Strain Fitness in Pseudomonas putida KT2440 around PP_5480

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,123,109	+		PP_1886	0.66	+0.7
2,123,109	+		PP_1886	0.66	+0.1
2,123,110	-		PP_1886	0.66	+0.4
2,123,110	-		PP_1886	0.66	-0.9
2,123,110	-		PP_1886	0.66	+0.5
2,123,110	-		PP_1886	0.66	+1.2
2,123,110	-		PP_1886	0.66	+0.1
2,123,245	-		PP_1886	0.71	+1.1
2,123,345	-		PP_1886	0.74	+0.1
2,123,345	-		PP_1886	0.74	-0.1
2,123,345	-		PP_1886	0.74	-0.3
2,123,396	+		PP_1886	0.76	-1.8
2,123,396	+		PP_1886	0.76	-1.1
2,123,397	-		PP_1886	0.76	-1.5
2,123,443	-		PP_1886	0.78	+0.8
2,123,443	-		PP_1886	0.78	-0.0
2,123,459	+		PP_1886	0.78	+2.0
2,123,474	+		PP_1886	0.79	-0.5
2,123,475	-		PP_1886	0.79	-0.3
2,123,475	-		PP_1886	0.79	-0.4
2,123,475	-		PP_1886	0.79	-1.8
2,123,513	-		PP_1886	0.80	+0.9
2,123,588	+		PP_1886	0.82	-0.5
2,123,606	+		PP_1886	0.83	-1.0
2,123,607	-		PP_1886	0.83	+1.1
2,123,699	+		PP_1886	0.86	+0.1
2,123,699	+		PP_1886	0.86	-0.1
2,123,700	-		PP_1886	0.86	-0.2
2,123,822	+				+0.5
2,123,881	+				+0.9
2,123,894	-				+1.2
2,123,978	+				+0.9
2,123,978	+				-0.4
2,123,979	-				-1.1
2,124,010	+				+1.2
2,124,096	+				-1.1
2,124,096	+				-0.1
2,124,097	-				-0.4
2,124,097	-				-0.4
2,124,189	+		PP_5480	0.18	+2.3
2,124,211	-		PP_5480	0.22	-2.4
2,124,224	+		PP_5480	0.25	-0.1
2,124,244	-		PP_5480	0.29	-0.3
2,124,265	-		PP_5480	0.33	+1.1
2,124,334	-		PP_5480	0.46	-1.5
2,124,339	-		PP_5480	0.47	-2.0
2,124,391	+		PP_5480	0.58	-1.6
2,124,523	-		PP_5480	0.84	+0.5
2,124,535	-		PP_5480	0.86	-1.5
2,124,600	+				+0.5
2,124,681	+				-0.3
2,124,682	-				+0.5
2,124,685	+				-0.7
2,124,685	+				+1.5
2,124,686	-				-0.3
2,124,856	+				+0.7
2,124,856	+				+0.2
2,124,857	-				-1.6
2,124,908	+				+0.4
2,124,969	+				+0.4
2,124,970	-				-0.2
2,124,970	-				-1.0
2,124,970	-				-1.8
2,124,970	-				-2.4
2,125,125	+		PP_1887	0.13	+0.8
2,125,126	-		PP_1887	0.13	-0.8
2,125,211	+		PP_1887	0.15	-0.4
2,125,356	+		PP_1887	0.19	-2.1
2,125,356	+		PP_1887	0.19	-1.4
2,125,356	+		PP_1887	0.19	-0.2
2,125,356	+		PP_1887	0.19	-0.1
2,125,357	-		PP_1887	0.19	+1.3
2,125,357	-		PP_1887	0.19	-1.7
2,125,374	+		PP_1887	0.19	-0.9
2,125,374	+		PP_1887	0.19	-1.1
2,125,384	+		PP_1887	0.19	-0.1
2,125,385	-		PP_1887	0.19	+0.5
2,125,385	-		PP_1887	0.19	-1.4
2,125,404	+		PP_1887	0.20	-1.5
2,125,404	+		PP_1887	0.20	+0.4
2,125,405	-		PP_1887	0.20	-1.3
2,125,405	-		PP_1887	0.20	+1.7
2,125,405	-		PP_1887	0.20	+0.3
2,125,428	+		PP_1887	0.21	+2.3
2,125,445	+		PP_1887	0.21	+0.9
2,125,445	+		PP_1887	0.21	-1.3
2,125,446	-		PP_1887	0.21	+0.7
2,125,446	-		PP_1887	0.21	-0.8
2,125,543	-		PP_1887	0.24	+0.3
2,125,548	+		PP_1887	0.24	+0.1