Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4553 and PP_4554 are separated by 17 nucleotides PP_4554 and PP_4555 are separated by 21 nucleotides PP_4555 and PP_4556 are separated by 44 nucleotides
PP_4553: PP_4553 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,176,764 to 5,177,984
_4553
PP_4554: PP_4554 - conserved protein of unknown function, at 5,178,002 to 5,178,487
_4554
PP_4555: PP_4555 - conserved protein of unknown function, at 5,178,509 to 5,178,853
_4555
PP_4556: PP_4556 - conserved protein of unknown function, at 5,178,898 to 5,179,323
_4556
Position (kb) 5178
5179 Strain fitness (log2 ratio) -2
-1
0
1 at 5177.047 kb on + strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.129 kb on + strand, within PP_4553 at 5177.130 kb on - strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553 at 5177.211 kb on - strand, within PP_4553 at 5177.211 kb on - strand, within PP_4553 at 5177.408 kb on - strand, within PP_4553 at 5177.428 kb on + strand, within PP_4553 at 5177.428 kb on + strand, within PP_4553 at 5177.429 kb on - strand, within PP_4553 at 5177.481 kb on + strand, within PP_4553 at 5177.489 kb on - strand, within PP_4553 at 5177.493 kb on + strand, within PP_4553 at 5177.494 kb on - strand, within PP_4553 at 5177.531 kb on + strand, within PP_4553 at 5177.938 kb on - strand at 5178.411 kb on - strand, within PP_4554 at 5178.548 kb on - strand, within PP_4555 at 5178.548 kb on - strand, within PP_4555 at 5178.548 kb on - strand, within PP_4555 at 5178.618 kb on + strand, within PP_4555 at 5178.692 kb on + strand, within PP_4555 at 5178.831 kb on + strand at 5178.831 kb on + strand at 5178.832 kb on - strand at 5178.832 kb on - strand at 5178.832 kb on - strand at 5178.846 kb on + strand at 5178.846 kb on + strand at 5178.847 kb on - strand at 5178.847 kb on - strand at 5178.869 kb on - strand at 5178.967 kb on + strand, within PP_4556 at 5178.967 kb on + strand, within PP_4556 at 5178.968 kb on - strand, within PP_4556 at 5178.968 kb on - strand, within PP_4556 at 5179.167 kb on - strand, within PP_4556 at 5179.329 kb on + strand at 5179.329 kb on + strand at 5179.346 kb on - strand at 5179.346 kb on - strand at 5179.346 kb on - strand at 5179.346 kb on - strand at 5179.381 kb on + strand at 5179.382 kb on - strand at 5179.382 kb on - strand at 5179.382 kb on - strand at 5179.394 kb on + strand at 5179.424 kb on + strand at 5179.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,177,047 + PP_4553 0.23 -0.5 5,177,048 - PP_4553 0.23 +0.8 5,177,048 - PP_4553 0.23 +0.2 5,177,048 - PP_4553 0.23 -1.4 5,177,129 + PP_4553 0.30 -1.6 5,177,130 - PP_4553 0.30 +0.2 5,177,131 + PP_4553 0.30 +0.0 5,177,131 + PP_4553 0.30 -0.3 5,177,131 + PP_4553 0.30 +0.8 5,177,132 - PP_4553 0.30 +0.5 5,177,132 - PP_4553 0.30 -0.0 5,177,211 - PP_4553 0.37 -0.2 5,177,211 - PP_4553 0.37 -0.4 5,177,408 - PP_4553 0.53 +0.2 5,177,428 + PP_4553 0.54 -0.1 5,177,428 + PP_4553 0.54 -1.0 5,177,429 - PP_4553 0.54 +0.7 5,177,481 + PP_4553 0.59 -0.0 5,177,489 - PP_4553 0.59 +0.6 5,177,493 + PP_4553 0.60 -1.4 5,177,494 - PP_4553 0.60 -1.0 5,177,531 + PP_4553 0.63 +0.9 5,177,938 - -0.4 5,178,411 - PP_4554 0.84 -0.6 5,178,548 - PP_4555 0.11 -0.1 5,178,548 - PP_4555 0.11 -1.7 5,178,548 - PP_4555 0.11 -0.3 5,178,618 + PP_4555 0.32 -1.1 5,178,692 + PP_4555 0.53 -1.3 5,178,831 + -0.6 5,178,831 + +0.4 5,178,832 - +0.1 5,178,832 - -2.1 5,178,832 - +0.6 5,178,846 + +1.5 5,178,846 + -0.6 5,178,847 - +1.2 5,178,847 - +0.4 5,178,869 - -2.2 5,178,967 + PP_4556 0.16 -0.8 5,178,967 + PP_4556 0.16 -1.5 5,178,968 - PP_4556 0.16 +0.4 5,178,968 - PP_4556 0.16 -1.5 5,179,167 - PP_4556 0.63 -2.4 5,179,329 + +0.4 5,179,329 + -1.1 5,179,346 - -0.2 5,179,346 - -2.2 5,179,346 - +1.1 5,179,346 - -1.0 5,179,381 + -0.4 5,179,382 - -1.0 5,179,382 - -1.4 5,179,382 - -2.4 5,179,394 + -1.4 5,179,424 + -0.2 5,179,431 + -1.3
Or see this region's nucleotide sequence