Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4625 and PP_4626 overlap by 11 nucleotides PP_4626 and PP_4627 are separated by 97 nucleotides PP_4627 and PP_4628 are separated by 21 nucleotides PP_4628 and PP_4629 are separated by 115 nucleotides
PP_4625: PP_4625 - anti-holin regulator of holin CidA, at 5,249,998 to 5,250,684
_4625
PP_4626: PP_4626 - holin regulator of murein hydrolases, at 5,250,674 to 5,251,060
_4626
PP_4627: PP_4627 - transcriptional regulator, LysR family, at 5,251,158 to 5,252,039
_4627
PP_4628: PP_4628 - conserved protein of unknown function, at 5,252,061 to 5,252,516
_4628
PP_4629: PP_4629 - Sensory box protein, at 5,252,632 to 5,253,060
_4629
Position (kb) 5251
5252
5253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5250.162 kb on + strand, within PP_4625 at 5250.524 kb on + strand, within PP_4625 at 5250.594 kb on - strand, within PP_4625 at 5250.611 kb on + strand, within PP_4625 at 5250.612 kb on - strand, within PP_4625 at 5250.612 kb on - strand, within PP_4625 at 5250.632 kb on + strand at 5250.731 kb on + strand, within PP_4626 at 5250.731 kb on + strand, within PP_4626 at 5250.731 kb on + strand, within PP_4626 at 5250.732 kb on - strand, within PP_4626 at 5250.741 kb on - strand, within PP_4626 at 5250.741 kb on - strand, within PP_4626 at 5250.804 kb on + strand, within PP_4626 at 5250.914 kb on + strand, within PP_4626 at 5250.914 kb on + strand, within PP_4626 at 5250.914 kb on + strand, within PP_4626 at 5250.915 kb on - strand, within PP_4626 at 5250.915 kb on - strand, within PP_4626 at 5251.046 kb on + strand at 5251.046 kb on + strand at 5251.046 kb on + strand at 5251.047 kb on - strand at 5251.089 kb on + strand at 5251.435 kb on + strand, within PP_4627 at 5251.436 kb on - strand, within PP_4627 at 5251.436 kb on - strand, within PP_4627 at 5251.436 kb on - strand, within PP_4627 at 5251.624 kb on - strand, within PP_4627 at 5251.719 kb on - strand, within PP_4627 at 5251.734 kb on + strand, within PP_4627 at 5251.735 kb on - strand, within PP_4627 at 5251.965 kb on - strand at 5252.215 kb on + strand, within PP_4628 at 5252.216 kb on - strand, within PP_4628 at 5252.216 kb on - strand, within PP_4628 at 5252.219 kb on + strand, within PP_4628 at 5252.219 kb on + strand, within PP_4628 at 5252.227 kb on + strand, within PP_4628 at 5252.348 kb on + strand, within PP_4628 at 5252.486 kb on - strand at 5252.502 kb on - strand at 5252.725 kb on + strand, within PP_4629 at 5252.725 kb on + strand, within PP_4629 at 5252.746 kb on + strand, within PP_4629 at 5252.758 kb on + strand, within PP_4629 at 5252.759 kb on - strand, within PP_4629 at 5252.887 kb on + strand, within PP_4629 at 5252.888 kb on - strand, within PP_4629 at 5252.962 kb on + strand, within PP_4629 at 5252.962 kb on + strand, within PP_4629 at 5252.963 kb on - strand, within PP_4629
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,250,162 + PP_4625 0.24 +0.4 5,250,524 + PP_4625 0.77 -2.2 5,250,594 - PP_4625 0.87 +0.5 5,250,611 + PP_4625 0.89 -0.4 5,250,612 - PP_4625 0.89 +0.5 5,250,612 - PP_4625 0.89 -0.3 5,250,632 + -0.2 5,250,731 + PP_4626 0.15 -0.0 5,250,731 + PP_4626 0.15 +1.6 5,250,731 + PP_4626 0.15 -3.1 5,250,732 - PP_4626 0.15 -0.4 5,250,741 - PP_4626 0.17 +1.5 5,250,741 - PP_4626 0.17 -0.9 5,250,804 + PP_4626 0.34 -0.2 5,250,914 + PP_4626 0.62 -0.0 5,250,914 + PP_4626 0.62 -1.1 5,250,914 + PP_4626 0.62 +0.9 5,250,915 - PP_4626 0.62 -1.6 5,250,915 - PP_4626 0.62 +0.5 5,251,046 + +0.1 5,251,046 + +0.8 5,251,046 + +0.8 5,251,047 - -0.7 5,251,089 + +0.8 5,251,435 + PP_4627 0.31 +0.4 5,251,436 - PP_4627 0.32 -0.2 5,251,436 - PP_4627 0.32 -1.9 5,251,436 - PP_4627 0.32 -1.5 5,251,624 - PP_4627 0.53 +0.1 5,251,719 - PP_4627 0.64 -0.9 5,251,734 + PP_4627 0.65 -0.9 5,251,735 - PP_4627 0.65 -2.1 5,251,965 - +0.6 5,252,215 + PP_4628 0.34 +0.3 5,252,216 - PP_4628 0.34 -2.3 5,252,216 - PP_4628 0.34 -0.1 5,252,219 + PP_4628 0.35 +0.6 5,252,219 + PP_4628 0.35 -1.6 5,252,227 + PP_4628 0.36 -0.4 5,252,348 + PP_4628 0.63 +0.4 5,252,486 - -0.3 5,252,502 - +0.5 5,252,725 + PP_4629 0.22 -0.6 5,252,725 + PP_4629 0.22 -0.8 5,252,746 + PP_4629 0.27 +0.5 5,252,758 + PP_4629 0.29 +1.0 5,252,759 - PP_4629 0.30 -0.1 5,252,887 + PP_4629 0.59 +2.0 5,252,888 - PP_4629 0.60 +1.2 5,252,962 + PP_4629 0.77 -0.4 5,252,962 + PP_4629 0.77 +0.7 5,252,963 - PP_4629 0.77 -0.2
Or see this region's nucleotide sequence