Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4501 and PP_4502 overlap by 32 nucleotides PP_4502 and PP_4503 are separated by 164 nucleotides PP_4503 and PP_4504 are separated by 162 nucleotides PP_4504 and PP_4505 are separated by 58 nucleotides
PP_4501: PP_4501 - Probable intracellular septation protein A, at 5,115,853 to 5,116,446
_4501
PP_4502: PP_4502 - putative enzyme, at 5,116,415 to 5,116,747
_4502
PP_4503: PP_4503 - DNA-binding response regulator, at 5,116,912 to 5,117,589
_4503
PP_4504: PP_4504 - conserved exported protein of unknown function, at 5,117,752 to 5,118,168
_4504
PP_4505: PP_4505 - Sensor histidine kinase, at 5,118,227 to 5,119,567
_4505
Position (kb) 5116
5117
5118 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5116.425 kb on + strand at 5116.425 kb on + strand at 5116.426 kb on - strand at 5116.426 kb on - strand at 5116.454 kb on + strand, within PP_4502 at 5116.621 kb on + strand, within PP_4502 at 5116.685 kb on + strand, within PP_4502 at 5116.685 kb on + strand at 5116.686 kb on - strand, within PP_4502 at 5116.686 kb on - strand, within PP_4502 at 5116.686 kb on - strand, within PP_4502 at 5116.703 kb on + strand, within PP_4502 at 5116.737 kb on + strand at 5116.738 kb on - strand at 5116.738 kb on - strand at 5116.774 kb on - strand at 5116.832 kb on + strand at 5116.832 kb on + strand at 5116.832 kb on + strand at 5116.833 kb on - strand at 5116.833 kb on - strand at 5116.835 kb on - strand at 5116.835 kb on - strand at 5117.060 kb on + strand, within PP_4503 at 5117.259 kb on - strand, within PP_4503 at 5117.318 kb on - strand, within PP_4503 at 5117.473 kb on + strand, within PP_4503 at 5117.474 kb on - strand, within PP_4503 at 5117.549 kb on + strand at 5117.550 kb on - strand at 5117.550 kb on - strand at 5117.572 kb on + strand at 5117.573 kb on - strand at 5117.575 kb on + strand at 5117.575 kb on + strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.576 kb on - strand at 5117.578 kb on + strand at 5117.579 kb on - strand at 5117.579 kb on - strand at 5117.587 kb on + strand at 5117.587 kb on + strand at 5117.658 kb on + strand at 5117.658 kb on + strand at 5117.659 kb on - strand at 5117.669 kb on + strand at 5117.670 kb on - strand at 5117.960 kb on - strand, within PP_4504 at 5117.960 kb on - strand, within PP_4504 at 5118.166 kb on + strand at 5118.166 kb on + strand at 5118.167 kb on - strand at 5118.167 kb on - strand at 5118.167 kb on - strand at 5118.221 kb on + strand at 5118.222 kb on - strand at 5118.393 kb on - strand, within PP_4505 at 5118.587 kb on + strand, within PP_4505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,116,425 + -0.3 5,116,425 + +1.9 5,116,426 - +0.4 5,116,426 - -0.6 5,116,454 + PP_4502 0.12 +0.2 5,116,621 + PP_4502 0.62 -1.8 5,116,685 + PP_4502 0.81 +1.1 5,116,685 + +1.8 5,116,686 - PP_4502 0.81 +0.1 5,116,686 - PP_4502 0.81 +0.1 5,116,686 - PP_4502 0.81 -2.0 5,116,703 + PP_4502 0.86 -0.0 5,116,737 + +1.9 5,116,738 - -0.2 5,116,738 - +1.1 5,116,774 - +0.6 5,116,832 + -0.9 5,116,832 + -0.3 5,116,832 + -0.3 5,116,833 - +0.2 5,116,833 - +0.5 5,116,835 - -0.2 5,116,835 - +0.0 5,117,060 + PP_4503 0.22 -2.0 5,117,259 - PP_4503 0.51 -1.4 5,117,318 - PP_4503 0.60 +0.2 5,117,473 + PP_4503 0.83 -1.0 5,117,474 - PP_4503 0.83 -1.7 5,117,549 + +0.8 5,117,550 - -2.3 5,117,550 - -0.3 5,117,572 + +0.2 5,117,573 - +0.1 5,117,575 + +0.1 5,117,575 + -0.1 5,117,576 - +1.3 5,117,576 - -1.2 5,117,576 - -0.6 5,117,576 - -1.8 5,117,576 - -0.6 5,117,576 - -0.5 5,117,578 + +0.4 5,117,579 - +0.3 5,117,579 - -1.6 5,117,587 + -0.5 5,117,587 + -0.9 5,117,658 + +0.2 5,117,658 + +0.7 5,117,659 - +0.7 5,117,669 + -0.4 5,117,670 - +0.9 5,117,960 - PP_4504 0.50 -0.3 5,117,960 - PP_4504 0.50 -1.8 5,118,166 + +0.5 5,118,166 + -2.2 5,118,167 - -1.4 5,118,167 - -0.5 5,118,167 - -0.1 5,118,221 + +0.2 5,118,222 - -0.7 5,118,393 - PP_4505 0.12 -1.8 5,118,587 + PP_4505 0.27 +0.0
Or see this region's nucleotide sequence