Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4000 and PP_4001 are separated by 30 nucleotides PP_4001 and PP_4002 overlap by 4 nucleotides PP_4002 and PP_4003 are separated by 12 nucleotides PP_4003 and PP_4004 overlap by 1 nucleotides
PP_4000: PP_4000 - Serine--tRNA ligase, at 4,508,781 to 4,510,061
_4000
PP_4001: PP_4001 - Putative fluoride ion transporter CrcB, at 4,510,092 to 4,510,466
_4001
PP_4002: PP_4002 - DNA-dependent ATPase required for recombination, at 4,510,463 to 4,511,788
_4002
PP_4003: PP_4003 - Outer-membrane lipoprotein carrier protein, at 4,511,801 to 4,512,487
_4003
PP_4004: PP_4004 - DNA translocase FtsK, at 4,512,487 to 4,514,991
_4004
Position (kb)
4510
4511
4512 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.076 kb on - strand at 4510.078 kb on - strand at 4510.078 kb on - strand at 4510.078 kb on - strand at 4510.078 kb on - strand at 4510.082 kb on - strand at 4510.082 kb on - strand at 4510.082 kb on - strand at 4510.082 kb on - strand at 4510.082 kb on - strand at 4510.152 kb on - strand, within PP_4001 at 4510.158 kb on - strand, within PP_4001 at 4510.158 kb on - strand, within PP_4001 at 4510.158 kb on - strand, within PP_4001 at 4510.160 kb on - strand, within PP_4001 at 4510.364 kb on - strand, within PP_4001 at 4510.364 kb on - strand, within PP_4001 at 4510.404 kb on - strand, within PP_4001 at 4510.422 kb on - strand, within PP_4001 at 4510.422 kb on - strand, within PP_4001 at 4510.422 kb on - strand, within PP_4001 at 4510.422 kb on - strand, within PP_4001 at 4510.563 kb on + strand at 4510.564 kb on - strand at 4510.564 kb on - strand at 4510.564 kb on - strand at 4510.566 kb on + strand at 4510.567 kb on - strand at 4510.567 kb on - strand at 4510.597 kb on - strand, within PP_4002 at 4510.597 kb on - strand, within PP_4002 at 4510.597 kb on - strand, within PP_4002 at 4510.597 kb on - strand, within PP_4002 at 4510.615 kb on - strand, within PP_4002 at 4510.639 kb on - strand, within PP_4002 at 4510.639 kb on - strand, within PP_4002 at 4510.761 kb on + strand, within PP_4002 at 4510.761 kb on + strand, within PP_4002 at 4510.762 kb on - strand, within PP_4002 at 4510.762 kb on - strand, within PP_4002 at 4510.794 kb on + strand, within PP_4002 at 4510.876 kb on + strand, within PP_4002 at 4510.941 kb on + strand, within PP_4002 at 4511.033 kb on + strand, within PP_4002 at 4511.033 kb on + strand, within PP_4002 at 4511.099 kb on + strand, within PP_4002 at 4511.099 kb on + strand, within PP_4002 at 4511.099 kb on + strand, within PP_4002 at 4511.099 kb on + strand, within PP_4002 at 4511.100 kb on - strand, within PP_4002 at 4511.100 kb on - strand, within PP_4002 at 4511.316 kb on + strand, within PP_4002 at 4511.316 kb on + strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.317 kb on - strand, within PP_4002 at 4511.410 kb on - strand, within PP_4002 at 4511.484 kb on + strand, within PP_4002 at 4511.484 kb on + strand, within PP_4002 at 4511.715 kb on + strand at 4511.716 kb on - strand at 4511.716 kb on - strand at 4511.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,510,076 - -1.8 4,510,076 - +0.3 4,510,076 - -0.9 4,510,076 - +0.7 4,510,076 - -1.3 4,510,076 - +0.4 4,510,076 - -0.3 4,510,078 - -0.8 4,510,078 - +0.0 4,510,078 - -0.3 4,510,078 - +0.6 4,510,082 - +0.5 4,510,082 - -0.4 4,510,082 - -1.4 4,510,082 - -0.2 4,510,082 - +0.5 4,510,152 - PP_4001 0.16 +0.2 4,510,158 - PP_4001 0.18 +0.2 4,510,158 - PP_4001 0.18 +0.6 4,510,158 - PP_4001 0.18 -1.5 4,510,160 - PP_4001 0.18 -0.5 4,510,364 - PP_4001 0.73 -0.4 4,510,364 - PP_4001 0.73 +0.2 4,510,404 - PP_4001 0.83 +0.2 4,510,422 - PP_4001 0.88 -0.3 4,510,422 - PP_4001 0.88 -0.5 4,510,422 - PP_4001 0.88 -1.3 4,510,422 - PP_4001 0.88 -1.1 4,510,563 + +1.9 4,510,564 - -0.0 4,510,564 - -1.8 4,510,564 - +1.3 4,510,566 + +1.9 4,510,567 - +0.8 4,510,567 - +1.3 4,510,597 - PP_4002 0.10 +0.1 4,510,597 - PP_4002 0.10 +1.6 4,510,597 - PP_4002 0.10 +0.3 4,510,597 - PP_4002 0.10 -1.2 4,510,615 - PP_4002 0.11 -1.0 4,510,639 - PP_4002 0.13 -0.7 4,510,639 - PP_4002 0.13 -1.4 4,510,761 + PP_4002 0.22 +2.9 4,510,761 + PP_4002 0.22 +0.8 4,510,762 - PP_4002 0.23 +0.1 4,510,762 - PP_4002 0.23 +1.2 4,510,794 + PP_4002 0.25 -0.5 4,510,876 + PP_4002 0.31 -1.0 4,510,941 + PP_4002 0.36 +0.2 4,511,033 + PP_4002 0.43 +0.9 4,511,033 + PP_4002 0.43 +0.1 4,511,099 + PP_4002 0.48 +0.6 4,511,099 + PP_4002 0.48 -0.4 4,511,099 + PP_4002 0.48 -0.1 4,511,099 + PP_4002 0.48 -0.8 4,511,100 - PP_4002 0.48 -1.8 4,511,100 - PP_4002 0.48 +0.7 4,511,316 + PP_4002 0.64 -1.7 4,511,316 + PP_4002 0.64 -0.5 4,511,317 - PP_4002 0.64 -0.9 4,511,317 - PP_4002 0.64 -1.2 4,511,317 - PP_4002 0.64 +0.3 4,511,317 - PP_4002 0.64 +0.2 4,511,317 - PP_4002 0.64 -0.1 4,511,317 - PP_4002 0.64 -1.3 4,511,317 - PP_4002 0.64 -1.2 4,511,410 - PP_4002 0.71 +0.3 4,511,484 + PP_4002 0.77 +1.0 4,511,484 + PP_4002 0.77 -1.4 4,511,715 + +0.6 4,511,716 - +1.4 4,511,716 - -0.8 4,511,803 - -0.2
Or see this region's nucleotide sequence