Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4161 and PP_5660 are separated by 9 nucleotides PP_5660 and PP_4162 are separated by 176 nucleotides PP_4162 and PP_4163 are separated by 12 nucleotides PP_4163 and PP_4164 are separated by 109 nucleotides
PP_4161: PP_4161 - potassium-transporting ATPase A chain, at 4,703,469 to 4,705,163
_4161
PP_5660: PP_5660 - potassium-transporting ATPase F, at 4,705,173 to 4,705,262
_5660
PP_4162: PP_4162 - conserved membrane protein of unknown function, at 4,705,439 to 4,706,491
_4162
PP_4163: PP_4163 - conserved exported protein of unknown function, at 4,706,504 to 4,707,298
_4163
PP_4164: PP_4164 - Alpha/beta hydrolase, at 4,707,408 to 4,708,262
_4164
Position (kb)
4705
4706
4707 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4704.465 kb on - strand, within PP_4161 at 4704.775 kb on + strand, within PP_4161 at 4704.775 kb on + strand, within PP_4161 at 4704.775 kb on + strand, within PP_4161 at 4704.776 kb on - strand, within PP_4161 at 4704.799 kb on + strand, within PP_4161 at 4704.967 kb on + strand, within PP_4161 at 4704.967 kb on + strand, within PP_4161 at 4704.967 kb on + strand, within PP_4161 at 4704.968 kb on - strand, within PP_4161 at 4704.968 kb on - strand, within PP_4161 at 4705.081 kb on + strand at 4705.082 kb on - strand at 4705.082 kb on - strand at 4705.082 kb on - strand at 4705.082 kb on - strand at 4705.084 kb on + strand at 4705.153 kb on + strand at 4705.154 kb on - strand at 4705.174 kb on + strand at 4705.174 kb on + strand at 4705.174 kb on + strand at 4705.174 kb on + strand at 4705.324 kb on + strand at 4705.324 kb on + strand at 4705.325 kb on - strand at 4705.357 kb on + strand at 4705.368 kb on + strand at 4705.433 kb on - strand at 4705.433 kb on - strand at 4705.440 kb on + strand at 4705.440 kb on + strand at 4705.440 kb on + strand at 4705.441 kb on - strand at 4705.441 kb on - strand at 4705.906 kb on - strand, within PP_4162 at 4706.077 kb on - strand, within PP_4162 at 4706.092 kb on + strand, within PP_4162 at 4706.092 kb on + strand, within PP_4162 at 4706.092 kb on + strand, within PP_4162 at 4706.092 kb on + strand, within PP_4162 at 4706.093 kb on - strand, within PP_4162 at 4706.225 kb on - strand, within PP_4162 at 4706.672 kb on + strand, within PP_4163 at 4706.706 kb on + strand, within PP_4163 at 4706.747 kb on - strand, within PP_4163 at 4706.809 kb on - strand, within PP_4163 at 4706.809 kb on - strand, within PP_4163 at 4706.839 kb on - strand, within PP_4163 at 4706.839 kb on - strand, within PP_4163 at 4707.009 kb on - strand, within PP_4163 at 4707.050 kb on - strand, within PP_4163 at 4707.069 kb on + strand, within PP_4163 at 4707.434 kb on + strand at 4707.434 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,704,465 - PP_4161 0.59 +0.4 4,704,775 + PP_4161 0.77 -1.0 4,704,775 + PP_4161 0.77 +0.3 4,704,775 + PP_4161 0.77 +0.1 4,704,776 - PP_4161 0.77 -0.4 4,704,799 + PP_4161 0.78 +0.9 4,704,967 + PP_4161 0.88 -0.0 4,704,967 + PP_4161 0.88 +0.7 4,704,967 + PP_4161 0.88 -0.7 4,704,968 - PP_4161 0.88 -1.2 4,704,968 - PP_4161 0.88 +1.7 4,705,081 + +0.3 4,705,082 - -0.2 4,705,082 - -1.0 4,705,082 - +1.1 4,705,082 - +0.7 4,705,084 + +0.1 4,705,153 + -0.8 4,705,154 - -0.7 4,705,174 + -1.2 4,705,174 + +0.5 4,705,174 + +0.4 4,705,174 + -0.5 4,705,324 + -0.5 4,705,324 + +0.5 4,705,325 - -1.6 4,705,357 + -0.8 4,705,368 + -2.3 4,705,433 - -1.5 4,705,433 - +0.4 4,705,440 + +1.7 4,705,440 + -0.6 4,705,440 + -0.7 4,705,441 - -0.4 4,705,441 - -2.3 4,705,906 - PP_4162 0.44 +1.8 4,706,077 - PP_4162 0.61 +0.6 4,706,092 + PP_4162 0.62 +1.0 4,706,092 + PP_4162 0.62 +1.0 4,706,092 + PP_4162 0.62 -0.9 4,706,092 + PP_4162 0.62 +1.3 4,706,093 - PP_4162 0.62 -0.9 4,706,225 - PP_4162 0.75 -1.5 4,706,672 + PP_4163 0.21 +1.9 4,706,706 + PP_4163 0.25 -0.1 4,706,747 - PP_4163 0.31 +1.0 4,706,809 - PP_4163 0.38 -1.4 4,706,809 - PP_4163 0.38 -0.6 4,706,839 - PP_4163 0.42 +0.7 4,706,839 - PP_4163 0.42 +0.5 4,707,009 - PP_4163 0.64 -0.3 4,707,050 - PP_4163 0.69 -1.0 4,707,069 + PP_4163 0.71 -0.6 4,707,434 + +0.4 4,707,434 + -0.7
Or see this region's nucleotide sequence