Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5021 and PP_5022 are separated by 109 nucleotides PP_5022 and PP_5023 overlap by 8 nucleotides PP_5023 and PP_5024 are separated by 79 nucleotides
PP_5021: PP_5021 - Methyl-accepting chemotaxis transducer, at 5,719,476 to 5,721,419
_5021
PP_5022: PP_5022 - Glutamine transport ATP-binding protein GlnQ, at 5,721,529 to 5,722,263
_5022
PP_5023: PP_5023 - Amino acid ABC transporter, permease protein, at 5,722,256 to 5,723,218
_5023
PP_5024: PP_5024 - Amino acid ABC transporter, periplasmic amino acid-binding protein, at 5,723,298 to 5,724,098
_5024
Position (kb)
5722
5723
5724 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.462 kb on - strand at 5721.462 kb on - strand at 5721.462 kb on - strand at 5721.530 kb on + strand at 5721.876 kb on + strand, within PP_5022 at 5721.877 kb on - strand, within PP_5022 at 5722.326 kb on + strand at 5722.326 kb on + strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.361 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023 at 5722.608 kb on + strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.609 kb on - strand, within PP_5023 at 5722.686 kb on + strand, within PP_5023 at 5722.686 kb on + strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.687 kb on - strand, within PP_5023 at 5722.738 kb on - strand, within PP_5023 at 5723.043 kb on + strand, within PP_5023 at 5723.077 kb on - strand, within PP_5023 at 5723.115 kb on + strand, within PP_5023 at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.139 kb on + strand at 5723.140 kb on - strand at 5723.140 kb on - strand at 5723.140 kb on - strand at 5723.225 kb on + strand at 5723.233 kb on + strand at 5723.234 kb on - strand at 5723.274 kb on - strand at 5723.274 kb on - strand at 5723.299 kb on + strand at 5723.300 kb on - strand at 5723.348 kb on + strand at 5723.356 kb on + strand at 5723.356 kb on + strand at 5723.356 kb on + strand at 5723.357 kb on - strand at 5723.357 kb on - strand at 5723.414 kb on - strand, within PP_5024 at 5723.449 kb on + strand, within PP_5024 at 5723.516 kb on - strand, within PP_5024 at 5723.527 kb on + strand, within PP_5024 at 5723.597 kb on - strand, within PP_5024 at 5723.665 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.689 kb on + strand, within PP_5024 at 5723.690 kb on - strand, within PP_5024 at 5723.854 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.968 kb on + strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5723.969 kb on - strand, within PP_5024 at 5724.086 kb on - strand at 5724.133 kb on + strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.134 kb on - strand at 5724.185 kb on + strand at 5724.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,721,461 + +0.5 5,721,461 + -1.7 5,721,461 + -0.3 5,721,461 + -1.4 5,721,462 - -1.7 5,721,462 - -0.3 5,721,462 - +0.6 5,721,530 + -1.0 5,721,876 + PP_5022 0.47 -2.5 5,721,877 - PP_5022 0.47 -1.8 5,722,326 + -0.3 5,722,326 + -0.9 5,722,327 - -0.6 5,722,327 - -0.1 5,722,327 - +2.2 5,722,361 - PP_5023 0.11 -0.4 5,722,379 - PP_5023 0.13 -0.7 5,722,379 - PP_5023 0.13 +0.4 5,722,608 + PP_5023 0.37 +0.6 5,722,609 - PP_5023 0.37 +1.3 5,722,609 - PP_5023 0.37 +0.5 5,722,609 - PP_5023 0.37 -0.9 5,722,609 - PP_5023 0.37 +1.1 5,722,609 - PP_5023 0.37 +0.6 5,722,686 + PP_5023 0.45 +1.0 5,722,686 + PP_5023 0.45 -0.8 5,722,687 - PP_5023 0.45 +0.3 5,722,687 - PP_5023 0.45 -0.2 5,722,687 - PP_5023 0.45 +0.3 5,722,738 - PP_5023 0.50 -2.0 5,723,043 + PP_5023 0.82 +2.7 5,723,077 - PP_5023 0.85 +1.3 5,723,115 + PP_5023 0.89 +3.2 5,723,139 + -2.2 5,723,139 + +0.8 5,723,139 + +1.2 5,723,139 + +0.6 5,723,139 + +0.8 5,723,139 + -0.3 5,723,140 - +0.3 5,723,140 - -0.1 5,723,140 - +1.5 5,723,225 + -0.6 5,723,233 + -0.3 5,723,234 - +1.0 5,723,274 - -0.1 5,723,274 - +0.3 5,723,299 + -1.2 5,723,300 - -0.3 5,723,348 + -2.2 5,723,356 + +2.2 5,723,356 + -0.3 5,723,356 + -0.7 5,723,357 - -0.1 5,723,357 - -0.5 5,723,414 - PP_5024 0.14 -0.4 5,723,449 + PP_5024 0.19 -1.2 5,723,516 - PP_5024 0.27 -2.5 5,723,527 + PP_5024 0.29 -0.2 5,723,597 - PP_5024 0.37 -0.5 5,723,665 + PP_5024 0.46 -1.6 5,723,689 + PP_5024 0.49 -2.1 5,723,689 + PP_5024 0.49 -1.4 5,723,689 + PP_5024 0.49 +0.2 5,723,690 - PP_5024 0.49 -0.8 5,723,854 + PP_5024 0.69 -0.3 5,723,968 + PP_5024 0.84 +0.9 5,723,968 + PP_5024 0.84 +0.1 5,723,968 + PP_5024 0.84 +0.6 5,723,969 - PP_5024 0.84 +1.1 5,723,969 - PP_5024 0.84 +1.4 5,723,969 - PP_5024 0.84 +1.1 5,724,086 - -2.3 5,724,133 + +0.5 5,724,134 - -0.6 5,724,134 - -0.6 5,724,134 - -0.5 5,724,134 - +1.2 5,724,185 + +1.7 5,724,185 + +0.9
Or see this region's nucleotide sequence