Strain Fitness in Pseudomonas putida KT2440 around PP_5033

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
5,735,017	+		PP_5032	0.58	-0.8
5,735,018	-				+2.4
5,735,018	-		PP_5032	0.58	-0.1
5,735,018	-		PP_5032	0.58	-0.9
5,735,018	-		PP_5032	0.58	+0.0
5,735,018	-		PP_5032	0.58	+0.0
5,735,053	+		PP_5032	0.60	+0.9
5,735,170	+		PP_5032	0.68	-0.4
5,735,171	-		PP_5032	0.68	+2.6
5,735,207	+		PP_5032	0.70	+0.6
5,735,207	+		PP_5032	0.70	+0.3
5,735,207	+		PP_5032	0.70	+0.8
5,735,207	+		PP_5032	0.70	+0.7
5,735,208	-		PP_5032	0.70	+0.3
5,735,208	-		PP_5032	0.70	+0.6
5,735,208	-		PP_5032	0.70	-1.8
5,735,208	-		PP_5032	0.70	+1.3
5,735,208	-		PP_5032	0.70	-1.1
5,735,358	+		PP_5032	0.80	-1.3
5,735,511	-				-1.4
5,735,666	+				-1.4
5,735,666	+				+1.1
5,735,667	-				-0.7
5,735,667	-				+0.8
5,735,667	-				+0.1
5,735,707	+				-1.0
5,735,736	-				-0.8
5,735,736	-				-0.2
5,736,218	+		PP_5033	0.27	-1.9
5,736,218	+		PP_5033	0.27	-0.6
5,736,218	+		PP_5033	0.27	-1.6
5,736,218	+		PP_5033	0.27	-0.9
5,736,218	+		PP_5033	0.27	-1.7
5,736,218	+		PP_5033	0.27	-0.7
5,736,219	-		PP_5033	0.27	-0.6
5,736,219	-		PP_5033	0.27	+0.3
5,736,325	+		PP_5033	0.33	-0.3
5,736,325	+		PP_5033	0.33	-0.7
5,736,325	+		PP_5033	0.33	-0.3
5,736,326	-		PP_5033	0.33	-0.2
5,736,326	-		PP_5033	0.33	+0.3
5,736,326	-		PP_5033	0.33	-0.1
5,736,326	-		PP_5033	0.33	+0.4
5,736,401	-		PP_5033	0.38	-1.4
5,736,401	-		PP_5033	0.38	-0.9
5,736,760	+		PP_5033	0.59	+0.6
5,736,761	-		PP_5033	0.59	+0.5
5,736,761	-		PP_5033	0.59	-0.9
5,736,964	+		PP_5033	0.71	-1.5
5,736,964	+		PP_5033	0.71	+0.5
5,736,997	+		PP_5033	0.73	-0.4
5,736,997	+		PP_5033	0.73	-0.8
5,736,997	+		PP_5033	0.73	-0.6
5,736,997	+		PP_5033	0.73	+0.9
5,736,997	+		PP_5033	0.73	-1.5
5,736,998	-		PP_5033	0.73	+0.2
5,736,998	-		PP_5033	0.73	-0.7
5,737,030	+		PP_5033	0.75	-1.7
5,737,031	-		PP_5033	0.75	-0.8
5,737,031	-		PP_5033	0.75	-1.8
5,737,031	-		PP_5033	0.75	+3.6
5,737,060	+		PP_5033	0.77	-0.6
5,737,060	+		PP_5033	0.77	+0.1
5,737,060	+		PP_5033	0.77	-1.3
5,737,061	-		PP_5033	0.77	-0.0
5,737,061	-		PP_5033	0.77	-0.1
5,737,061	-		PP_5033	0.77	-0.1
5,737,252	+		PP_5033	0.88	+0.7
5,737,253	-		PP_5033	0.88	-0.5
5,737,253	-		PP_5033	0.88	-1.0
5,737,291	+				-0.3
5,737,292	-				-2.1
5,737,293	+				+1.3
5,737,293	+				+0.3
5,737,294	-				+1.4
5,737,294	-				-0.5
5,737,426	+				+0.5
5,737,427	-				-1.5
5,737,427	-				-0.4
5,737,493	+				-1.2
5,737,494	-				+0.3
5,737,494	-				-1.3
5,737,495	+				+0.3
5,737,495	+				-0.4
5,737,509	+				+1.4
5,737,541	-				-2.4
5,737,572	+				-0.5
5,737,573	-				-1.6
5,737,573	-				-0.2
5,737,585	-				-1.0
5,737,769	+		PP_5034	0.21	-0.5
5,737,769	+		PP_5034	0.21	+0.5
5,737,770	-		PP_5034	0.21	-0.2
5,737,826	-		PP_5034	0.31	-1.9
5,737,826	-		PP_5034	0.31	+3.3
5,737,826	-		PP_5034	0.31	+0.2
5,737,882	+		PP_5034	0.41	+0.7
5,737,882	+		PP_5034	0.41	+0.6
5,737,882	+		PP_5034	0.41	+1.1
5,737,882	+		PP_5034	0.41	-1.1
5,737,882	+		PP_5034	0.41	+0.3
5,737,882	+		PP_5034	0.41	-0.5
5,737,882	+		PP_5034	0.41	-0.5
5,737,883	-		PP_5034	0.41	+0.7
5,737,883	-		PP_5034	0.41	+0.3
5,737,883	-		PP_5034	0.41	-0.2
5,738,427	+		PP_5035	0.28	+0.7
5,738,427	+		PP_5035	0.28	+0.2
5,738,428	-		PP_5035	0.28	-2.1
5,738,428	-		PP_5035	0.28	-0.3
5,738,428	-		PP_5035	0.28	+0.8
5,738,428	-		PP_5035	0.28	-1.1
5,738,433	+		PP_5035	0.29	-0.6
5,738,433	+		PP_5035	0.29	-0.5
5,738,433	+		PP_5035	0.29	+0.8
5,738,434	-		PP_5035	0.29	-1.9
5,738,434	-		PP_5035	0.29	-1.9