Strain Fitness in Pseudomonas putida KT2440 around PP_5504

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,545,943	+				+1.7
2,546,103	-		PP_2238	0.63	-0.6
2,546,215	-		PP_2238	0.69	-2.7
2,546,215	-		PP_2238	0.69	+0.2
2,546,296	+		PP_2238	0.73	+1.5
2,546,296	+		PP_2238	0.73	+0.4
2,546,297	-				+1.4
2,546,297	-		PP_2238	0.73	+0.4
2,546,404	-		PP_2238	0.79	+1.2
2,546,404	-		PP_2238	0.79	-0.2
2,546,404	-		PP_2238	0.79	-2.5
2,546,433	+		PP_2238	0.81	-0.5
2,546,433	+		PP_2238	0.81	+1.1
2,546,434	-		PP_2238	0.81	+1.5
2,546,537	+		PP_2238	0.86	-0.6
2,546,537	+		PP_2238	0.86	+0.7
2,546,538	-		PP_2238	0.86	+1.1
2,546,538	-		PP_2238	0.86	-1.5
2,546,538	-		PP_2238	0.86	-0.6
2,546,728	+				-0.8
2,546,728	+				-1.4
2,546,728	+				+1.0
2,546,729	-				-0.1
2,546,729	-				+0.2
2,546,729	-				+0.1
2,546,729	-				+0.0
2,546,729	-				-0.6
2,546,729	-				-0.1
2,546,729	-				+0.1
2,546,768	-				-1.6
2,546,786	+				+0.6
2,546,786	+				-1.1
2,546,786	+				-2.7
2,546,787	-				+0.3
2,546,787	-				+1.5
2,546,842	+				-0.3
2,546,842	+				+0.3
2,546,846	+				+0.8
2,546,846	+				+0.6
2,546,847	-				+0.8
2,546,876	-		PP_5504	0.12	+0.1
2,546,876	-		PP_5504	0.12	-2.6
2,546,902	+		PP_5504	0.22	-1.6
2,546,903	-		PP_5504	0.22	+1.2
2,546,903	-		PP_5504	0.22	+0.2
2,546,903	-		PP_5504	0.22	-0.5
2,546,987	-		PP_5504	0.52	+0.7
2,547,027	-		PP_5504	0.66	-1.6
2,547,027	-		PP_5504	0.66	-0.7
2,547,132	+				-2.1
2,547,133	-				-0.8
2,547,219	+				-0.8
2,547,219	+				+0.0
2,547,220	-				+1.9
2,547,220	-				-0.3
2,547,220	-				+1.6
2,547,223	+				-0.0
2,547,223	+				-0.5
2,547,224	-				-0.1
2,547,228	+				-0.7
2,547,229	-				-1.7
2,547,229	-				-1.6
2,547,229	-				+0.8
2,547,253	+				+0.1
2,547,253	+				-1.0
2,547,253	+				-1.4
2,547,254	-				-0.5
2,547,273	+				-1.6
2,547,273	+				-2.0
2,547,274	-				-1.3
2,547,274	-				-1.4
2,547,274	-				-1.3
2,547,274	-				-0.4
2,547,379	+				-1.5
2,547,531	+		PP_2239	0.24	+1.4
2,547,531	+		PP_2239	0.24	+0.3
2,547,551	+		PP_2239	0.27	-0.9
2,547,552	-		PP_2239	0.27	-0.9
2,547,552	-		PP_2239	0.27	-1.3
2,547,552	-		PP_2239	0.27	+0.9
2,547,552	-		PP_2239	0.27	-0.4
2,547,711	+		PP_2239	0.45	+0.6
2,547,711	+		PP_2239	0.45	-0.0
2,547,711	+		PP_2239	0.45	-0.1
2,547,711	+		PP_2239	0.45	-0.2
2,547,712	-		PP_2239	0.45	+0.7
2,547,744	+		PP_2239	0.48	-0.1
2,547,744	+		PP_2239	0.48	-0.8
2,547,744	+		PP_2239	0.48	+1.1
2,547,904	+		PP_2239	0.66	+0.6
2,547,915	-		PP_2239	0.68	+0.6
2,547,931	+		PP_2239	0.69	+0.1
2,547,932	-		PP_2239	0.70	-0.3
2,547,932	-		PP_2239	0.70	+0.6
2,547,932	-		PP_2239	0.70	+0.7
2,547,933	+		PP_2239	0.70	+0.7
2,547,933	+		PP_2239	0.70	+0.2
2,547,933	+		PP_2239	0.70	+0.2
2,547,933	+		PP_2239	0.70	+1.3
2,547,933	+		PP_2239	0.70	+0.5
2,547,933	+		PP_2239	0.70	-1.2
2,547,933	+		PP_2239	0.70	-3.0
2,547,934	-		PP_2239	0.70	-0.5
2,547,934	-		PP_2239	0.70	+0.9
2,547,934	-		PP_2239	0.70	+0.6
2,547,934	-		PP_2239	0.70	-1.5
2,547,934	-		PP_2239	0.70	+1.2
2,547,934	-		PP_2239	0.70	-1.4
2,547,969	+		PP_2239	0.74	+0.8
2,547,970	-		PP_2239	0.74	-0.0
2,547,970	-		PP_2239	0.74	-0.9
2,547,970	-		PP_2239	0.74	+0.7
2,547,970	-		PP_2239	0.74	-2.1
2,548,025	+		PP_2239	0.80	+1.5
2,548,025	+		PP_2239	0.80	-0.5
2,548,025	+		PP_2239	0.80	-1.2
2,548,026	-		PP_2239	0.80	-0.2
2,548,026	-		PP_2239	0.80	+0.8
2,548,027	+		PP_2239	0.80	+0.9
2,548,027	+		PP_2239	0.80	+0.3
2,548,027	+		PP_2239	0.80	-1.1
2,548,028	-		PP_2239	0.80	+0.4
2,548,028	-		PP_2239	0.80	+0.8
2,548,069	+		PP_2239	0.85	-1.6
2,548,070	-		PP_2239	0.85	+1.7
2,548,070	-		PP_2239	0.85	-0.1
2,548,070	-		PP_2239	0.85	-0.8