Strain Fitness in Pseudomonas putida KT2440 around PP_4534

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
5,147,997	+		PP_4533	0.85	+1.0
5,148,061	-		PP_4533	0.89	+0.1
5,148,226	-				+0.4
5,148,271	+				-2.6
5,148,272	-				+0.9
5,148,272	-				+0.1
5,148,334	+		PP_5695	0.24	+0.5
5,148,334	+		PP_5695	0.24	-0.2
5,148,351	+		PP_5695	0.30	-2.5
5,148,351	+		PP_5695	0.30	-0.1
5,148,351	+		PP_5695	0.30	-0.4
5,148,400	+		PP_5695	0.48	+0.9
5,148,493	+		PP_5695	0.83	+0.8
5,148,493	+		PP_5695	0.83	-0.7
5,148,493	+		PP_5695	0.83	-0.1
5,148,494	-		PP_5695	0.83	+1.1
5,148,494	-		PP_5695	0.83	-1.4
5,148,547	+				+0.7
5,148,547	+				+1.8
5,148,547	+				-1.8
5,148,547	+				-0.1
5,148,548	-				-0.2
5,148,548	-				-0.7
5,148,548	-				+0.8
5,148,548	-				-1.6
5,148,548	-				-1.9
5,148,805	-		PP_4534	0.70	+0.8
5,148,805	-		PP_4534	0.70	-0.2
5,148,805	-		PP_4534	0.70	+0.3
5,148,849	+		PP_4534	0.88	+0.8
5,148,849	+		PP_4534	0.88	+0.4
5,148,850	-		PP_4534	0.88	+2.0
5,148,850	-		PP_4534	0.88	-1.3
5,148,882	+				-2.1
5,148,882	+				+0.6
5,148,883	-				+0.0
5,148,883	-				-0.5
5,148,907	-				-1.2
5,149,019	+				-1.1
5,149,020	-				+0.8
5,149,020	-				-1.5
5,149,020	-				+0.5
5,149,020	-				+0.3
5,149,020	-				-0.2
5,149,020	-				-0.5
5,149,025	+				+0.0
5,149,025	+				+2.8
5,149,025	+				+0.1
5,149,026	-				-0.1
5,149,026	-				+1.2
5,149,026	-				-0.4
5,149,026	-				-0.4
5,149,026	-				-0.0
5,149,026	-				+0.0
5,149,026	-				+0.4
5,149,026	-				+0.8
5,149,089	+				-1.6
5,149,090	-				-1.0
5,149,124	+				+1.1
5,149,124	+				-0.4
5,149,125	-				-0.1
5,149,125	-				+0.5
5,149,125	-				+0.4
5,149,142	+				-1.1
5,149,143	-				-0.3
5,149,194	+				+1.6
5,149,215	-				-2.0
5,149,253	+				-0.2
5,149,254	-				+0.5
5,149,261	-				+0.2
5,149,288	+				-0.0
5,149,288	+				-0.4
5,149,289	-				-0.9
5,149,289	-				+0.5
5,149,289	-				+1.8
5,149,289	-				+3.4
5,149,289	-				-0.6
5,149,289	-				-1.3
5,149,292	+				-0.1
5,149,292	+				-1.5
5,149,293	-				+0.3
5,149,293	-				-1.5
5,149,293	-				+0.2
5,149,348	+				+0.3
5,149,348	+				-0.0
5,149,349	-				-0.3
5,149,349	-				-0.9
5,149,351	+				+0.5
5,149,351	+				+0.1
5,149,351	+				+0.4
5,149,351	+				-1.1
5,149,352	-				+0.0
5,149,352	-				+2.0
5,149,352	-				-1.1
5,149,352	-				-0.6
5,149,355	+				+0.9
5,149,355	+				+0.6
5,149,356	-				-1.0
5,149,356	-				+2.3
5,149,356	-				+0.7
5,149,356	-				+0.3
5,149,356	-				-2.0
5,149,356	-				-1.3
5,149,356	-				+0.1
5,149,356	-				+0.3
5,149,356	-				-1.2
5,149,378	+				-0.6
5,149,378	+				-1.9
5,149,520	+				+0.5
5,149,520	+				-0.2
5,149,520	+				-0.4
5,149,520	+				+0.8
5,149,520	+				-0.8
5,149,520	+				+3.5
5,149,520	+				-0.1
5,149,521	-				-0.9
5,149,521	-				+0.1
5,149,521	-				-1.0
5,149,521	-				+1.1
5,149,521	-				-2.2
5,149,521	-				-1.2
5,149,620	+				-0.5
5,149,669	-		PP_4535	0.10	+1.8
5,149,687	+		PP_4535	0.11	-1.7
5,149,688	-		PP_4535	0.11	-0.8
5,149,688	-		PP_4535	0.11	+1.9
5,149,688	-		PP_4535	0.11	-1.5
5,149,723	-		PP_4535	0.13	-1.0
5,149,723	-		PP_4535	0.13	+0.4
5,149,733	+		PP_4535	0.14	-2.5
5,149,733	+		PP_4535	0.14	-1.3
5,149,733	+		PP_4535	0.14	-0.4
5,149,734	-				+1.0
5,149,734	-		PP_4535	0.14	+0.6
5,149,776	+		PP_4535	0.16	+0.6
5,149,776	+		PP_4535	0.16	+1.1
5,149,776	+		PP_4535	0.16	-0.5
5,149,776	+		PP_4535	0.16	+0.4
5,149,777	-		PP_4535	0.16	-0.4
5,149,777	-		PP_4535	0.16	+0.6
5,149,802	+		PP_4535	0.17	+0.9
5,149,835	+		PP_4535	0.19	-2.5
5,149,836	-		PP_4535	0.19	+1.9
5,149,836	-		PP_4535	0.19	+1.2