Strain Fitness in Pseudomonas putida KT2440 around PP_4465

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
5,066,976	+		PP_4464	0.38	+0.4
5,067,096	+		PP_4464	0.51	-2.3
5,067,096	+		PP_4464	0.51	+1.7
5,067,146	+		PP_4464	0.57	-0.5
5,067,146	+		PP_4464	0.57	+1.3
5,067,253	-		PP_4464	0.69	-1.3
5,067,340	-		PP_4464	0.78	+0.7
5,067,431	-		PP_4464	0.88	-0.7
5,067,513	+				-0.7
5,067,531	-				+0.7
5,067,563	+				+0.6
5,067,587	-				-1.3
5,067,594	+				+0.2
5,067,610	+				+0.5
5,067,610	+				-1.6
5,067,610	+				+1.0
5,067,611	-				-1.4
5,067,611	-				+1.7
5,067,824	-				-1.6
5,067,907	+				-1.6
5,067,921	-				-1.6
5,067,972	-				+0.4
5,068,140	+		PP_4465	0.14	-0.7
5,068,171	+		PP_4465	0.16	+0.1
5,068,172	-		PP_4465	0.16	+0.2
5,068,172	-		PP_4465	0.16	-1.9
5,068,175	+		PP_4465	0.16	+0.4
5,068,175	+		PP_4465	0.16	+1.5
5,068,175	+		PP_4465	0.16	-1.7
5,068,175	+		PP_4465	0.16	-0.5
5,068,175	+		PP_4465	0.16	+0.8
5,068,207	+		PP_4465	0.19	+1.3
5,068,207	+		PP_4465	0.19	+0.1
5,068,208	-		PP_4465	0.19	-0.5
5,068,289	+		PP_4465	0.25	-1.4
5,068,291	+		PP_4465	0.25	-0.8
5,068,291	+		PP_4465	0.25	+0.5
5,068,291	+		PP_4465	0.25	-0.2
5,068,292	-		PP_4465	0.25	-2.1
5,068,292	-		PP_4465	0.25	-1.0
5,068,292	-		PP_4465	0.25	+2.1
5,068,292	-		PP_4465	0.25	-0.9
5,068,295	+		PP_4465	0.25	-2.3
5,068,297	+		PP_4465	0.25	-1.1
5,068,297	+		PP_4465	0.25	-0.6
5,068,353	+		PP_4465	0.30	-2.8
5,068,353	+		PP_4465	0.30	-1.6
5,068,762	-		PP_4465	0.60	-2.1
5,068,809	+		PP_4465	0.63	-2.0
5,068,812	-		PP_4465	0.64	-0.1
5,069,028	+		PP_4465	0.80	+1.2
5,069,028	+		PP_4465	0.80	-1.2
5,069,043	+		PP_4465	0.81	-0.7
5,069,119	+		PP_4465	0.86	-1.1
5,069,119	+		PP_4465	0.86	-1.3
5,069,177	+				-0.6
5,069,177	+				+0.2
5,069,180	+				-0.9
5,069,181	-				-2.1
5,069,181	-				-1.5
5,069,208	+				+0.5
5,069,326	+				-1.8
5,069,427	+				-1.2
5,069,507	+				-0.7
5,069,544	-				+1.2
5,069,777	+				+0.0
5,069,800	-				-1.3
5,069,820	+				+0.6
5,069,884	-				-1.3
5,069,898	-				-0.7
5,070,003	+				-0.3
5,070,015	+				-0.0
5,070,016	-				+0.4
5,070,041	-				+0.5
5,070,056	-				-0.4
5,070,115	+		PP_4466	0.12	-1.3
5,070,115	+		PP_4466	0.12	-0.5
5,070,185	+		PP_4466	0.20	+0.4
5,070,197	-		PP_4466	0.21	+0.2
5,070,209	-		PP_4466	0.22	-0.6
5,070,209	-		PP_4466	0.22	+0.9
5,070,218	+		PP_4466	0.23	-0.3
5,070,219	-		PP_4466	0.23	-1.7
5,070,242	+		PP_4466	0.26	+0.6
5,070,243	-		PP_4466	0.26	+0.3
5,070,243	-		PP_4466	0.26	-0.5
5,070,243	-		PP_4466	0.26	-1.0
5,070,243	-		PP_4466	0.26	+0.2
5,070,243	-		PP_4466	0.26	-0.4
5,070,262	-		PP_4466	0.28	-0.1
5,070,291	-		PP_4466	0.32	-2.1
5,070,291	-		PP_4466	0.32	+0.3