Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1918 and PP_5482 are separated by 821 nucleotides PP_5482 and PP_1920 are separated by 163 nucleotides
PP_1918: PP_1918 - septation protein, at 2,161,053 to 2,162,318
_1918
PP_5482: PP_5482 - protein of unknown function, at 2,163,140 to 2,164,153
_5482
PP_1920: PP_1920 - conserved protein of unknown function, at 2,164,317 to 2,165,537
_1920
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2162.316 kb on + strand at 2162.316 kb on + strand at 2162.316 kb on + strand at 2162.316 kb on + strand at 2162.317 kb on - strand at 2162.317 kb on - strand at 2162.342 kb on + strand at 2162.343 kb on - strand at 2162.345 kb on + strand at 2162.345 kb on + strand at 2162.346 kb on - strand at 2162.346 kb on - strand at 2162.454 kb on - strand at 2162.630 kb on - strand at 2162.632 kb on - strand at 2162.632 kb on - strand at 2162.640 kb on - strand at 2162.723 kb on - strand at 2162.723 kb on - strand at 2162.737 kb on + strand at 2162.807 kb on - strand at 2162.873 kb on + strand at 2162.873 kb on + strand at 2162.873 kb on + strand at 2162.874 kb on - strand at 2162.902 kb on + strand at 2162.902 kb on + strand at 2162.902 kb on + strand at 2162.930 kb on + strand at 2162.930 kb on + strand at 2162.930 kb on + strand at 2162.931 kb on - strand at 2162.931 kb on - strand at 2162.937 kb on + strand at 2162.937 kb on + strand at 2162.937 kb on + strand at 2162.937 kb on + strand at 2162.937 kb on + strand at 2162.938 kb on - strand at 2162.938 kb on - strand at 2162.938 kb on - strand at 2162.939 kb on + strand at 2162.939 kb on + strand at 2162.939 kb on + strand at 2162.939 kb on + strand at 2162.939 kb on + strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.940 kb on - strand at 2162.965 kb on + strand at 2163.011 kb on + strand at 2163.011 kb on + strand at 2163.011 kb on + strand at 2163.113 kb on + strand at 2163.114 kb on - strand at 2163.114 kb on - strand at 2163.114 kb on - strand at 2163.161 kb on - strand at 2163.188 kb on - strand at 2163.202 kb on - strand at 2163.205 kb on + strand at 2163.206 kb on - strand at 2163.225 kb on + strand at 2163.225 kb on + strand at 2163.225 kb on + strand at 2163.349 kb on + strand, within PP_5482 at 2163.383 kb on - strand, within PP_5482 at 2163.422 kb on + strand, within PP_5482 at 2163.422 kb on + strand, within PP_5482 at 2163.452 kb on + strand, within PP_5482 at 2163.610 kb on + strand, within PP_5482 at 2163.644 kb on - strand, within PP_5482 at 2163.646 kb on - strand, within PP_5482 at 2163.786 kb on + strand, within PP_5482 at 2163.787 kb on - strand, within PP_5482 at 2163.897 kb on + strand, within PP_5482 at 2163.980 kb on + strand, within PP_5482 at 2163.984 kb on - strand, within PP_5482 at 2163.984 kb on - strand, within PP_5482 at 2164.147 kb on + strand at 2164.148 kb on - strand at 2164.223 kb on + strand at 2164.364 kb on + strand at 2164.424 kb on + strand at 2164.482 kb on + strand, within PP_1920 at 2164.599 kb on - strand, within PP_1920 at 2164.632 kb on + strand, within PP_1920 at 2164.723 kb on - strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.852 kb on + strand, within PP_1920 at 2164.931 kb on + strand, within PP_1920 at 2165.143 kb on - strand, within PP_1920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,162,316 + -0.4 2,162,316 + -1.1 2,162,316 + -1.6 2,162,316 + +0.1 2,162,317 - +0.5 2,162,317 - -0.1 2,162,342 + -1.3 2,162,343 - +0.2 2,162,345 + +0.3 2,162,345 + -2.1 2,162,346 - +1.3 2,162,346 - -0.2 2,162,454 - -0.2 2,162,630 - +0.0 2,162,632 - -0.3 2,162,632 - -0.9 2,162,640 - +0.7 2,162,723 - -0.2 2,162,723 - -0.6 2,162,737 + +1.0 2,162,807 - -1.0 2,162,873 + -0.5 2,162,873 + -1.8 2,162,873 + +0.6 2,162,874 - +0.9 2,162,902 + -0.0 2,162,902 + -0.4 2,162,902 + -0.2 2,162,930 + +0.2 2,162,930 + +1.2 2,162,930 + +0.4 2,162,931 - +0.6 2,162,931 - -1.2 2,162,937 + +1.2 2,162,937 + +0.4 2,162,937 + +0.8 2,162,937 + -1.9 2,162,937 + +0.7 2,162,938 - -1.3 2,162,938 - -0.6 2,162,938 - -1.1 2,162,939 + +0.1 2,162,939 + -1.0 2,162,939 + -0.8 2,162,939 + -1.4 2,162,939 + -0.9 2,162,940 - +1.4 2,162,940 - -0.4 2,162,940 - -0.2 2,162,940 - +1.0 2,162,940 - +1.1 2,162,940 - -0.6 2,162,940 - -0.4 2,162,940 - +0.3 2,162,965 + +0.4 2,163,011 + +0.7 2,163,011 + +0.6 2,163,011 + -1.3 2,163,113 + -0.9 2,163,114 - -1.4 2,163,114 - +1.8 2,163,114 - +2.0 2,163,161 - +0.2 2,163,188 - +0.3 2,163,202 - -2.7 2,163,205 + +0.9 2,163,206 - +0.0 2,163,225 + +0.9 2,163,225 + +1.0 2,163,225 + -0.9 2,163,349 + PP_5482 0.21 -0.2 2,163,383 - PP_5482 0.24 +1.5 2,163,422 + PP_5482 0.28 +1.9 2,163,422 + PP_5482 0.28 +1.2 2,163,452 + PP_5482 0.31 -0.2 2,163,610 + PP_5482 0.46 -0.7 2,163,644 - PP_5482 0.50 +0.9 2,163,646 - PP_5482 0.50 -0.7 2,163,786 + PP_5482 0.64 +1.3 2,163,787 - PP_5482 0.64 -0.7 2,163,897 + PP_5482 0.75 -0.5 2,163,980 + PP_5482 0.83 +0.9 2,163,984 - PP_5482 0.83 -1.1 2,163,984 - PP_5482 0.83 +2.0 2,164,147 + -1.8 2,164,148 - -0.7 2,164,223 + -0.5 2,164,364 + +0.9 2,164,424 + -0.9 2,164,482 + PP_1920 0.14 +0.5 2,164,599 - PP_1920 0.23 -0.7 2,164,632 + PP_1920 0.26 -2.0 2,164,723 - PP_1920 0.33 -0.2 2,164,738 + PP_1920 0.34 +0.2 2,164,738 + PP_1920 0.34 -2.0 2,164,739 - PP_1920 0.35 +0.5 2,164,739 - PP_1920 0.35 -1.9 2,164,739 - PP_1920 0.35 +0.4 2,164,852 + PP_1920 0.44 +0.8 2,164,931 + PP_1920 0.50 -0.8 2,165,143 - PP_1920 0.68 -0.1
Or see this region's nucleotide sequence