Strain Fitness in Pseudomonas putida KT2440 around PP_4974

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
5,665,049	+		PP_4973	0.21	-0.8
5,665,050	-		PP_4973	0.21	-0.3
5,665,111	+		PP_4973	0.29	+1.5
5,665,111	+		PP_4973	0.29	-0.4
5,665,111	+		PP_4973	0.29	-0.7
5,665,112	-		PP_4973	0.29	-0.3
5,665,133	-		PP_4973	0.31	-0.8
5,665,133	-		PP_4973	0.31	+0.3
5,665,187	+		PP_4973	0.38	-1.7
5,665,187	+		PP_4973	0.38	-0.9
5,665,238	+		PP_4973	0.44	+1.7
5,665,238	+		PP_4973	0.44	-2.0
5,665,239	-		PP_4973	0.44	+1.7
5,665,445	+		PP_4973	0.69	-1.3
5,665,445	+		PP_4973	0.69	-0.4
5,665,446	-		PP_4973	0.69	-2.4
5,665,446	-		PP_4973	0.69	+0.0
5,665,490	+		PP_4973	0.74	+0.2
5,665,491	-		PP_4973	0.74	+0.9
5,665,496	+		PP_4973	0.75	+0.6
5,665,497	-		PP_4973	0.75	+0.6
5,665,497	-		PP_4973	0.75	+0.0
5,665,497	-		PP_4973	0.75	+0.0
5,665,522	+		PP_4973	0.78	-1.4
5,665,650	+				-0.3
5,665,650	+				-2.0
5,665,740	+				-0.5
5,665,740	+				+0.9
5,665,740	+				+0.0
5,665,740	+				+0.1
5,665,741	-				-1.4
5,665,741	-				+0.4
5,665,741	-				+1.1
5,665,742	+				-1.0
5,665,742	+				-1.4
5,665,742	+				-0.8
5,665,742	+				-1.4
5,665,742	+				+0.5
5,665,743	-				+0.5
5,665,744	+				-1.2
5,665,744	+				+1.6
5,665,744	+				-1.0
5,665,744	+				+1.4
5,665,745	-				+1.0
5,665,801	+				-2.3
5,665,802	-				+0.8
5,665,802	-				+0.8
5,665,802	-				-0.3
5,665,850	+				-1.6
5,665,885	+				-1.5
5,665,974	+				-1.5
5,665,975	-				-0.8
5,665,975	-				+0.3
5,665,975	-				-0.1
5,665,976	+				-0.2
5,665,977	-				+0.4
5,665,977	-				+0.2
5,665,997	+				-1.4
5,665,997	+				-0.8
5,665,998	-				-1.7
5,666,036	-				-1.1
5,666,045	-				-0.1
5,666,045	-				+2.7
5,666,108	+				+0.5
5,666,108	+				-0.4
5,666,266	+		PP_4974	0.23	-1.2
5,666,292	+		PP_4974	0.25	-1.7
5,666,292	+		PP_4974	0.25	+0.6
5,666,293	-		PP_4974	0.25	-0.9
5,666,330	-		PP_4974	0.28	-0.1
5,666,380	+		PP_4974	0.32	-0.7
5,666,381	-		PP_4974	0.32	+0.2
5,666,446	-		PP_4974	0.37	-1.1
5,666,446	-		PP_4974	0.37	+0.0
5,666,446	-		PP_4974	0.37	+1.2
5,666,470	-		PP_4974	0.39	-0.3
5,666,523	-		PP_4974	0.43	-0.3
5,666,647	+		PP_4974	0.53	-2.6
5,666,709	-		PP_4974	0.58	-1.4
5,666,731	+		PP_4974	0.60	-1.2
5,666,732	-		PP_4974	0.60	-1.8
5,666,732	-		PP_4974	0.60	-2.6
5,666,831	-		PP_4974	0.68	+0.9
5,667,142	+				-1.0
5,667,142	+				-0.8
5,667,142	+				+0.2
5,667,142	+				-0.2
5,667,142	+				-0.1
5,667,142	+				+0.0
5,667,143	-				+0.4
5,667,143	-				-0.9
5,667,294	+				+1.4
5,667,330	+				+0.7
5,667,331	-				+0.7
5,667,434	-		PP_4975	0.35	-1.6
5,667,546	+		PP_4975	0.63	+0.3
5,667,546	+		PP_4975	0.63	+0.5
5,667,546	+		PP_4975	0.63	+0.4
5,667,546	+		PP_4975	0.63	-0.5
5,667,546	+		PP_4975	0.63	-2.1
5,667,546	+		PP_4975	0.63	-2.3
5,667,547	-		PP_4975	0.63	+0.2
5,667,588	+		PP_4975	0.73	-1.3
5,667,589	-		PP_4975	0.74	+0.4
5,667,699	-				-0.3
5,667,699	-				+0.3
5,667,724	+				-0.4
5,667,724	+				-1.0
5,667,724	+				-0.3
5,667,821	+				+1.0
5,667,821	+				-0.1
5,667,821	+				+0.4
5,667,821	+				-0.4
5,667,821	+				+1.0
5,667,822	-				+0.4
5,667,822	-				+2.8
5,667,822	-				+0.4
5,667,822	-				+1.2
5,667,823	+				+2.0
5,667,823	+				-1.9
5,667,823	+				-1.7
5,667,823	+				+0.9
5,667,838	+				+1.2
5,667,973	+				-1.3