Strain Fitness in Pseudomonas putida KT2440 around PP_5423

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0047 and PP_0048 are separated by 117 nucleotidesPP_0048 and PP_5423 are separated by 101 nucleotidesPP_5423 and PP_0049 are separated by 189 nucleotides PP_0047: PP_0047 - response regulator, at 54,227 to 54,901 _0047 PP_0048: PP_0048 - conserved protein of unknown function, at 55,019 to 55,342 _0048 PP_5423: PP_5423 - conserved membrane protein of unknown function, at 55,444 to 55,971 _5423 PP_0049: PP_0049 - conserved protein of unknown function, at 56,161 to 57,627 _0049 Position (kb) 55 56Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 54.449 kb on - strand, within PP_0047at 54.600 kb on + strand, within PP_0047at 54.601 kb on - strand, within PP_0047at 54.689 kb on + strand, within PP_0047at 54.699 kb on + strand, within PP_0047at 54.718 kb on + strand, within PP_0047at 54.799 kb on - strand, within PP_0047at 54.831 kb on + strand, within PP_0047at 54.832 kb on - strand, within PP_0047at 54.832 kb on - strand, within PP_0047at 54.862 kb on + strandat 54.863 kb on - strandat 54.900 kb on - strandat 55.016 kb on + strandat 55.017 kb on - strandat 55.025 kb on + strandat 55.025 kb on + strandat 55.027 kb on + strandat 55.028 kb on - strandat 55.028 kb on - strandat 55.214 kb on + strand, within PP_0048at 55.377 kb on - strandat 55.465 kb on - strandat 55.537 kb on + strand, within PP_5423at 55.867 kb on - strand, within PP_5423at 55.877 kb on - strand, within PP_5423at 55.981 kb on + strandat 55.981 kb on + strandat 55.982 kb on - strandat 55.993 kb on + strandat 56.007 kb on - strandat 56.126 kb on - strandat 56.126 kb on - strandat 56.214 kb on + strandat 56.251 kb on + strandat 56.251 kb on + strandat 56.258 kb on - strandat 56.293 kb on + strandat 56.320 kb on + strand, within PP_0049at 56.331 kb on + strand, within PP_0049at 56.344 kb on + strand, within PP_0049at 56.344 kb on + strand, within PP_0049at 56.345 kb on - strand, within PP_0049at 56.345 kb on - strand, within PP_0049at 56.345 kb on - strand, within PP_0049at 56.345 kb on - strand, within PP_0049at 56.352 kb on + strand, within PP_0049at 56.365 kb on + strand, within PP_0049at 56.394 kb on - strand, within PP_0049at 56.399 kb on + strand, within PP_0049at 56.421 kb on - strand, within PP_0049at 56.434 kb on + strand, within PP_0049at 56.435 kb on - strand, within PP_0049at 56.504 kb on + strand, within PP_0049at 56.504 kb on + strand, within PP_0049at 56.504 kb on + strand, within PP_0049at 56.504 kb on + strand, within PP_0049at 56.505 kb on - strand, within PP_0049at 56.505 kb on - strand, within PP_0049at 56.518 kb on + strand, within PP_0049at 56.519 kb on - strand, within PP_0049at 56.612 kb on - strand, within PP_0049at 56.616 kb on + strand, within PP_0049at 56.616 kb on + strand, within PP_0049at 56.617 kb on - strand, within PP_0049at 56.617 kb on - strand, within PP_0049at 56.617 kb on - strand, within PP_0049at 56.620 kb on + strandat 56.620 kb on + strand, within PP_0049at 56.621 kb on - strand, within PP_0049at 56.641 kb on + strand, within PP_0049at 56.668 kb on + strand, within PP_0049at 56.668 kb on + strand, within PP_0049at 56.669 kb on - strand, within PP_0049at 56.669 kb on - strand, within PP_0049at 56.714 kb on + strand, within PP_0049at 56.714 kb on + strand, within PP_0049at 56.714 kb on + strand, within PP_0049at 56.714 kb on + strand, within PP_0049at 56.714 kb on + strand, within PP_0049at 56.720 kb on + strand, within PP_0049at 56.720 kb on + strand, within PP_0049at 56.720 kb on + strand, within PP_0049at 56.720 kb on + strand, within PP_0049at 56.721 kb on - strand, within PP_0049at 56.796 kb on + strand, within PP_0049at 56.796 kb on + strand, within PP_0049at 56.822 kb on + strand, within PP_0049

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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54,449 - PP_0047 0.33 +0.1
54,600 + PP_0047 0.55 +0.9
54,601 - PP_0047 0.55 -1.0
54,689 + PP_0047 0.68 +2.5
54,699 + PP_0047 0.70 -0.1
54,718 + PP_0047 0.73 -0.1
54,799 - PP_0047 0.85 +1.0
54,831 + PP_0047 0.89 +0.1
54,832 - PP_0047 0.90 +0.5
54,832 - PP_0047 0.90 +1.1
54,862 + -0.5
54,863 - -0.6
54,900 - -0.6
55,016 + -1.1
55,017 - -2.8
55,025 + -1.0
55,025 + +0.7
55,027 + -0.7
55,028 - -0.4
55,028 - -1.2
55,214 + PP_0048 0.60 -0.4
55,377 - -1.3
55,465 - +0.1
55,537 + PP_5423 0.18 -1.1
55,867 - PP_5423 0.80 +2.3
55,877 - PP_5423 0.82 -0.7
55,981 + -0.6
55,981 + -0.7
55,982 - +0.3
55,993 + +0.5
56,007 - -1.1
56,126 - -0.8
56,126 - -0.8
56,214 + -0.0
56,251 + +1.0
56,251 + -0.4
56,258 - +1.5
56,293 + -0.5
56,320 + PP_0049 0.11 +0.4
56,331 + PP_0049 0.12 -0.2
56,344 + PP_0049 0.12 -1.0
56,344 + PP_0049 0.12 +0.5
56,345 - PP_0049 0.13 -1.3
56,345 - PP_0049 0.13 -1.6
56,345 - PP_0049 0.13 -0.7
56,345 - PP_0049 0.13 -0.8
56,352 + PP_0049 0.13 +1.0
56,365 + PP_0049 0.14 -0.8
56,394 - PP_0049 0.16 +0.6
56,399 + PP_0049 0.16 +0.3
56,421 - PP_0049 0.18 +0.4
56,434 + PP_0049 0.19 +0.4
56,435 - PP_0049 0.19 +0.6
56,504 + PP_0049 0.23 -2.1
56,504 + PP_0049 0.23 -1.2
56,504 + PP_0049 0.23 -0.4
56,504 + PP_0049 0.23 -0.3
56,505 - PP_0049 0.23 -0.9
56,505 - PP_0049 0.23 +0.2
56,518 + PP_0049 0.24 -0.8
56,519 - PP_0049 0.24 -0.4
56,612 - PP_0049 0.31 +0.2
56,616 + PP_0049 0.31 -1.4
56,616 + PP_0049 0.31 -0.8
56,617 - PP_0049 0.31 -1.7
56,617 - PP_0049 0.31 -0.1
56,617 - PP_0049 0.31 -0.6
56,620 + +0.9
56,620 + PP_0049 0.31 -0.9
56,621 - PP_0049 0.31 -1.7
56,641 + PP_0049 0.33 +0.6
56,668 + PP_0049 0.35 +1.3
56,668 + PP_0049 0.35 -1.8
56,669 - PP_0049 0.35 -0.2
56,669 - PP_0049 0.35 -0.3
56,714 + PP_0049 0.38 +0.4
56,714 + PP_0049 0.38 +2.0
56,714 + PP_0049 0.38 +0.0
56,714 + PP_0049 0.38 -0.6
56,714 + PP_0049 0.38 -0.9
56,720 + PP_0049 0.38 -0.6
56,720 + PP_0049 0.38 -0.2
56,720 + PP_0049 0.38 +0.5
56,720 + PP_0049 0.38 -0.4
56,721 - PP_0049 0.38 +0.5
56,796 + PP_0049 0.43 +0.1
56,796 + PP_0049 0.43 +0.6
56,822 + PP_0049 0.45 -0.0

Or see this region's nucleotide sequence