Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4873 and PP_4874 are separated by 113 nucleotides PP_4874 and PP_4875 are separated by 20 nucleotides PP_4875 and PP_4876 are separated by 33 nucleotides
PP_4873: PP_4873 - replicative DNA helicase (ATPase), at 5,541,301 to 5,542,698
_4873
PP_4874: PP_4874 - 50S ribosomal protein L9, at 5,542,812 to 5,543,258
_4874
PP_4875: PP_4875 - conserved membrane protein of unknown function, at 5,543,279 to 5,544,142
_4875
PP_4876: PP_4876 - 30S ribosomal protein S18, at 5,544,176 to 5,544,406
_4876
Position (kb)
5542
5543
5544 Strain fitness (log2 ratio)
-1
0
1
2 at 5542.803 kb on + strand at 5542.804 kb on - strand at 5542.804 kb on - strand at 5542.813 kb on + strand at 5542.815 kb on - strand at 5542.821 kb on + strand at 5542.837 kb on - strand at 5542.837 kb on - strand at 5542.872 kb on + strand, within PP_4874 at 5542.873 kb on - strand, within PP_4874 at 5542.879 kb on + strand, within PP_4874 at 5543.195 kb on + strand, within PP_4874 at 5543.409 kb on + strand, within PP_4875 at 5543.409 kb on + strand, within PP_4875 at 5543.409 kb on + strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.410 kb on - strand, within PP_4875 at 5543.628 kb on + strand, within PP_4875 at 5543.647 kb on - strand, within PP_4875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,542,803 + +0.7 5,542,804 - +0.4 5,542,804 - -0.1 5,542,813 + -1.5 5,542,815 - -1.0 5,542,821 + -0.0 5,542,837 - -1.7 5,542,837 - +1.2 5,542,872 + PP_4874 0.13 -0.0 5,542,873 - PP_4874 0.14 -0.7 5,542,879 + PP_4874 0.15 +2.1 5,543,195 + PP_4874 0.86 -1.1 5,543,409 + PP_4875 0.15 +0.4 5,543,409 + PP_4875 0.15 -1.1 5,543,409 + PP_4875 0.15 +1.3 5,543,410 - PP_4875 0.15 -1.2 5,543,410 - PP_4875 0.15 +1.9 5,543,410 - PP_4875 0.15 +0.5 5,543,410 - PP_4875 0.15 -0.8 5,543,410 - PP_4875 0.15 +0.7 5,543,410 - PP_4875 0.15 -1.1 5,543,410 - PP_4875 0.15 +0.7 5,543,410 - PP_4875 0.15 +1.1 5,543,628 + PP_4875 0.40 -0.1 5,543,647 - PP_4875 0.43 -1.5
Or see this region's nucleotide sequence