Strain Fitness in Pseudomonas putida KT2440 around PP_5351

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
6,098,309	-		PP_5350	0.10	+0.6
6,098,388	+		PP_5350	0.19	-1.0
6,098,389	-		PP_5350	0.19	-0.4
6,098,389	-		PP_5350	0.19	+0.1
6,098,389	-		PP_5350	0.19	-1.3
6,098,435	+		PP_5350	0.25	-1.3
6,098,436	-		PP_5350	0.25	+2.3
6,098,545	+		PP_5350	0.37	-0.5
6,098,611	-		PP_5350	0.45	+0.7
6,098,611	-		PP_5350	0.45	-1.6
6,098,611	-		PP_5350	0.45	-1.0
6,098,689	-				+1.9
6,098,689	-		PP_5350	0.54	-0.7
6,098,808	+		PP_5350	0.68	+1.6
6,098,954	-		PP_5350	0.84	+0.1
6,099,071	-				+1.8
6,099,103	+				+1.6
6,099,125	-				-0.3
6,099,125	-				-1.8
6,099,136	-				+0.3
6,099,136	-				+0.8
6,099,139	+				-0.0
6,099,139	+				+0.8
6,099,140	-				+0.0
6,099,142	-				+0.2
6,099,144	+				+0.9
6,099,144	+				+0.8
6,099,197	+				+0.4
6,099,197	+				-1.0
6,099,197	+				+0.6
6,099,198	-				+1.0
6,099,198	-				-0.8
6,099,267	+				+1.0
6,099,267	+				-0.8
6,099,268	-				+2.0
6,099,348	+		PP_5351	0.12	+0.4
6,099,349	-		PP_5351	0.12	+0.2
6,099,349	-		PP_5351	0.12	+0.6
6,099,441	+		PP_5351	0.18	-0.6
6,099,442	-		PP_5351	0.18	+0.7
6,099,442	-		PP_5351	0.18	-0.8
6,099,493	-		PP_5351	0.22	-1.8
6,099,493	-		PP_5351	0.22	-0.6
6,099,528	+		PP_5351	0.24	+2.0
6,099,528	+		PP_5351	0.24	+0.1
6,099,529	-		PP_5351	0.24	+0.8
6,099,715	-		PP_5351	0.37	+0.8
6,099,839	-		PP_5351	0.45	-1.3
6,099,855	+		PP_5351	0.47	-0.2
6,099,855	+		PP_5351	0.47	-1.7
6,099,856	-		PP_5351	0.47	+0.1
6,099,856	-		PP_5351	0.47	-0.2
6,099,856	-		PP_5351	0.47	-0.1
6,099,859	-		PP_5351	0.47	-1.2
6,099,876	-		PP_5351	0.48	-1.4
6,099,962	-				+1.3
6,100,044	+		PP_5351	0.59	-1.8
6,100,083	+		PP_5351	0.62	-0.3
6,100,084	-		PP_5351	0.62	+0.2
6,100,086	+		PP_5351	0.62	-0.4
6,100,086	+		PP_5351	0.62	-1.5
6,100,086	+		PP_5351	0.62	-0.0
6,100,087	-		PP_5351	0.62	-2.0
6,100,087	-		PP_5351	0.62	-0.6
6,100,182	+		PP_5351	0.69	-2.0
6,100,182	+		PP_5351	0.69	+0.2
6,100,183	-		PP_5351	0.69	+0.3
6,100,291	+		PP_5351	0.76	-0.4
6,100,291	+		PP_5351	0.76	-0.1
6,100,291	+		PP_5351	0.76	-0.5
6,100,291	+		PP_5351	0.76	-1.5
6,100,382	+		PP_5351	0.83	+0.0
6,100,383	-		PP_5351	0.83	-1.0
6,100,425	+		PP_5351	0.86	+0.2
6,100,426	-		PP_5351	0.86	+1.2
6,100,510	+				+1.0
6,100,511	-				+1.0
6,100,668	+				-0.7
6,100,668	+				-0.9
6,100,797	-				+0.9
6,100,802	+				+0.2
6,100,803	-				+0.5
6,100,807	+				-0.2
6,100,811	+				-1.7
6,100,837	+				-1.2
6,101,331	+		PP_5352	0.22	-0.8
6,101,377	+		PP_5352	0.24	-1.8
6,101,377	+		PP_5352	0.24	-0.2
6,101,377	+		PP_5352	0.24	+0.3
6,101,377	+		PP_5352	0.24	-0.5
6,101,377	+		PP_5352	0.24	-0.8
6,101,377	+		PP_5352	0.24	-0.4
6,101,377	+		PP_5352	0.24	-1.4
6,101,378	-		PP_5352	0.24	+0.0
6,101,378	-		PP_5352	0.24	-1.5
6,101,378	-		PP_5352	0.24	+0.5
6,101,379	+		PP_5352	0.24	-1.5
6,101,485	+		PP_5352	0.29	-0.6
6,101,485	+		PP_5352	0.29	-0.1
6,101,486	-		PP_5352	0.29	-1.1
6,101,529	+		PP_5352	0.31	+0.3
6,101,617	+		PP_5352	0.35	-0.2
6,101,617	+		PP_5352	0.35	-1.2
6,101,618	-		PP_5352	0.35	-1.7
6,101,618	-		PP_5352	0.35	-1.4
6,101,618	-		PP_5352	0.35	-2.3