Strain Fitness in Pseudomonas putida KT2440 around PP_4232

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,802,547	+		PP_4231	0.21	-1.3
4,802,548	-		PP_4231	0.21	+2.1
4,802,548	-		PP_4231	0.21	+0.2
4,802,548	-		PP_4231	0.21	-1.6
4,802,684	+		PP_4231	0.35	-0.4
4,802,685	-		PP_4231	0.35	-0.0
4,802,685	-		PP_4231	0.35	+2.0
4,802,711	+		PP_4231	0.38	+0.4
4,802,712	-		PP_4231	0.38	+0.5
4,802,875	+		PP_4231	0.55	+0.7
4,802,876	-		PP_4231	0.55	+1.0
4,802,876	-		PP_4231	0.55	+0.8
4,802,876	-		PP_4231	0.55	-2.2
4,802,876	-		PP_4231	0.55	+0.5
4,803,222	+				+1.0
4,803,223	-				-0.2
4,803,223	-				-1.3
4,803,223	-				-1.0
4,803,296	+				-0.8
4,803,296	+				-1.7
4,803,297	-				-0.4
4,803,297	-				-2.1
4,803,319	-				+0.4
4,803,348	-				+0.2
4,803,390	+				-1.5
4,803,400	+				-0.4
4,803,400	+				+0.8
4,803,400	+				+0.5
4,803,400	+				+0.3
4,803,401	-				+0.1
4,803,401	-				+0.7
4,803,401	-				-0.6
4,803,401	-				-0.7
4,803,401	-				-0.2
4,803,401	-				+1.2
4,803,401	-				+0.7
4,803,404	+				-0.8
4,803,404	+				-0.5
4,803,405	-				-1.7
4,803,405	-				+1.4
4,803,614	+		PP_4232	0.25	+0.1
4,803,615	-		PP_4232	0.25	+0.8
4,803,615	-		PP_4232	0.25	-0.9
4,803,615	-		PP_4232	0.25	+0.3
4,803,618	+		PP_4232	0.25	-0.9
4,803,618	+		PP_4232	0.25	-1.3
4,803,619	-		PP_4232	0.25	+1.8
4,803,711	+		PP_4232	0.33	+0.7
4,803,711	+		PP_4232	0.33	-1.2
4,803,712	-		PP_4232	0.33	+0.0
4,803,891	+		PP_4232	0.47	+1.0
4,803,891	+		PP_4232	0.47	-0.4
4,803,891	+		PP_4232	0.47	+2.0
4,803,996	+		PP_4232	0.56	+0.7
4,803,996	+		PP_4232	0.56	-0.2
4,804,059	+		PP_4232	0.61	-0.4
4,804,060	-		PP_4232	0.61	+0.1
4,804,060	-		PP_4232	0.61	+0.5
4,804,061	+		PP_4232	0.61	+0.1
4,804,061	+		PP_4232	0.61	+0.9
4,804,061	+		PP_4232	0.61	-0.0
4,804,061	+		PP_4232	0.61	-1.2
4,804,061	+		PP_4232	0.61	-1.2
4,804,061	+		PP_4232	0.61	+0.4
4,804,061	+		PP_4232	0.61	-1.4
4,804,061	+		PP_4232	0.61	+0.8
4,804,061	+		PP_4232	0.61	-0.2
4,804,061	+		PP_4232	0.61	+1.0
4,804,061	+		PP_4232	0.61	+0.3
4,804,061	+		PP_4232	0.61	+0.1
4,804,061	+		PP_4232	0.61	+1.8
4,804,061	+		PP_4232	0.61	-0.5
4,804,061	+		PP_4232	0.61	-2.9
4,804,062	-				+1.5
4,804,062	-		PP_4232	0.61	-1.3
4,804,062	-		PP_4232	0.61	+0.5
4,804,062	-		PP_4232	0.61	-1.7
4,804,062	-		PP_4232	0.61	-2.0
4,804,062	-		PP_4232	0.61	+0.9
4,804,146	-		PP_4232	0.68	+0.1
4,804,200	+		PP_4232	0.73	+0.1
4,804,200	+		PP_4232	0.73	+0.5
4,804,200	+		PP_4232	0.73	-1.4
4,804,201	-		PP_4232	0.73	-0.6
4,804,210	-				+0.8
4,804,287	+		PP_4232	0.80	-0.0
4,804,287	+		PP_4232	0.80	+1.2
4,804,288	-		PP_4232	0.80	-0.2
4,804,321	+		PP_4232	0.83	-0.1
4,804,321	+		PP_4232	0.83	+0.9
4,804,321	+		PP_4232	0.83	+1.1
4,804,322	-		PP_4232	0.83	+0.2
4,804,322	-		PP_4232	0.83	+0.6
4,804,332	+		PP_4232	0.84	-0.4
4,804,332	+		PP_4232	0.84	-0.2
4,804,333	-		PP_4232	0.84	-1.7
4,804,333	-		PP_4232	0.84	+0.4
4,804,333	-		PP_4232	0.84	-2.2
4,804,333	-		PP_4232	0.84	-0.3
4,804,375	-		PP_4232	0.87	-0.5
4,804,431	+				+0.6
4,804,431	+				-2.1
4,804,514	-				+0.6
4,804,517	+				+2.1
4,804,517	+				+0.2
4,804,517	+				+1.3
4,804,583	+				-0.5
4,804,583	+				-0.1
4,804,583	+				+1.5
4,804,583	+				+1.6
4,804,584	-				-1.1
4,804,584	-				+0.2
4,804,584	-				-0.5
4,804,584	-				-0.5
4,804,586	+		PP_4233	0.10	+0.7
4,804,586	+		PP_4233	0.10	+0.1
4,804,595	+		PP_4233	0.12	+1.1
4,804,596	-		PP_4233	0.12	+0.1
4,804,596	-		PP_4233	0.12	-1.0
4,804,596	-		PP_4233	0.12	-0.1
4,804,727	+		PP_4233	0.36	+0.4
4,804,727	+		PP_4233	0.36	-0.5
4,804,848	-		PP_4233	0.59	-0.2
4,805,316	+				+0.0
4,805,316	+				+1.1
4,805,499	+		PP_4234	0.16	-0.5
4,805,499	+		PP_4234	0.16	+1.3
4,805,500	-		PP_4234	0.16	+0.9
4,805,500	-		PP_4234	0.16	+1.2
4,805,500	-		PP_4234	0.16	+0.7
4,805,500	-		PP_4234	0.16	-0.7
4,805,502	+		PP_4234	0.16	-1.1
4,805,502	+		PP_4234	0.16	-1.6
4,805,502	+		PP_4234	0.16	-1.0
4,805,502	+		PP_4234	0.16	-1.1
4,805,503	-		PP_4234	0.16	+0.1
4,805,503	-		PP_4234	0.16	+0.0
4,805,503	-		PP_4234	0.16	+0.3