Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5050 and PP_5051 are separated by 517 nucleotides PP_5051 and PP_5052 overlap by 14 nucleotides PP_5052 and PP_5053 are separated by 155 nucleotides PP_5053 and PP_5054 are separated by 39 nucleotides PP_5054 and PP_5055 are separated by 1 nucleotides
PP_5050: PP_5050 - transposase, at 5,757,011 to 5,757,976
_5050
PP_5051: PP_5051 - conserved protein of unknown function, at 5,758,494 to 5,758,931
_5051
PP_5052: PP_5052 - tRNA(Leu) mC34,mU34 2'-O-ribose methyltransferase, AdoMet-dependent, at 5,758,918 to 5,759,391
_5052
PP_5053: PP_5053 - Protein-export protein SecB, at 5,759,547 to 5,760,032
_5053
PP_5054: PP_5054 - glutaredoxin 3, at 5,760,072 to 5,760,326
_5054
PP_5055: PP_5055 - Sulfurtransferase, at 5,760,328 to 5,760,741
_5055
Position (kb) 5758
5759
5760 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5758.093 kb on + strand at 5758.116 kb on + strand at 5758.318 kb on - strand at 5758.324 kb on + strand at 5758.325 kb on - strand at 5758.325 kb on - strand at 5758.325 kb on - strand at 5758.351 kb on - strand at 5758.379 kb on - strand at 5758.411 kb on + strand at 5758.411 kb on + strand at 5758.411 kb on + strand at 5758.438 kb on - strand at 5758.438 kb on - strand at 5758.438 kb on - strand at 5758.439 kb on + strand at 5758.440 kb on - strand at 5758.467 kb on + strand at 5758.468 kb on - strand at 5758.496 kb on - strand at 5758.612 kb on - strand, within PP_5051 at 5758.796 kb on + strand, within PP_5051 at 5758.821 kb on + strand, within PP_5051 at 5758.992 kb on - strand, within PP_5052 at 5759.089 kb on + strand, within PP_5052 at 5759.164 kb on - strand, within PP_5052 at 5759.335 kb on - strand, within PP_5052 at 5759.353 kb on + strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.354 kb on - strand at 5759.433 kb on + strand at 5759.477 kb on + strand at 5759.545 kb on - strand at 5759.549 kb on - strand at 5759.549 kb on - strand at 5759.558 kb on + strand at 5759.559 kb on - strand at 5759.559 kb on - strand at 5759.582 kb on + strand at 5759.602 kb on + strand, within PP_5053 at 5759.603 kb on - strand, within PP_5053 at 5759.766 kb on - strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.962 kb on + strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5759.963 kb on - strand, within PP_5053 at 5760.043 kb on + strand at 5760.048 kb on + strand at 5760.048 kb on + strand at 5760.048 kb on + strand at 5760.118 kb on + strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.119 kb on - strand, within PP_5054 at 5760.269 kb on - strand, within PP_5054 at 5760.296 kb on - strand, within PP_5054 at 5760.307 kb on + strand at 5760.326 kb on + strand at 5760.326 kb on + strand at 5760.326 kb on + strand at 5760.327 kb on - strand at 5760.327 kb on - strand at 5760.327 kb on - strand at 5760.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,758,093 + -0.4 5,758,116 + -0.5 5,758,318 - +0.6 5,758,324 + +1.2 5,758,325 - -1.8 5,758,325 - -0.7 5,758,325 - -1.5 5,758,351 - -1.5 5,758,379 - +0.7 5,758,411 + -1.8 5,758,411 + -2.3 5,758,411 + +0.3 5,758,438 - -0.4 5,758,438 - +1.2 5,758,438 - -0.6 5,758,439 + +1.5 5,758,440 - -0.7 5,758,467 + +1.4 5,758,468 - -1.9 5,758,496 - -0.1 5,758,612 - PP_5051 0.27 -0.4 5,758,796 + PP_5051 0.69 -0.4 5,758,821 + PP_5051 0.75 +0.1 5,758,992 - PP_5052 0.16 -1.4 5,759,089 + PP_5052 0.36 +0.3 5,759,164 - PP_5052 0.52 -1.5 5,759,335 - PP_5052 0.88 -2.6 5,759,353 + +0.6 5,759,354 - +1.6 5,759,354 - +0.4 5,759,354 - +0.1 5,759,354 - -0.9 5,759,354 - -0.9 5,759,433 + +0.5 5,759,477 + +2.0 5,759,545 - +0.1 5,759,549 - -1.9 5,759,549 - -0.5 5,759,558 + +1.5 5,759,559 - +0.8 5,759,559 - +0.9 5,759,582 + -0.5 5,759,602 + PP_5053 0.11 +1.6 5,759,603 - PP_5053 0.12 +1.4 5,759,766 - PP_5053 0.45 -0.3 5,759,962 + PP_5053 0.85 -0.9 5,759,962 + PP_5053 0.85 -2.2 5,759,962 + PP_5053 0.85 -0.7 5,759,962 + +2.6 5,759,962 + PP_5053 0.85 -2.3 5,759,962 + PP_5053 0.85 -2.5 5,759,962 + PP_5053 0.85 -0.3 5,759,962 + PP_5053 0.85 -0.7 5,759,962 + PP_5053 0.85 -3.0 5,759,962 + PP_5053 0.85 -0.0 5,759,963 - PP_5053 0.86 +1.4 5,759,963 - PP_5053 0.86 +0.3 5,759,963 - PP_5053 0.86 +0.3 5,759,963 - PP_5053 0.86 -0.3 5,759,963 - PP_5053 0.86 +0.5 5,759,963 - PP_5053 0.86 -1.5 5,759,963 - PP_5053 0.86 -0.3 5,760,043 + +0.4 5,760,048 + +0.6 5,760,048 + -0.5 5,760,048 + +0.5 5,760,118 + PP_5054 0.18 -0.2 5,760,119 - PP_5054 0.18 +0.5 5,760,119 - PP_5054 0.18 -0.9 5,760,119 - PP_5054 0.18 +0.8 5,760,269 - PP_5054 0.77 -0.5 5,760,296 - PP_5054 0.88 +1.6 5,760,307 + -1.9 5,760,326 + -0.9 5,760,326 + -1.9 5,760,326 + -2.2 5,760,327 - -2.2 5,760,327 - -1.6 5,760,327 - -1.1 5,760,327 - +2.3
Or see this region's nucleotide sequence