Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5168 and PP_5169 are separated by 3 nucleotides PP_5169 and PP_5170 are separated by 11 nucleotides
PP_5168: PP_5168 - sulfate/thiosulfate import ATP-binding protein, at 5,893,480 to 5,894,469
_5168
PP_5169: PP_5169 - sulfate ABC transporter, inner membrane subunit CysW, at 5,894,473 to 5,895,345
_5169
PP_5170: PP_5170 - sulfate ABC transporter, inner membrane subunit CysT, at 5,895,357 to 5,896,175
_5170
Position (kb)
5894
5895
5896 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.481 kb on + strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.482 kb on - strand at 5893.485 kb on + strand at 5893.486 kb on - strand at 5893.770 kb on - strand, within PP_5168 at 5893.796 kb on + strand, within PP_5168 at 5893.797 kb on - strand, within PP_5168 at 5894.222 kb on + strand, within PP_5168 at 5894.409 kb on + strand at 5894.410 kb on - strand at 5894.452 kb on - strand at 5894.505 kb on + strand at 5894.508 kb on + strand at 5894.509 kb on - strand at 5894.509 kb on - strand at 5894.597 kb on + strand, within PP_5169 at 5894.597 kb on + strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.598 kb on - strand, within PP_5169 at 5894.607 kb on - strand at 5894.703 kb on + strand, within PP_5169 at 5894.704 kb on - strand, within PP_5169 at 5894.720 kb on + strand, within PP_5169 at 5894.860 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.908 kb on + strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.909 kb on - strand, within PP_5169 at 5894.917 kb on - strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5894.972 kb on + strand, within PP_5169 at 5895.053 kb on + strand, within PP_5169 at 5895.054 kb on - strand, within PP_5169 at 5895.054 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.089 kb on - strand, within PP_5169 at 5895.111 kb on + strand, within PP_5169 at 5895.112 kb on - strand at 5895.209 kb on - strand, within PP_5169 at 5895.273 kb on + strand at 5895.274 kb on - strand at 5895.455 kb on - strand, within PP_5170 at 5895.455 kb on - strand, within PP_5170 at 5895.455 kb on - strand, within PP_5170 at 5895.460 kb on + strand, within PP_5170 at 5895.460 kb on + strand, within PP_5170 at 5895.461 kb on - strand, within PP_5170 at 5895.917 kb on - strand, within PP_5170 at 5895.917 kb on - strand, within PP_5170 at 5895.945 kb on - strand, within PP_5170 at 5896.111 kb on + strand at 5896.112 kb on - strand at 5896.112 kb on - strand at 5896.112 kb on - strand at 5896.112 kb on - strand at 5896.123 kb on + strand at 5896.124 kb on - strand at 5896.163 kb on + strand at 5896.163 kb on + strand at 5896.164 kb on - strand at 5896.164 kb on - strand at 5896.164 kb on - strand at 5896.175 kb on + strand at 5896.175 kb on + strand at 5896.175 kb on + strand at 5896.175 kb on + strand at 5896.175 kb on + strand at 5896.175 kb on + strand at 5896.176 kb on - strand at 5896.176 kb on - strand at 5896.176 kb on - strand at 5896.176 kb on - strand at 5896.179 kb on + strand at 5896.180 kb on - strand at 5896.198 kb on + strand at 5896.198 kb on + strand at 5896.199 kb on - strand at 5896.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,893,481 + -0.4 5,893,481 + -2.0 5,893,481 + -0.8 5,893,481 + -0.3 5,893,481 + +1.2 5,893,481 + +1.3 5,893,482 - -0.5 5,893,482 - +0.2 5,893,482 - +0.2 5,893,482 - +0.0 5,893,482 - +2.2 5,893,482 - +0.4 5,893,482 - -0.1 5,893,485 + -0.2 5,893,486 - +1.2 5,893,770 - PP_5168 0.29 +0.3 5,893,796 + PP_5168 0.32 -1.2 5,893,797 - PP_5168 0.32 +1.0 5,894,222 + PP_5168 0.75 +1.0 5,894,409 + +0.0 5,894,410 - +0.0 5,894,452 - -1.9 5,894,505 + +0.7 5,894,508 + +0.8 5,894,509 - +0.9 5,894,509 - +0.9 5,894,597 + PP_5169 0.14 -1.0 5,894,597 + PP_5169 0.14 +0.9 5,894,598 - PP_5169 0.14 +0.0 5,894,598 - PP_5169 0.14 -0.5 5,894,598 - PP_5169 0.14 +0.2 5,894,598 - PP_5169 0.14 +0.5 5,894,607 - +1.7 5,894,703 + PP_5169 0.26 +0.5 5,894,704 - PP_5169 0.26 -1.5 5,894,720 + PP_5169 0.28 +0.5 5,894,860 + PP_5169 0.44 +1.1 5,894,908 + PP_5169 0.50 +0.9 5,894,908 + PP_5169 0.50 -0.0 5,894,908 + PP_5169 0.50 +0.9 5,894,909 - PP_5169 0.50 +2.4 5,894,909 - PP_5169 0.50 +1.9 5,894,909 - PP_5169 0.50 +0.6 5,894,909 - PP_5169 0.50 -1.4 5,894,909 - PP_5169 0.50 +0.2 5,894,917 - PP_5169 0.51 +0.3 5,894,972 + PP_5169 0.57 +1.0 5,894,972 + PP_5169 0.57 +0.2 5,894,972 + PP_5169 0.57 -0.3 5,895,053 + PP_5169 0.66 -0.1 5,895,054 - PP_5169 0.67 -0.5 5,895,054 - PP_5169 0.67 +1.1 5,895,089 - PP_5169 0.71 +1.3 5,895,089 - PP_5169 0.71 -1.3 5,895,089 - PP_5169 0.71 -0.6 5,895,089 - PP_5169 0.71 +0.3 5,895,111 + PP_5169 0.73 +1.2 5,895,112 - +0.7 5,895,209 - PP_5169 0.84 +0.2 5,895,273 + +1.5 5,895,274 - +0.7 5,895,455 - PP_5170 0.12 +1.2 5,895,455 - PP_5170 0.12 -0.9 5,895,455 - PP_5170 0.12 -2.5 5,895,460 + PP_5170 0.13 -0.2 5,895,460 + PP_5170 0.13 -2.0 5,895,461 - PP_5170 0.13 -0.5 5,895,917 - PP_5170 0.68 -0.6 5,895,917 - PP_5170 0.68 -0.6 5,895,945 - PP_5170 0.72 -1.1 5,896,111 + -0.0 5,896,112 - +0.7 5,896,112 - +0.5 5,896,112 - -0.3 5,896,112 - +1.2 5,896,123 + -1.6 5,896,124 - -1.3 5,896,163 + -0.5 5,896,163 + -0.4 5,896,164 - -2.8 5,896,164 - -0.6 5,896,164 - -0.3 5,896,175 + -0.3 5,896,175 + -0.8 5,896,175 + -0.0 5,896,175 + +2.2 5,896,175 + -1.7 5,896,175 + -1.0 5,896,176 - -1.9 5,896,176 - +0.2 5,896,176 - -0.5 5,896,176 - -2.2 5,896,179 + +0.0 5,896,180 - -0.2 5,896,198 + +0.0 5,896,198 + -1.0 5,896,199 - +0.3 5,896,199 - +0.8
Or see this region's nucleotide sequence