Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5026 and PP_5027 are separated by 323 nucleotides PP_5027 and PP_5028 overlap by 4 nucleotides
PP_5026: PP_5026 - Glucans biosynthesis protein G, at 5,726,958 to 5,728,637
_5026
PP_5027: PP_5027 - D-tyrosyl-tRNA(Tyr) deacylase, at 5,728,961 to 5,729,398
_5027
PP_5028: PP_5028 - proline iminopeptidase, at 5,729,395 to 5,730,477
_5028
Position (kb) 5728
5729
5730 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5728.001 kb on - strand, within PP_5026 at 5728.001 kb on - strand, within PP_5026 at 5728.036 kb on + strand, within PP_5026 at 5728.037 kb on - strand, within PP_5026 at 5728.037 kb on - strand, within PP_5026 at 5728.037 kb on - strand, within PP_5026 at 5728.156 kb on + strand, within PP_5026 at 5728.243 kb on + strand, within PP_5026 at 5728.264 kb on + strand, within PP_5026 at 5728.330 kb on + strand, within PP_5026 at 5728.330 kb on + strand, within PP_5026 at 5728.330 kb on + strand, within PP_5026 at 5728.405 kb on + strand, within PP_5026 at 5728.405 kb on + strand, within PP_5026 at 5728.406 kb on - strand, within PP_5026 at 5728.406 kb on - strand, within PP_5026 at 5728.650 kb on + strand at 5728.688 kb on + strand at 5728.787 kb on - strand at 5728.839 kb on + strand at 5728.839 kb on + strand at 5728.840 kb on - strand at 5728.840 kb on - strand at 5728.909 kb on + strand at 5728.909 kb on + strand at 5728.909 kb on + strand at 5728.910 kb on - strand at 5728.910 kb on - strand at 5728.910 kb on - strand at 5728.910 kb on - strand at 5728.910 kb on - strand at 5728.913 kb on + strand at 5728.918 kb on + strand at 5728.964 kb on + strand at 5728.965 kb on - strand at 5728.965 kb on - strand at 5728.975 kb on + strand at 5728.975 kb on + strand at 5729.039 kb on + strand, within PP_5027 at 5729.077 kb on - strand, within PP_5027 at 5729.177 kb on + strand, within PP_5027 at 5729.576 kb on + strand, within PP_5028 at 5729.669 kb on + strand, within PP_5028 at 5729.882 kb on + strand, within PP_5028 at 5729.882 kb on + strand, within PP_5028 at 5729.882 kb on + strand, within PP_5028 at 5729.882 kb on + strand, within PP_5028 at 5729.882 kb on + strand, within PP_5028 at 5729.883 kb on - strand, within PP_5028 at 5729.894 kb on + strand, within PP_5028 at 5729.894 kb on + strand, within PP_5028 at 5729.927 kb on + strand, within PP_5028 at 5730.064 kb on + strand, within PP_5028 at 5730.064 kb on + strand, within PP_5028 at 5730.185 kb on + strand, within PP_5028 at 5730.203 kb on + strand, within PP_5028 at 5730.203 kb on + strand, within PP_5028 at 5730.293 kb on + strand, within PP_5028 at 5730.293 kb on + strand, within PP_5028 at 5730.294 kb on - strand, within PP_5028 at 5730.294 kb on - strand, within PP_5028 at 5730.387 kb on + strand at 5730.387 kb on + strand at 5730.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,728,001 - PP_5026 0.62 -0.3 5,728,001 - PP_5026 0.62 +1.3 5,728,036 + PP_5026 0.64 +0.1 5,728,037 - PP_5026 0.64 +1.3 5,728,037 - PP_5026 0.64 -0.3 5,728,037 - PP_5026 0.64 -0.9 5,728,156 + PP_5026 0.71 -0.5 5,728,243 + PP_5026 0.76 +0.0 5,728,264 + PP_5026 0.78 -1.3 5,728,330 + PP_5026 0.82 +0.9 5,728,330 + PP_5026 0.82 -0.8 5,728,330 + PP_5026 0.82 +0.5 5,728,405 + PP_5026 0.86 -0.8 5,728,405 + PP_5026 0.86 +0.6 5,728,406 - PP_5026 0.86 +0.1 5,728,406 - PP_5026 0.86 -0.6 5,728,650 + -0.8 5,728,688 + +0.2 5,728,787 - -1.9 5,728,839 + +1.0 5,728,839 + -0.4 5,728,840 - +1.1 5,728,840 - +0.5 5,728,909 + -1.8 5,728,909 + -0.0 5,728,909 + +0.5 5,728,910 - -0.1 5,728,910 - -1.4 5,728,910 - +1.8 5,728,910 - +0.4 5,728,910 - +0.3 5,728,913 + +2.6 5,728,918 + -0.4 5,728,964 + +1.2 5,728,965 - -0.6 5,728,965 - -0.9 5,728,975 + -1.4 5,728,975 + -1.8 5,729,039 + PP_5027 0.18 +0.1 5,729,077 - PP_5027 0.26 -1.1 5,729,177 + PP_5027 0.49 -2.3 5,729,576 + PP_5028 0.17 +0.2 5,729,669 + PP_5028 0.25 +1.4 5,729,882 + PP_5028 0.45 -0.6 5,729,882 + PP_5028 0.45 -0.3 5,729,882 + PP_5028 0.45 +0.8 5,729,882 + PP_5028 0.45 +1.1 5,729,882 + PP_5028 0.45 -0.0 5,729,883 - PP_5028 0.45 +2.6 5,729,894 + PP_5028 0.46 +0.8 5,729,894 + PP_5028 0.46 +1.3 5,729,927 + PP_5028 0.49 -1.3 5,730,064 + PP_5028 0.62 -0.1 5,730,064 + PP_5028 0.62 -1.3 5,730,185 + PP_5028 0.73 +0.6 5,730,203 + PP_5028 0.75 +1.2 5,730,203 + PP_5028 0.75 +1.0 5,730,293 + PP_5028 0.83 +0.1 5,730,293 + PP_5028 0.83 -1.2 5,730,294 - PP_5028 0.83 +0.2 5,730,294 - PP_5028 0.83 +0.5 5,730,387 + +0.1 5,730,387 + +0.9 5,730,387 + -0.7
Or see this region's nucleotide sequence