2440 around PP_5732

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
6,130,581	+		PP_5379	0.13	-0.6
6,130,581	+		PP_5379	0.13	-2.0
6,130,582	-		PP_5379	0.13	-0.5
6,130,586	+		PP_5379	0.13	-0.2
6,130,587	-		PP_5379	0.13	+0.1
6,130,660	+		PP_5379	0.20	-1.4
6,130,710	+		PP_5379	0.25	+0.1
6,130,710	+		PP_5379	0.25	-0.3
6,130,710	+		PP_5379	0.25	-1.2
6,130,711	-		PP_5379	0.25	+0.2
6,130,781	-		PP_5379	0.31	+0.3
6,130,782	+		PP_5379	0.31	+0.9
6,130,782	+		PP_5379	0.31	-0.7
6,130,783	-		PP_5379	0.32	+1.5
6,130,879	+		PP_5379	0.40	+0.1
6,130,880	-		PP_5379	0.41	-0.9
6,130,894	-		PP_5379	0.42	-0.4
6,131,032	-		PP_5379	0.55	+0.8
6,131,041	+		PP_5379	0.55	-1.4
6,131,041	+		PP_5379	0.55	+0.3
6,131,041	+		PP_5379	0.55	+0.6
6,131,041	+		PP_5379	0.55	-1.4
6,131,042	-		PP_5379	0.56	-2.5
6,131,042	-		PP_5379	0.56	-2.6
6,131,042	-		PP_5379	0.56	+0.8
6,131,042	-		PP_5379	0.56	+0.8
6,131,042	-		PP_5379	0.56	+0.8
6,131,042	-		PP_5379	0.56	-1.2
6,131,042	-		PP_5379	0.56	-0.7
6,131,045	+		PP_5379	0.56	-2.8
6,131,045	+		PP_5379	0.56	-2.2
6,131,045	+		PP_5379	0.56	+0.3
6,131,146	+		PP_5379	0.65	+0.6
6,131,146	+		PP_5379	0.65	+0.8
6,131,146	+		PP_5379	0.65	+0.3
6,131,152	+		PP_5379	0.66	+1.2
6,131,153	-		PP_5379	0.66	-1.6
6,131,329	+		PP_5379	0.82	-1.2
6,131,330	-		PP_5379	0.82	-2.7
6,131,330	-		PP_5379	0.82	-0.7
6,131,513	-				-0.2
6,131,534	+				+0.9
6,131,535	-				-0.0
6,131,535	-				+0.1
6,131,535	-				-0.2
6,131,540	+				+0.1
6,131,579	+		PP_5732	0.21	+1.3
6,131,579	+		PP_5732	0.21	+1.1
6,131,580	-		PP_5732	0.22	+0.6
6,131,603	+		PP_5732	0.29	-1.3
6,131,604	-		PP_5732	0.29	+0.1
6,131,604	-		PP_5732	0.29	-0.7
6,131,604	-		PP_5732	0.29	+0.3
6,131,628	-		PP_5732	0.37	+0.2
6,131,651	+		PP_5732	0.44	-2.0
6,131,652	-		PP_5732	0.44	+1.4
6,131,652	-		PP_5732	0.44	+1.2
6,131,741	+		PP_5732	0.72	+0.6
6,131,742	-		PP_5732	0.73	+0.3
6,131,761	+		PP_5732	0.79	-0.1
6,131,761	+		PP_5732	0.79	-0.8
6,131,762	-		PP_5732	0.79	-1.5
6,131,762	-		PP_5732	0.79	+1.2
6,131,884	+				-0.5
6,131,894	+				-1.6
6,131,894	+				-1.1
6,131,894	+				+0.5
6,131,895	-				-1.0
6,131,895	-				-1.0
6,131,895	-				+0.4
6,131,895	-				+0.8
6,131,895	-				+0.4
6,131,907	+				+0.7
6,131,908	-				-1.0
6,131,908	-				-0.5
6,131,943	+				+0.6
6,131,998	+				+1.1
6,131,999	-				-0.2
6,132,096	+		PP_5380	0.13	+0.0
6,132,096	+		PP_5380	0.13	-0.7
6,132,097	-		PP_5380	0.13	+0.6
6,132,107	+		PP_5380	0.13	-1.0
6,132,243	+		PP_5380	0.20	+0.5
6,132,244	-		PP_5380	0.20	-0.4
6,132,244	-		PP_5380	0.20	-0.4
6,132,244	-		PP_5380	0.20	-1.3
6,132,298	+		PP_5380	0.23	-0.4
6,132,298	+		PP_5380	0.23	+0.2
6,132,313	+		PP_5380	0.23	+0.1
6,132,313	+		PP_5380	0.23	-1.0
6,132,314	-		PP_5380	0.23	+1.0
6,132,314	-				+1.9
6,132,314	-		PP_5380	0.23	-0.2
6,132,340	+		PP_5380	0.25	+1.5
6,132,340	+		PP_5380	0.25	+0.5
6,132,340	+		PP_5380	0.25	-0.1
6,132,340	+		PP_5380	0.25	-0.3
6,132,341	-		PP_5380	0.25	+0.8
6,132,341	-		PP_5380	0.25	-1.6
6,132,341	-		PP_5380	0.25	+1.3
6,132,341	-		PP_5380	0.25	+0.8
6,132,341	-		PP_5380	0.25	+0.9
6,132,341	-		PP_5380	0.25	-0.0
6,132,379	+		PP_5380	0.27	-0.8
6,132,380	-		PP_5380	0.27	-0.7
6,132,442	+		PP_5380	0.30	+0.3
6,132,443	-		PP_5380	0.30	+1.1
6,132,443	-		PP_5380	0.30	-2.5
6,132,443	-		PP_5380	0.30	+1.4
6,132,443	-		PP_5380	0.30	-0.1
6,132,745	+		PP_5380	0.45	-0.6
6,132,799	+		PP_5380	0.48	+1.1
6,132,799	+		PP_5380	0.48	+1.5
6,132,799	+		PP_5380	0.48	-2.0
6,132,800	-		PP_5380	0.48	+0.3
6,132,800	-		PP_5380	0.48	+0.4
6,132,800	-		PP_5380	0.48	-0.6
6,132,816	-		PP_5380	0.48	-1.0