Strain Fitness in Pseudomonas putida KT2440 around PP_4521

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4519 and PP_4520 are separated by 62 nucleotidesPP_4520 and PP_4521 are separated by 64 nucleotidesPP_4521 and PP_4522 are separated by 138 nucleotides PP_4519: PP_4519 - Agglutination protein, at 5,132,919 to 5,134,277 _4519 PP_4520: PP_4520 - conserved protein of unknown function, at 5,134,340 to 5,134,939 _4520 PP_4521: PP_4521 - putative aerotaxis receptor, at 5,135,004 to 5,136,569 _4521 PP_4522: PP_4522 - Transcriptional regulator, LysR family, at 5,136,708 to 5,137,601 _4522 Position (kb) 5135 5136 5137Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 5134.009 kb on - strand, within PP_4519at 5134.108 kb on - strand, within PP_4519at 5134.205 kb on + strandat 5134.206 kb on - strandat 5134.206 kb on - strandat 5134.357 kb on + strandat 5134.357 kb on + strandat 5134.358 kb on - strandat 5134.358 kb on - strandat 5134.358 kb on - strandat 5134.373 kb on + strandat 5134.373 kb on + strandat 5134.374 kb on - strandat 5134.587 kb on + strand, within PP_4520at 5134.588 kb on - strand, within PP_4520at 5134.588 kb on - strand, within PP_4520at 5134.588 kb on - strand, within PP_4520at 5134.663 kb on - strand, within PP_4520at 5134.663 kb on - strand, within PP_4520at 5134.663 kb on - strand, within PP_4520at 5134.663 kb on - strand, within PP_4520at 5134.889 kb on + strandat 5134.890 kb on - strandat 5134.890 kb on - strandat 5135.031 kb on - strandat 5135.031 kb on - strandat 5135.038 kb on + strandat 5135.342 kb on + strand, within PP_4521at 5135.343 kb on - strand, within PP_4521at 5135.343 kb on - strand, within PP_4521at 5135.514 kb on - strand, within PP_4521at 5135.514 kb on - strand, within PP_4521at 5135.514 kb on - strand, within PP_4521at 5135.667 kb on - strand, within PP_4521at 5135.667 kb on - strand, within PP_4521at 5135.917 kb on + strand, within PP_4521at 5135.918 kb on - strand, within PP_4521at 5135.918 kb on - strand, within PP_4521at 5135.942 kb on + strand, within PP_4521at 5135.942 kb on + strand, within PP_4521at 5135.942 kb on + strand, within PP_4521at 5136.007 kb on + strand, within PP_4521at 5136.008 kb on - strand, within PP_4521at 5136.008 kb on - strand, within PP_4521at 5136.052 kb on + strand, within PP_4521at 5136.070 kb on + strand, within PP_4521at 5136.181 kb on + strand, within PP_4521at 5136.181 kb on + strand, within PP_4521at 5136.181 kb on + strand, within PP_4521at 5136.182 kb on - strand, within PP_4521at 5136.182 kb on - strand, within PP_4521at 5136.239 kb on - strand, within PP_4521at 5136.239 kb on - strand, within PP_4521at 5136.262 kb on + strand, within PP_4521at 5136.262 kb on + strand, within PP_4521at 5136.262 kb on + strand, within PP_4521at 5136.265 kb on - strand, within PP_4521at 5136.277 kb on + strand, within PP_4521at 5136.278 kb on - strand, within PP_4521at 5136.292 kb on + strand, within PP_4521at 5136.292 kb on + strand, within PP_4521at 5136.293 kb on - strand, within PP_4521at 5136.295 kb on + strand, within PP_4521at 5136.295 kb on + strand, within PP_4521at 5136.296 kb on - strand, within PP_4521at 5136.365 kb on + strand, within PP_4521at 5136.366 kb on - strand, within PP_4521at 5136.366 kb on - strand, within PP_4521at 5136.436 kb on + strandat 5136.437 kb on - strandat 5136.441 kb on - strandat 5136.475 kb on + strandat 5136.476 kb on - strandat 5136.476 kb on - strandat 5136.476 kb on - strandat 5136.476 kb on - strandat 5136.509 kb on + strandat 5136.595 kb on + strandat 5136.623 kb on + strandat 5136.623 kb on + strandat 5136.623 kb on + strandat 5136.624 kb on - strandat 5136.624 kb on - strandat 5136.627 kb on + strandat 5136.868 kb on + strand, within PP_4522at 5136.868 kb on + strand, within PP_4522at 5136.869 kb on - strand, within PP_4522at 5136.884 kb on - strand, within PP_4522at 5136.884 kb on - strand, within PP_4522at 5136.890 kb on + strand, within PP_4522at 5136.892 kb on + strand, within PP_4522at 5136.892 kb on + strand, within PP_4522at 5136.892 kb on + strand, within PP_4522at 5136.893 kb on - strand, within PP_4522at 5136.893 kb on - strand, within PP_4522at 5136.893 kb on - strand, within PP_4522at 5136.893 kb on - strand, within PP_4522at 5136.893 kb on - strand, within PP_4522at 5137.023 kb on + strand, within PP_4522at 5137.024 kb on - strand, within PP_4522at 5137.051 kb on + strand, within PP_4522at 5137.052 kb on - strand, within PP_4522at 5137.052 kb on - strand, within PP_4522at 5137.052 kb on - strand, within PP_4522at 5137.052 kb on - strand, within PP_4522at 5137.076 kb on - strand, within PP_4522at 5137.076 kb on - strand, within PP_4522at 5137.076 kb on - strand, within PP_4522at 5137.096 kb on + strand, within PP_4522at 5137.097 kb on - strand, within PP_4522at 5137.108 kb on + strand, within PP_4522at 5137.189 kb on + strand, within PP_4522at 5137.190 kb on - strand, within PP_4522at 5137.206 kb on + strand, within PP_4522at 5137.206 kb on + strand, within PP_4522at 5137.207 kb on - strand, within PP_4522at 5137.207 kb on - strand, within PP_4522at 5137.345 kb on + strand, within PP_4522at 5137.345 kb on + strand, within PP_4522at 5137.346 kb on - strand, within PP_4522at 5137.346 kb on - strand, within PP_4522at 5137.346 kb on - strand, within PP_4522at 5137.454 kb on + strand, within PP_4522at 5137.483 kb on + strand, within PP_4522at 5137.483 kb on + strand, within PP_4522at 5137.483 kb on + strand, within PP_4522at 5137.484 kb on - strand, within PP_4522at 5137.487 kb on + strand, within PP_4522at 5137.487 kb on + strand, within PP_4522at 5137.487 kb on + strand, within PP_4522at 5137.488 kb on - strand, within PP_4522at 5137.488 kb on - strand, within PP_4522at 5137.488 kb on - strand, within PP_4522at 5137.488 kb on - strand, within PP_4522at 5137.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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5,134,009 - PP_4519 0.80 +0.2
5,134,108 - PP_4519 0.87 -1.3
5,134,205 + -0.8
5,134,206 - -2.0
5,134,206 - +0.8
5,134,357 + +0.1
5,134,357 + +4.2
5,134,358 - +0.8
5,134,358 - +0.2
5,134,358 - -0.6
5,134,373 + -0.4
5,134,373 + +0.9
5,134,374 - -0.9
5,134,587 + PP_4520 0.41 -2.5
5,134,588 - PP_4520 0.41 -2.2
5,134,588 - PP_4520 0.41 +0.9
5,134,588 - PP_4520 0.41 -0.1
5,134,663 - PP_4520 0.54 +1.0
5,134,663 - PP_4520 0.54 -1.1
5,134,663 - PP_4520 0.54 -1.2
5,134,663 - PP_4520 0.54 +2.2
5,134,889 + +0.0
5,134,890 - +1.9
5,134,890 - +0.2
5,135,031 - +0.6
5,135,031 - +1.0
5,135,038 + -1.4
5,135,342 + PP_4521 0.22 -1.1
5,135,343 - PP_4521 0.22 -0.7
5,135,343 - PP_4521 0.22 +0.8
5,135,514 - PP_4521 0.33 -0.7
5,135,514 - PP_4521 0.33 -1.7
5,135,514 - PP_4521 0.33 -1.7
5,135,667 - PP_4521 0.42 -1.3
5,135,667 - PP_4521 0.42 -0.9
5,135,917 + PP_4521 0.58 -0.7
5,135,918 - PP_4521 0.58 -1.1
5,135,918 - PP_4521 0.58 -2.1
5,135,942 + PP_4521 0.60 -1.0
5,135,942 + PP_4521 0.60 -0.5
5,135,942 + PP_4521 0.60 -0.9
5,136,007 + PP_4521 0.64 -1.4
5,136,008 - PP_4521 0.64 -0.9
5,136,008 - PP_4521 0.64 +0.1
5,136,052 + PP_4521 0.67 -0.3
5,136,070 + PP_4521 0.68 +0.7
5,136,181 + PP_4521 0.75 +0.2
5,136,181 + PP_4521 0.75 -1.1
5,136,181 + PP_4521 0.75 -0.3
5,136,182 - PP_4521 0.75 +0.1
5,136,182 - PP_4521 0.75 +0.0
5,136,239 - PP_4521 0.79 -0.0
5,136,239 - PP_4521 0.79 -0.3
5,136,262 + PP_4521 0.80 +0.6
5,136,262 + PP_4521 0.80 -1.2
5,136,262 + PP_4521 0.80 -0.2
5,136,265 - PP_4521 0.81 +1.5
5,136,277 + PP_4521 0.81 -0.6
5,136,278 - PP_4521 0.81 -0.4
5,136,292 + PP_4521 0.82 -0.3
5,136,292 + PP_4521 0.82 -3.3
5,136,293 - PP_4521 0.82 -1.2
5,136,295 + PP_4521 0.82 -0.6
5,136,295 + PP_4521 0.82 +0.9
5,136,296 - PP_4521 0.83 +0.3
5,136,365 + PP_4521 0.87 -0.4
5,136,366 - PP_4521 0.87 -2.4
5,136,366 - PP_4521 0.87 -0.5
5,136,436 + -1.0
5,136,437 - +0.5
5,136,441 - -1.4
5,136,475 + -0.8
5,136,476 - +1.9
5,136,476 - -2.6
5,136,476 - -2.0
5,136,476 - +0.8
5,136,509 + +0.3
5,136,595 + -0.2
5,136,623 + -1.8
5,136,623 + -0.2
5,136,623 + -1.9
5,136,624 - -1.5
5,136,624 - +0.9
5,136,627 + -1.2
5,136,868 + PP_4522 0.18 +1.1
5,136,868 + PP_4522 0.18 -0.6
5,136,869 - PP_4522 0.18 -0.4
5,136,884 - PP_4522 0.20 -2.0
5,136,884 - PP_4522 0.20 +0.0
5,136,890 + PP_4522 0.20 -1.8
5,136,892 + PP_4522 0.21 -0.0
5,136,892 + PP_4522 0.21 +2.2
5,136,892 + PP_4522 0.21 -1.1
5,136,893 - PP_4522 0.21 -1.6
5,136,893 - PP_4522 0.21 -1.2
5,136,893 - PP_4522 0.21 -0.1
5,136,893 - PP_4522 0.21 +1.1
5,136,893 - PP_4522 0.21 -1.2
5,137,023 + PP_4522 0.35 +0.8
5,137,024 - PP_4522 0.35 +1.1
5,137,051 + PP_4522 0.38 -1.4
5,137,052 - PP_4522 0.38 -0.2
5,137,052 - PP_4522 0.38 -0.9
5,137,052 - PP_4522 0.38 -1.0
5,137,052 - PP_4522 0.38 +0.7
5,137,076 - PP_4522 0.41 -1.5
5,137,076 - PP_4522 0.41 +0.6
5,137,076 - PP_4522 0.41 +0.9
5,137,096 + PP_4522 0.43 -1.4
5,137,097 - PP_4522 0.44 -1.7
5,137,108 + PP_4522 0.45 +0.7
5,137,189 + PP_4522 0.54 +0.4
5,137,190 - PP_4522 0.54 -0.5
5,137,206 + PP_4522 0.56 +0.6
5,137,206 + PP_4522 0.56 -0.6
5,137,207 - PP_4522 0.56 -0.8
5,137,207 - PP_4522 0.56 -1.3
5,137,345 + PP_4522 0.71 -0.8
5,137,345 + PP_4522 0.71 +0.2
5,137,346 - PP_4522 0.71 -0.3
5,137,346 - PP_4522 0.71 +1.0
5,137,346 - PP_4522 0.71 +0.1
5,137,454 + PP_4522 0.83 -0.3
5,137,483 + PP_4522 0.87 -1.5
5,137,483 + PP_4522 0.87 -1.3
5,137,483 + PP_4522 0.87 +0.5
5,137,484 - PP_4522 0.87 +0.4
5,137,487 + PP_4522 0.87 -0.4
5,137,487 + PP_4522 0.87 +0.4
5,137,487 + PP_4522 0.87 +0.3
5,137,488 - PP_4522 0.87 +1.4
5,137,488 - PP_4522 0.87 -0.9
5,137,488 - PP_4522 0.87 -0.1
5,137,488 - PP_4522 0.87 -1.4
5,137,562 - +0.2

Or see this region's nucleotide sequence