Strain Fitness in Pseudomonas putida KT2440 around PP_4049

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,560,566	+		PP_4048	0.30	+1.6
4,560,566	+		PP_4048	0.30	-1.6
4,560,566	+		PP_4048	0.30	-0.5
4,560,567	-		PP_4048	0.30	-0.6
4,560,567	-		PP_4048	0.30	+0.2
4,560,571	-		PP_4048	0.30	-0.1
4,560,884	+		PP_4048	0.53	-0.4
4,560,885	-		PP_4048	0.53	+0.2
4,560,960	-		PP_4048	0.58	+1.0
4,561,014	-		PP_4048	0.62	-1.9
4,561,034	+		PP_4048	0.63	-2.3
4,561,034	+		PP_4048	0.63	+1.4
4,561,136	-		PP_4048	0.71	-0.3
4,561,136	-		PP_4048	0.71	+0.7
4,561,136	-		PP_4048	0.71	-0.2
4,561,696	+				+0.7
4,561,696	+				+0.4
4,561,697	-				-3.0
4,561,753	+				+0.3
4,561,753	+				+1.5
4,561,776	-				+0.8
4,561,830	+		PP_4049	0.12	-1.3
4,561,837	+		PP_4049	0.13	-1.5
4,561,837	+		PP_4049	0.13	+1.1
4,561,873	-		PP_4049	0.14	-1.9
4,561,970	+		PP_4049	0.18	-1.6
4,561,970	+		PP_4049	0.18	+0.9
4,562,097	+		PP_4049	0.24	+1.1
4,562,097	+		PP_4049	0.24	-0.7
4,562,098	-		PP_4049	0.24	+2.4
4,562,307	+		PP_4049	0.33	+2.4
4,562,307	+		PP_4049	0.33	+0.3
4,562,307	+		PP_4049	0.33	-2.0
4,562,307	+		PP_4049	0.33	+1.0
4,562,307	+		PP_4049	0.33	+0.1
4,562,307	+		PP_4049	0.33	+0.6
4,562,307	+		PP_4049	0.33	-0.9
4,562,308	-		PP_4049	0.33	+0.3
4,562,308	-		PP_4049	0.33	+1.5
4,562,308	-		PP_4049	0.33	-1.4
4,562,419	+		PP_4049	0.38	-0.5
4,562,489	-		PP_4049	0.41	-0.2
4,562,489	-		PP_4049	0.41	-0.7
4,562,489	-		PP_4049	0.41	-0.1
4,562,650	+		PP_4049	0.48	-1.3
4,562,650	+		PP_4049	0.48	-1.0
4,562,650	+		PP_4049	0.48	-0.4
4,562,650	+		PP_4049	0.48	-0.0
4,562,650	+		PP_4049	0.48	+0.5
4,562,651	-		PP_4049	0.48	+3.4
4,562,651	-		PP_4049	0.48	-0.3
4,562,651	-		PP_4049	0.48	-0.8
4,562,770	+		PP_4049	0.53	-0.5
4,562,770	+		PP_4049	0.53	-1.3
4,562,826	-		PP_4049	0.55	+0.7
4,562,826	-		PP_4049	0.55	-0.3
4,563,013	+		PP_4049	0.63	+0.4
4,563,041	-		PP_4049	0.65	-0.2
4,563,041	-		PP_4049	0.65	+0.9
4,563,041	-		PP_4049	0.65	+0.4
4,563,116	-		PP_4049	0.68	+0.8
4,563,116	-				+1.3
4,563,140	+		PP_4049	0.69	-0.4
4,563,140	+		PP_4049	0.69	-1.5
4,563,140	+		PP_4049	0.69	+0.9
4,563,140	+		PP_4049	0.69	+1.0
4,563,141	-		PP_4049	0.69	-0.3
4,563,141	-		PP_4049	0.69	+1.8
4,563,141	-		PP_4049	0.69	+0.5
4,563,144	+		PP_4049	0.69	+0.5
4,563,145	-		PP_4049	0.69	+1.4
4,563,145	-		PP_4049	0.69	-1.9
4,563,157	+		PP_4049	0.70	+0.4
4,563,158	-		PP_4049	0.70	-0.2
4,563,211	+		PP_4049	0.72	+0.9
4,563,211	+		PP_4049	0.72	-0.5
4,563,211	+		PP_4049	0.72	-1.5
4,563,676	+				-0.8
4,563,687	+				+0.8
4,563,687	+				-1.7
4,563,931	+				-0.4
4,563,972	-				-1.1
4,564,079	+				-0.8
4,564,139	+				+0.5
4,564,140	-				-0.3
4,564,214	-				+0.2
4,564,243	+				-2.0
4,564,245	+				-1.6
4,564,245	+				-1.7
4,564,246	-				+0.3
4,564,246	-				-1.4
4,564,298	+				-1.8
4,564,299	-				+0.1
4,564,299	-				+0.1
4,564,369	+				-0.6
4,564,369	+				-1.1
4,564,369	+				+1.0
4,564,370	-				-2.0
4,564,390	-				-0.9
4,564,390	-				-0.7
4,564,407	+				+1.7
4,564,463	+				+0.1
4,564,549	+				+0.3
4,564,549	+				-0.7
4,564,549	+				-0.8
4,564,549	+				+0.2
4,564,549	+				+1.7
4,564,550	-				-0.3
4,564,550	-				+1.9
4,564,550	-				+0.1
4,564,550	-				+0.3
4,564,550	-				-2.4
4,564,553	+				-0.5
4,564,553	+				-0.5
4,564,741	+				+0.7
4,564,741	+				-0.2
4,564,742	-				+1.4
4,564,742	-				-2.3
4,564,742	-				-0.6
4,564,757	+				-0.3
4,564,757	+				+0.8
4,564,757	+				+1.1
4,564,758	-				+0.2
4,564,758	-				+0.9
4,564,758	-				+0.1