Strain Fitness in Pseudomonas putida KT2440 around PP_5331

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
6,078,642	+		PP_5330	0.55	-0.7
6,078,642	+		PP_5330	0.55	+0.2
6,078,642	+		PP_5330	0.55	-1.4
6,078,643	-		PP_5330	0.55	-1.3
6,078,666	+		PP_5330	0.57	+0.1
6,078,667	-		PP_5330	0.57	+1.6
6,078,879	+		PP_5330	0.73	-2.0
6,078,880	-		PP_5330	0.73	-1.6
6,078,880	-		PP_5330	0.73	-0.6
6,079,008	+		PP_5330	0.83	+0.2
6,079,123	-				+1.1
6,079,167	+				+0.2
6,079,167	+				-0.7
6,079,167	+				-0.2
6,079,244	+				-1.3
6,079,313	+				+1.5
6,079,344	+				+0.1
6,079,345	-				-1.8
6,079,375	+				-0.7
6,079,382	+				-1.6
6,079,382	+				-0.9
6,079,382	+				-0.7
6,079,382	+				-1.0
6,079,383	-				+0.6
6,079,383	-				-1.2
6,079,383	-				+3.1
6,079,384	+				-1.5
6,079,385	-				-0.3
6,079,385	-				+0.1
6,079,385	-				-1.0
6,079,385	-				-1.2
6,079,385	-				-0.0
6,079,385	-				-1.7
6,079,385	-				+0.8
6,079,385	-				+1.4
6,079,385	-				-1.1
6,079,385	-				-0.1
6,079,385	-				-0.7
6,079,386	+				-0.8
6,079,386	+				-2.1
6,079,386	+				-1.0
6,079,386	+				+2.0
6,079,386	+				+0.8
6,079,387	-				+2.7
6,079,387	-				-0.1
6,079,387	-				-0.4
6,079,400	-				+0.8
6,079,400	-				-0.4
6,079,405	+				+0.1
6,079,405	+				-0.0
6,079,405	+				-0.5
6,079,405	+				-1.8
6,079,405	+				+0.2
6,079,405	+				+2.6
6,079,405	+				-0.1
6,079,405	+				-0.1
6,079,406	-				+1.8
6,079,406	-				+0.5
6,079,406	-				-1.4
6,079,406	-				-0.3
6,079,416	+				-1.5
6,079,449	+				-1.5
6,079,450	-				+1.0
6,079,507	+				-3.0
6,079,507	+				+0.0
6,079,507	+				-0.3
6,079,507	+				-1.3
6,079,507	+				+1.0
6,079,508	-				-0.2
6,079,508	-				+0.6
6,079,508	-				+0.0
6,079,508	-				+1.5
6,079,508	-				-0.4
6,079,510	+				+0.7
6,079,510	+				+0.7
6,079,510	+				-0.4
6,079,511	-				+0.5
6,079,511	-				-0.1
6,079,511	-				+0.5
6,079,511	-				+2.4
6,079,824	+		PP_5331	0.59	-0.7
6,079,824	+		PP_5331	0.59	-1.0
6,079,824	+		PP_5331	0.59	+0.8
6,079,825	-		PP_5331	0.60	+0.3
6,079,826	+		PP_5331	0.60	-1.6
6,079,827	-		PP_5331	0.60	-1.2
6,079,927	+		PP_5331	0.85	-0.6
6,079,999	+				+1.7
6,080,168	+		PP_5332	0.12	-0.7
6,080,168	+		PP_5332	0.12	+0.3
6,080,169	-		PP_5332	0.12	-1.0
6,080,169	-		PP_5332	0.12	+0.2
6,080,178	+		PP_5332	0.13	-0.0
6,080,312	+		PP_5332	0.28	-0.5
6,080,313	-		PP_5332	0.29	-0.0
6,080,313	-		PP_5332	0.29	+1.5
6,080,313	-		PP_5332	0.29	-0.9
6,080,557	+		PP_5332	0.56	-0.0
6,080,557	+		PP_5332	0.56	+0.0
6,080,557	+		PP_5332	0.56	-0.4
6,080,557	+		PP_5332	0.56	+1.1
6,080,557	+		PP_5332	0.56	+1.8
6,080,557	+		PP_5332	0.56	+0.3
6,080,558	-		PP_5332	0.56	-0.6
6,080,558	-		PP_5332	0.56	-0.0
6,080,558	-		PP_5332	0.56	+0.0
6,080,558	-		PP_5332	0.56	+0.5
6,080,561	+		PP_5332	0.56	+0.0
6,080,561	+		PP_5332	0.56	+0.4
6,080,561	+		PP_5332	0.56	-0.8
6,080,562	-		PP_5332	0.56	-1.2
6,080,562	-		PP_5332	0.56	-1.4
6,080,618	+		PP_5332	0.63	+0.6
6,080,618	+		PP_5332	0.63	-1.4
6,080,618	+		PP_5332	0.63	+1.2
6,080,618	+		PP_5332	0.63	-0.7
6,080,619	-		PP_5332	0.63	-0.9
6,080,619	-		PP_5332	0.63	-0.9
6,080,619	-		PP_5332	0.63	-1.4
6,080,619	-		PP_5332	0.63	+0.2
6,080,621	-		PP_5332	0.63	-0.2
6,080,696	+		PP_5332	0.71	+0.4
6,080,753	-		PP_5332	0.78	-0.3
6,080,753	-		PP_5332	0.78	+0.7
6,080,948	-				-0.6