Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2939 and PP_2940 are separated by 19 nucleotides PP_2940 and PP_5541 are separated by 591 nucleotides PP_5541 and PP_5542 are separated by 74 nucleotides PP_5542 and PP_2941 are separated by 71 nucleotides
PP_2939: PP_2939 - toxin of the YefM-YoeB antitoxin/toxin complex and DNA-binding transcriptional repressor, at 3,342,448 to 3,342,702
_2939
PP_2940: PP_2940 - antitoxin of the YoeB-YefM toxin-antitoxin pair and DNA binding transcriptional repressor, at 3,342,722 to 3,343,006
_2940
PP_5541: PP_5541 - conserved protein of unknown function, at 3,343,598 to 3,343,927
_5541
PP_5542: PP_5542 - protein of unknown function, at 3,344,002 to 3,344,271
_5542
PP_2941: PP_2941 - conserved protein of unknown function, at 3,344,343 to 3,345,128
_2941
Position (kb)
3343
3344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3342.605 kb on - strand, within PP_2939 at 3342.619 kb on + strand, within PP_2939 at 3342.671 kb on + strand, within PP_2939 at 3342.671 kb on + strand, within PP_2939 at 3342.671 kb on + strand at 3342.672 kb on - strand, within PP_2939 at 3342.706 kb on + strand at 3342.706 kb on + strand at 3342.706 kb on + strand at 3342.706 kb on + strand at 3342.707 kb on - strand at 3342.707 kb on - strand at 3342.707 kb on - strand at 3343.226 kb on - strand at 3343.269 kb on + strand at 3343.269 kb on + strand at 3343.270 kb on - strand at 3343.302 kb on + strand at 3343.303 kb on - strand at 3343.338 kb on - strand at 3343.339 kb on + strand at 3343.340 kb on - strand at 3343.486 kb on + strand at 3343.486 kb on + strand at 3343.504 kb on - strand at 3343.525 kb on + strand at 3343.525 kb on + strand at 3343.555 kb on - strand at 3343.586 kb on + strand at 3343.659 kb on - strand, within PP_5541 at 3343.741 kb on + strand, within PP_5541 at 3343.741 kb on + strand, within PP_5541 at 3343.746 kb on - strand, within PP_5541 at 3343.779 kb on + strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.780 kb on - strand, within PP_5541 at 3343.970 kb on + strand at 3343.970 kb on + strand at 3343.971 kb on - strand at 3344.045 kb on - strand, within PP_5542 at 3344.045 kb on - strand, within PP_5542 at 3344.056 kb on + strand, within PP_5542 at 3344.057 kb on - strand, within PP_5542 at 3344.066 kb on - strand, within PP_5542 at 3344.133 kb on - strand, within PP_5542 at 3344.231 kb on - strand, within PP_5542 at 3344.231 kb on - strand, within PP_5542 at 3344.410 kb on + strand at 3344.411 kb on - strand at 3344.545 kb on - strand, within PP_2941 at 3344.545 kb on - strand, within PP_2941 at 3344.656 kb on + strand, within PP_2941 at 3344.656 kb on + strand, within PP_2941 at 3344.657 kb on - strand, within PP_2941 at 3344.657 kb on - strand, within PP_2941 at 3344.660 kb on - strand, within PP_2941 at 3344.660 kb on - strand, within PP_2941 at 3344.698 kb on + strand, within PP_2941 at 3344.699 kb on - strand, within PP_2941 at 3344.903 kb on - strand, within PP_2941
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,342,605 - PP_2939 0.62 +0.2 3,342,619 + PP_2939 0.67 -1.2 3,342,671 + PP_2939 0.87 +1.0 3,342,671 + PP_2939 0.87 -2.8 3,342,671 + +0.8 3,342,672 - PP_2939 0.88 -0.4 3,342,706 + -2.5 3,342,706 + +0.1 3,342,706 + +1.3 3,342,706 + -0.5 3,342,707 - +1.3 3,342,707 - +1.4 3,342,707 - -0.8 3,343,226 - -0.2 3,343,269 + +0.2 3,343,269 + +0.2 3,343,270 - -0.3 3,343,302 + -1.4 3,343,303 - -0.2 3,343,338 - -0.3 3,343,339 + +0.7 3,343,340 - -0.3 3,343,486 + +0.3 3,343,486 + -0.4 3,343,504 - -0.9 3,343,525 + -0.2 3,343,525 + +1.2 3,343,555 - -0.5 3,343,586 + -2.5 3,343,659 - PP_5541 0.18 +1.1 3,343,741 + PP_5541 0.43 -2.0 3,343,741 + PP_5541 0.43 +1.4 3,343,746 - PP_5541 0.45 -1.0 3,343,779 + PP_5541 0.55 +0.8 3,343,780 - PP_5541 0.55 +1.4 3,343,780 - PP_5541 0.55 -0.6 3,343,780 - PP_5541 0.55 +0.4 3,343,970 + +1.5 3,343,970 + +0.8 3,343,971 - -0.0 3,344,045 - PP_5542 0.16 -1.5 3,344,045 - PP_5542 0.16 +0.3 3,344,056 + PP_5542 0.20 +0.3 3,344,057 - PP_5542 0.20 -2.8 3,344,066 - PP_5542 0.24 -0.1 3,344,133 - PP_5542 0.49 -1.1 3,344,231 - PP_5542 0.85 -2.1 3,344,231 - PP_5542 0.85 +1.5 3,344,410 + -0.1 3,344,411 - -1.1 3,344,545 - PP_2941 0.26 -1.6 3,344,545 - PP_2941 0.26 +1.6 3,344,656 + PP_2941 0.40 +0.9 3,344,656 + PP_2941 0.40 +2.0 3,344,657 - PP_2941 0.40 -0.0 3,344,657 - PP_2941 0.40 -0.3 3,344,660 - PP_2941 0.40 +0.3 3,344,660 - PP_2941 0.40 -1.2 3,344,698 + PP_2941 0.45 +0.7 3,344,699 - PP_2941 0.45 +0.6 3,344,903 - PP_2941 0.71 -0.4
Or see this region's nucleotide sequence