Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4202 and PP_4203 are separated by 535 nucleotides PP_4203 and PP_4204 are separated by 71 nucleotides PP_4204 and PP_4205 are separated by 2 nucleotides PP_4205 and PP_4206 are separated by 94 nucleotides
PP_4202: PP_4202 - Electron transfer flavoprotein subunit beta, at 4,747,012 to 4,747,761
_4202
PP_4203: PP_4203 - Electron transfer flavoprotein-ubiquinone oxidoreductase, at 4,748,297 to 4,749,979
_4203
PP_4204: PP_4204 - antitoxin of the type II toxin-antitoxin MqsRA system and DNA-binding transcriptional repressor, at 4,750,051 to 4,750,452
_4204
PP_4205: PP_4205 - mRNA interferase MqsR of the MqsA-MqsR antitoxin/toxin complex and DNA-binding transcriptional repressor, at 4,750,455 to 4,750,751
_4205
PP_4206: PP_4206 - Major facilitator family transporter, at 4,750,846 to 4,752,171
_4206
Position (kb)
4748
4749
4750 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4747.788 kb on - strand at 4747.788 kb on - strand at 4747.828 kb on - strand at 4747.949 kb on - strand at 4747.995 kb on - strand at 4747.995 kb on - strand at 4748.003 kb on - strand at 4748.003 kb on - strand at 4748.025 kb on - strand at 4748.025 kb on - strand at 4748.048 kb on - strand at 4748.172 kb on + strand at 4748.172 kb on + strand at 4748.172 kb on + strand at 4748.176 kb on + strand at 4748.176 kb on + strand at 4748.176 kb on + strand at 4748.178 kb on + strand at 4748.178 kb on + strand at 4748.204 kb on + strand at 4748.224 kb on + strand at 4748.224 kb on + strand at 4748.242 kb on + strand at 4748.242 kb on + strand at 4748.242 kb on + strand at 4748.270 kb on + strand at 4749.977 kb on + strand at 4749.977 kb on + strand at 4749.978 kb on - strand at 4749.978 kb on - strand at 4749.978 kb on - strand at 4749.978 kb on - strand at 4750.060 kb on - strand at 4750.222 kb on + strand, within PP_4204 at 4750.222 kb on + strand, within PP_4204 at 4750.222 kb on + strand, within PP_4204 at 4750.233 kb on + strand, within PP_4204 at 4750.234 kb on - strand, within PP_4204 at 4750.234 kb on - strand, within PP_4204 at 4750.358 kb on - strand, within PP_4204 at 4750.389 kb on - strand, within PP_4204 at 4750.389 kb on - strand, within PP_4204 at 4750.389 kb on - strand, within PP_4204 at 4750.393 kb on - strand, within PP_4204 at 4750.393 kb on - strand, within PP_4204 at 4750.393 kb on - strand, within PP_4204 at 4750.395 kb on - strand, within PP_4204 at 4750.395 kb on - strand, within PP_4204 at 4750.395 kb on - strand, within PP_4204 at 4750.395 kb on - strand, within PP_4204 at 4750.438 kb on + strand at 4750.456 kb on + strand at 4750.457 kb on - strand at 4750.491 kb on + strand, within PP_4205 at 4750.510 kb on + strand, within PP_4205 at 4750.511 kb on - strand, within PP_4205 at 4750.511 kb on - strand, within PP_4205 at 4750.529 kb on + strand, within PP_4205 at 4750.529 kb on + strand, within PP_4205 at 4750.530 kb on - strand, within PP_4205 at 4750.541 kb on - strand, within PP_4205 at 4750.568 kb on + strand at 4750.588 kb on + strand, within PP_4205 at 4750.588 kb on + strand, within PP_4205 at 4750.589 kb on - strand, within PP_4205 at 4750.785 kb on + strand at 4750.785 kb on + strand at 4750.785 kb on + strand at 4750.785 kb on + strand at 4750.786 kb on - strand at 4750.786 kb on - strand at 4750.786 kb on - strand at 4750.786 kb on - strand at 4750.786 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,747,788 - +1.0 4,747,788 - -0.5 4,747,828 - -1.8 4,747,949 - -0.6 4,747,995 - -1.0 4,747,995 - +0.8 4,748,003 - -2.1 4,748,003 - +0.4 4,748,025 - -0.0 4,748,025 - +0.9 4,748,048 - +0.8 4,748,172 + +1.8 4,748,172 + -0.2 4,748,172 + +1.3 4,748,176 + +1.9 4,748,176 + -1.1 4,748,176 + +0.3 4,748,178 + +0.8 4,748,178 + -0.6 4,748,204 + +0.3 4,748,224 + +1.3 4,748,224 + +0.7 4,748,242 + -1.7 4,748,242 + -1.6 4,748,242 + -1.0 4,748,270 + -0.1 4,749,977 + +0.6 4,749,977 + +0.8 4,749,978 - +0.2 4,749,978 - +0.1 4,749,978 - -0.7 4,749,978 - -1.5 4,750,060 - -1.3 4,750,222 + PP_4204 0.43 +0.2 4,750,222 + PP_4204 0.43 +0.3 4,750,222 + PP_4204 0.43 -1.6 4,750,233 + PP_4204 0.45 -0.3 4,750,234 - PP_4204 0.46 +0.7 4,750,234 - PP_4204 0.46 -0.7 4,750,358 - PP_4204 0.76 -0.1 4,750,389 - PP_4204 0.84 -0.9 4,750,389 - PP_4204 0.84 +0.8 4,750,389 - PP_4204 0.84 +2.5 4,750,393 - PP_4204 0.85 +1.0 4,750,393 - PP_4204 0.85 -1.3 4,750,393 - PP_4204 0.85 -0.9 4,750,395 - PP_4204 0.86 +0.9 4,750,395 - PP_4204 0.86 -0.5 4,750,395 - PP_4204 0.86 -0.5 4,750,395 - PP_4204 0.86 -0.5 4,750,438 + +1.0 4,750,456 + +1.3 4,750,457 - -1.6 4,750,491 + PP_4205 0.12 -1.8 4,750,510 + PP_4205 0.19 +0.2 4,750,511 - PP_4205 0.19 -0.5 4,750,511 - PP_4205 0.19 -1.4 4,750,529 + PP_4205 0.25 -0.8 4,750,529 + PP_4205 0.25 -1.8 4,750,530 - PP_4205 0.25 -0.8 4,750,541 - PP_4205 0.29 -0.1 4,750,568 + +0.2 4,750,588 + PP_4205 0.45 +0.1 4,750,588 + PP_4205 0.45 -2.0 4,750,589 - PP_4205 0.45 +0.5 4,750,785 + -1.7 4,750,785 + -0.4 4,750,785 + -0.9 4,750,785 + -0.4 4,750,786 - -0.2 4,750,786 - -0.4 4,750,786 - -1.5 4,750,786 - +0.8 4,750,786 - +0.1
Or see this region's nucleotide sequence