Strain Fitness in Pseudomonas putida KT2440 around PP_4264

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,847,716	+				+0.3
4,847,716	+				+0.6
4,847,716	+				+2.8
4,847,716	+				+0.3
4,847,716	+				-2.1
4,847,716	+				-0.1
4,847,717	-				+1.2
4,847,761	+		PP_4262	0.26	+0.7
4,847,761	+		PP_4262	0.26	-1.5
4,847,761	+		PP_4262	0.26	-0.2
4,847,762	-		PP_4262	0.27	+1.3
4,847,762	-		PP_4262	0.27	+2.1
4,847,762	-		PP_4262	0.27	+1.8
4,848,010	-		PP_4263	0.14	-0.4
4,848,089	+		PP_4263	0.26	-1.5
4,848,201	-		PP_4263	0.42	-1.2
4,848,266	-		PP_4263	0.52	+1.3
4,848,266	-		PP_4263	0.52	+1.5
4,848,362	+		PP_4263	0.66	-0.2
4,848,362	+		PP_4263	0.66	-1.2
4,848,362	+		PP_4263	0.66	+1.6
4,848,363	-		PP_4263	0.66	+0.4
4,848,540	+				+0.2
4,848,540	+				+1.5
4,848,541	-				+0.7
4,848,541	-				+0.1
4,848,695	-				-1.9
4,848,764	+				+2.5
4,848,869	+		PP_4264	0.12	-0.3
4,848,870	-		PP_4264	0.12	-0.9
4,848,902	+		PP_4264	0.15	-0.7
4,848,905	+		PP_4264	0.15	-1.1
4,848,905	+		PP_4264	0.15	-1.3
4,848,906	-		PP_4264	0.15	-0.4
4,848,906	-		PP_4264	0.15	+1.4
4,848,906	-		PP_4264	0.15	-2.3
4,849,009	+		PP_4264	0.22	+0.1
4,849,137	-		PP_4264	0.32	-0.4
4,849,224	+		PP_4264	0.38	+0.6
4,849,224	+		PP_4264	0.38	-1.6
4,849,225	-		PP_4264	0.38	+0.3
4,849,225	-		PP_4264	0.38	+1.6
4,849,225	-		PP_4264	0.38	+0.3
4,849,225	-		PP_4264	0.38	-1.1
4,849,346	+		PP_4264	0.47	-1.0
4,849,347	-		PP_4264	0.47	+2.0
4,849,347	-		PP_4264	0.47	-0.6
4,849,347	-		PP_4264	0.47	+0.0
4,849,347	-		PP_4264	0.47	+1.5
4,849,347	-		PP_4264	0.47	-1.7
4,849,436	+		PP_4264	0.53	-1.4
4,849,436	+		PP_4264	0.53	+0.0
4,849,436	+		PP_4264	0.53	-1.2
4,849,437	-		PP_4264	0.53	-1.7
4,849,437	-		PP_4264	0.53	-2.1
4,849,437	-		PP_4264	0.53	-0.3
4,849,719	-		PP_4264	0.74	+1.5
4,849,748	+		PP_4264	0.76	-1.1
4,849,819	-		PP_4264	0.81	+0.5
4,849,819	-		PP_4264	0.81	-0.5
4,850,020	-				+0.1
4,850,020	-				+0.3
4,850,131	+				-0.7
4,850,131	+				+0.4
4,850,131	+				+0.2
4,850,131	+				-0.0
4,850,131	+				-0.5
4,850,132	-				+1.3
4,850,132	-				-1.2
4,850,132	-				+0.6
4,850,208	-				+0.6
4,850,226	+				+1.9
4,850,226	+				-1.8
4,850,227	-				-0.6
4,850,232	+				-1.4
4,850,233	-				+0.1
4,850,239	+				-0.6
4,850,239	+				+0.5
4,850,239	+				-1.2
4,850,459	+		PP_4265	0.26	-0.4
4,850,466	+		PP_4265	0.27	+0.6
4,850,670	+		PP_4265	0.54	+0.6
4,850,671	-		PP_4265	0.55	-1.1
4,850,671	-		PP_4265	0.55	-1.1
4,850,861	+		PP_4265	0.80	+2.3
4,850,861	+		PP_4265	0.80	-0.3
4,850,861	+		PP_4265	0.80	+0.0
4,850,862	-		PP_4265	0.81	-0.7
4,850,877	+		PP_4265	0.83	-0.3
4,851,015	+				+0.1
4,851,024	+				+1.8
4,851,024	+				+0.8
4,851,024	+				+0.0
4,851,025	-				-0.6
4,851,025	-				+0.0