Strain Fitness in Pseudomonas putida KT2440 around PP_5530

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,850,501	-		PP_2503	0.40	-0.7
2,850,555	-		PP_2503	0.47	-0.9
2,850,555	-		PP_2503	0.47	+0.6
2,850,867	-				+0.2
2,850,867	-				+0.4
2,850,867	-				-0.5
2,850,919	+				+0.1
2,850,920	-				-1.1
2,850,920	-				+0.5
2,850,938	+				-0.7
2,850,938	+				+0.6
2,850,941	+				-0.3
2,850,941	+				-0.1
2,850,941	+				-0.3
2,850,942	-				+0.2
2,850,942	-				-0.4
2,850,942	-				+0.9
2,851,003	+				-1.5
2,851,003	+				-1.5
2,851,003	+				-0.5
2,851,003	+				-1.6
2,851,003	+				-0.5
2,851,004	-				-1.4
2,851,004	-				-1.0
2,851,004	-				-0.0
2,851,006	+				+0.1
2,851,006	+				+0.3
2,851,006	+				+1.0
2,851,007	-				+0.0
2,851,007	-				-0.1
2,851,007	-				-2.2
2,851,007	-				+1.1
2,851,133	-		PP_2504	0.59	-1.1
2,851,159	+		PP_2504	0.71	-1.1
2,851,193	+		PP_2504	0.87	+0.9
2,851,318	+		PP_5530	0.48	-1.8
2,851,318	+		PP_5530	0.48	-0.8
2,851,318	+		PP_5530	0.48	-0.0
2,851,318	+		PP_5530	0.48	+0.3
2,851,318	+		PP_5530	0.48	+1.1
2,851,318	+		PP_5530	0.48	+0.1
2,851,319	-		PP_5530	0.48	+0.4
2,851,319	-		PP_5530	0.48	+0.7
2,851,319	-		PP_5530	0.48	+1.3
2,851,319	-		PP_5530	0.48	+1.1
2,851,319	-		PP_5530	0.48	+0.8
2,851,344	+		PP_5530	0.60	-0.0
2,851,426	+				-0.5
2,851,460	+				-0.9
2,851,460	+				-1.2
2,851,461	-				-0.3
2,851,518	+				+1.7
2,851,555	+				-0.2
2,851,555	+				-0.7
2,851,556	-				-0.9
2,851,559	+				+1.7
2,851,570	+				-0.2
2,851,570	+				+0.6
2,851,570	+				-2.1
2,851,571	-				+1.4
2,851,571	-				+0.8
2,851,571	-				-1.7
2,851,571	-				-0.8
2,851,615	-				+1.5
2,851,670	+		PP_5531	0.19	-0.7
2,851,671	-		PP_5531	0.19	-0.4
2,851,739	+		PP_5531	0.32	-1.2
2,851,739	+		PP_5531	0.32	-2.4
2,851,739	+		PP_5531	0.32	-1.7
2,851,778	-		PP_5531	0.40	+0.5
2,851,851	+		PP_5531	0.54	+0.7
2,851,852	-		PP_5531	0.54	+0.3
2,851,852	-		PP_5531	0.54	-1.2
2,851,864	+		PP_5531	0.56	-0.7
2,851,884	+		PP_5531	0.60	+0.1
2,851,884	+		PP_5531	0.60	+1.3
2,851,885	-		PP_5531	0.61	+0.7
2,851,885	-		PP_5531	0.61	-1.1
2,851,920	+		PP_5531	0.67	-0.8
2,851,921	-		PP_5531	0.67	+0.2
2,851,942	+		PP_5531	0.71	-1.6
2,851,959	-		PP_5531	0.75	-0.5
2,852,009	+		PP_5531	0.84	-0.1
2,852,009	+		PP_5531	0.84	-0.8
2,852,009	+		PP_5531	0.84	-0.8
2,852,010	-		PP_5531	0.85	+0.9
2,852,011	+		PP_5531	0.85	+0.5
2,852,055	-				+0.1
2,852,075	+				-0.8
2,852,075	+				+0.2
2,852,197	-				+0.5
2,852,197	-				-2.0
2,852,242	-				+2.9
2,852,264	-				-1.1
2,852,264	-				+0.1
2,852,286	+				-2.2
2,852,287	-				-0.1
2,852,287	-				-1.5
2,852,313	+				-0.4
2,852,314	-				-0.6
2,852,314	-				-0.6
2,852,314	-				+1.2
2,852,349	+				-0.8
2,852,349	+				+0.4
2,852,350	-				-0.0
2,852,372	+				-1.7
2,852,372	+				-0.5