Strain Fitness in Pseudomonas putida KT2440 around PP_4047

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,558,307	+		PP_4046	0.11	-2.1
4,558,307	+		PP_4046	0.11	-0.9
4,558,307	+		PP_4046	0.11	-0.3
4,558,308	-		PP_4046	0.11	+0.1
4,558,308	-		PP_4046	0.11	+0.4
4,558,480	+		PP_4046	0.29	+0.8
4,558,480	+		PP_4046	0.29	-0.9
4,558,480	+		PP_4046	0.29	-1.2
4,558,489	+		PP_4046	0.30	-0.9
4,558,559	+		PP_4046	0.37	-0.3
4,558,580	+		PP_4046	0.39	+0.9
4,558,580	+		PP_4046	0.39	-2.4
4,558,580	+		PP_4046	0.39	+0.9
4,558,581	-		PP_4046	0.39	+0.4
4,558,581	-		PP_4046	0.39	-0.5
4,558,596	-		PP_4046	0.41	-1.2
4,558,658	+		PP_4046	0.47	-1.2
4,558,658	+		PP_4046	0.47	+0.6
4,558,659	-		PP_4046	0.48	-0.7
4,558,691	+		PP_4046	0.51	-2.3
4,558,692	-		PP_4046	0.51	+1.3
4,558,919	+		PP_4046	0.75	+1.6
4,558,928	+		PP_4046	0.75	+0.6
4,558,928	+		PP_4046	0.75	+1.0
4,558,929	-		PP_4046	0.76	-0.9
4,558,929	-		PP_4046	0.76	+0.7
4,558,965	-		PP_4046	0.79	+0.4
4,559,010	+		PP_4046	0.84	-2.5
4,559,011	-		PP_4046	0.84	-1.1
4,559,064	+		PP_4046	0.90	-1.1
4,559,064	+		PP_4046	0.90	-1.0
4,559,065	-		PP_4046	0.90	-0.1
4,559,111	+				-0.6
4,559,112	-				-1.1
4,559,163	+				-0.4
4,559,164	-				-2.2
4,559,180	+				-1.9
4,559,181	-				-0.7
4,559,181	-				+0.6
4,559,181	-				-0.9
4,559,181	-				-0.9
4,559,182	+				+1.7
4,559,182	+				-1.9
4,559,183	-				+0.3
4,559,183	-				-1.6
4,559,183	-				-0.4
4,559,236	+				-1.0
4,559,263	+				-2.5
4,559,264	-				-0.8
4,559,281	+		PP_4047	0.11	+0.4
4,559,281	+		PP_4047	0.11	-0.7
4,559,282	-		PP_4047	0.11	-2.2
4,559,337	-		PP_4047	0.17	-0.5
4,559,415	-		PP_4047	0.25	-0.3
4,559,432	+		PP_4047	0.26	-0.8
4,559,433	-				+1.1
4,559,537	+		PP_4047	0.37	-0.4
4,559,577	+		PP_4047	0.42	-1.9
4,559,577	+		PP_4047	0.42	+1.4
4,559,577	+		PP_4047	0.42	+2.1
4,559,578	-		PP_4047	0.42	+0.8
4,559,794	+		PP_4047	0.64	-1.3
4,559,795	-		PP_4047	0.64	-1.5
4,559,896	+		PP_4047	0.75	+0.4
4,559,925	+				+2.2
4,559,926	-		PP_4047	0.78	+0.6
4,559,926	-		PP_4047	0.78	-1.6
4,559,998	+		PP_4047	0.86	-1.1
4,559,999	-				+1.3
4,560,155	-				+0.9
4,560,242	+				-1.8
4,560,287	-		PP_4048	0.10	-0.5
4,560,297	+		PP_4048	0.11	-0.1
4,560,298	-		PP_4048	0.11	+0.1
4,560,345	+		PP_4048	0.14	-0.2
4,560,415	-		PP_4048	0.19	-1.5
4,560,441	+		PP_4048	0.21	+0.1
4,560,441	+		PP_4048	0.21	+0.1
4,560,446	+		PP_4048	0.21	+0.2
4,560,447	-		PP_4048	0.22	+0.3
4,560,542	+		PP_4048	0.28	-1.2
4,560,542	+		PP_4048	0.28	-1.8
4,560,542	+		PP_4048	0.28	+1.2
4,560,542	+		PP_4048	0.28	-1.4
4,560,543	-		PP_4048	0.28	-0.1
4,560,543	-		PP_4048	0.28	+0.4
4,560,543	-		PP_4048	0.28	+0.2
4,560,543	-		PP_4048	0.28	-1.7
4,560,543	-		PP_4048	0.28	-1.7
4,560,543	-		PP_4048	0.28	+0.4
4,560,543	-		PP_4048	0.28	+1.1
4,560,566	+		PP_4048	0.30	+1.6
4,560,566	+		PP_4048	0.30	-1.6
4,560,566	+		PP_4048	0.30	-0.5
4,560,567	-		PP_4048	0.30	-0.6
4,560,567	-		PP_4048	0.30	+0.2
4,560,571	-		PP_4048	0.30	-0.1
4,560,884	+		PP_4048	0.53	-0.4
4,560,885	-		PP_4048	0.53	+0.2
4,560,960	-		PP_4048	0.58	+1.0
4,561,014	-		PP_4048	0.62	-1.9
4,561,034	+		PP_4048	0.63	-2.3
4,561,034	+		PP_4048	0.63	+1.4