Strain Fitness in Pseudomonas putida KT2440 around PP_4253

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,837,254	+		PP_4250	0.88	-1.5
4,837,255	-		PP_4250	0.88	+1.5
4,837,484	+				+0.1
4,837,485	-				-0.9
4,837,485	-				-1.7
4,837,606	+		PP_4251	0.29	+0.2
4,837,607	-		PP_4251	0.29	+1.6
4,837,804	+		PP_4251	0.61	+1.7
4,837,804	+		PP_4251	0.61	+1.9
4,837,804	+		PP_4251	0.61	-0.9
4,837,805	-		PP_4251	0.61	+0.5
4,837,846	+		PP_4251	0.68	-0.6
4,837,927	+		PP_4251	0.81	+1.2
4,837,999	+				+0.2
4,838,000	-				+2.2
4,838,228	+				-0.2
4,838,254	+				+0.4
4,838,254	+				-0.9
4,838,255	-				-0.0
4,838,258	+				-0.1
4,838,258	+				+0.5
4,838,258	+				+1.3
4,838,258	+				+1.4
4,838,259	-				+0.4
4,838,259	-				-0.4
4,838,268	+				+1.1
4,838,268	+				-2.4
4,838,268	+				-0.2
4,838,376	+		PP_4253	0.15	-1.2
4,838,377	-		PP_4253	0.15	-1.5
4,838,384	+		PP_4253	0.16	-1.5
4,838,384	+		PP_4253	0.16	+1.1
4,838,451	-				+2.3
4,838,463	-		PP_4253	0.24	+1.1
4,839,010	+		PP_4253	0.82	-1.1
4,839,014	+		PP_4253	0.82	-1.0
4,839,125	+				-0.6
4,839,125	+				+1.7
4,839,125	+				-1.1
4,839,125	+				-1.0
4,839,125	+				-0.5
4,839,126	-				+0.7
4,839,126	-				+1.4
4,839,126	-				+1.0
4,839,126	-				-0.1
4,839,131	+				+0.0
4,839,131	+				+2.1
4,839,131	+				+0.2
4,839,131	+				-1.4
4,839,131	+				+3.9
4,839,131	+				+0.9
4,839,131	+				-0.6
4,839,132	-				+1.0
4,839,132	-				+2.6
4,839,132	-				-1.0
4,839,132	-				-1.4
4,839,132	-				+2.7
4,839,132	-				+0.3
4,839,134	-				-0.2
4,839,134	-				+0.6
4,839,134	-				+0.3
4,839,296	+				+0.1
4,839,342	+				+0.9
4,839,342	+				-0.7
4,839,342	+				-1.5
4,839,342	+				-1.5
4,839,342	+				-0.3
4,839,343	-				-0.0
4,839,343	-				+0.8
4,839,343	-				-0.5
4,839,343	-				-0.6
4,839,343	-				+1.1
4,839,545	-		PP_4254	0.68	-0.8
4,839,564	-		PP_4254	0.75	-0.1
4,839,662	+				-0.6
4,839,662	+				-0.6
4,839,663	-				-0.1
4,839,663	-				+0.3
4,839,664	+				+1.3
4,839,664	+				-0.5
4,839,664	+				-1.7
4,839,664	+				+1.1
4,839,665	-				-1.1
4,839,665	-				-0.5
4,839,668	+				-0.8
4,839,668	+				-0.7
4,839,668	+				+0.2
4,839,668	+				-2.4
4,839,668	+				-1.4
4,839,669	-				-1.4
4,839,669	-				+1.7
4,839,669	-				+1.7
4,839,669	-				+0.3
4,839,669	-				+1.0
4,839,669	-				-2.1
4,839,669	-				-0.0
4,839,670	+				-1.8
4,839,670	+				+0.5
4,839,670	+				-0.4
4,839,670	+				-1.2
4,839,670	+				+1.8
4,839,671	-				-0.8
4,839,671	-				-1.9
4,839,869	+		PP_4255	0.16	-0.3
4,839,869	+		PP_4255	0.16	+0.7
4,839,869	+		PP_4255	0.16	-0.4
4,839,870	-		PP_4255	0.16	-2.3
4,839,870	-		PP_4255	0.16	-0.7
4,839,870	-		PP_4255	0.16	+1.3
4,839,870	-		PP_4255	0.16	-0.5
4,839,872	+		PP_4255	0.16	+0.9
4,839,872	+		PP_4255	0.16	+0.5
4,839,872	+		PP_4255	0.16	+1.2
4,839,872	+		PP_4255	0.16	-0.8
4,839,872	+		PP_4255	0.16	-0.9
4,839,872	+		PP_4255	0.16	+0.7
4,839,872	+		PP_4255	0.16	+0.1
4,839,873	-		PP_4255	0.16	-0.5
4,839,873	-		PP_4255	0.16	-0.2
4,839,873	-		PP_4255	0.16	+1.1
4,839,873	-		PP_4255	0.16	-0.2
4,839,873	-		PP_4255	0.16	-0.7
4,839,986	+		PP_4255	0.24	+0.2
4,839,987	-		PP_4255	0.24	+1.6
4,840,005	-		PP_4255	0.26	+0.9
4,840,055	-		PP_4255	0.29	-0.7
4,840,058	+		PP_4255	0.29	+1.7
4,840,059	-		PP_4255	0.29	+0.3
4,840,081	+		PP_4255	0.31	+0.8
4,840,109	+		PP_4255	0.33	+1.0
4,840,109	+		PP_4255	0.33	+0.7
4,840,109	+		PP_4255	0.33	-0.2
4,840,109	+		PP_4255	0.33	+1.1
4,840,109	+		PP_4255	0.33	+1.0
4,840,109	+		PP_4255	0.33	-0.1
4,840,109	+		PP_4255	0.33	-0.7
4,840,109	+		PP_4255	0.33	+0.9
4,840,109	+		PP_4255	0.33	+0.9
4,840,110	-		PP_4255	0.33	+0.5
4,840,110	-		PP_4255	0.33	+1.2
4,840,110	-		PP_4255	0.33	+0.7
4,840,110	-		PP_4255	0.33	-0.6
4,840,110	-		PP_4255	0.33	-1.5
4,840,110	-		PP_4255	0.33	-0.7
4,840,110	-		PP_4255	0.33	-0.7
4,840,110	-		PP_4255	0.33	-2.0
4,840,127	+		PP_4255	0.34	-0.5
4,840,127	+		PP_4255	0.34	-0.8