Strain Fitness in Pseudomonas putida KT2440 around PP_3976

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,482,680	-		PP_3974	0.69	-2.0
4,482,708	+		PP_3974	0.72	+0.8
4,482,708	+		PP_3974	0.72	+1.5
4,482,750	+		PP_3974	0.76	+0.4
4,482,750	+		PP_3974	0.76	+1.6
4,482,751	-		PP_3974	0.77	-1.6
4,482,896	+				-0.6
4,482,897	-				+1.1
4,482,979	+				+0.7
4,483,075	+		PP_3975	0.16	-0.3
4,483,085	-		PP_3975	0.18	+0.4
4,483,085	-		PP_3975	0.18	-1.7
4,483,085	-		PP_3975	0.18	-0.2
4,483,085	-		PP_3975	0.18	-0.9
4,483,087	+		PP_3975	0.19	+0.2
4,483,087	+		PP_3975	0.19	-0.4
4,483,088	-		PP_3975	0.19	+0.9
4,483,088	-		PP_3975	0.19	+0.5
4,483,116	+		PP_3975	0.25	+0.8
4,483,117	-		PP_3975	0.25	-1.0
4,483,135	+		PP_3975	0.29	+0.5
4,483,173	+		PP_3975	0.37	+0.9
4,483,173	+		PP_3975	0.37	+0.5
4,483,173	+		PP_3975	0.37	+0.6
4,483,174	-		PP_3975	0.37	-0.0
4,483,174	-		PP_3975	0.37	-0.2
4,483,174	-		PP_3975	0.37	+1.5
4,483,174	-		PP_3975	0.37	+1.1
4,483,174	-		PP_3975	0.37	-0.2
4,483,212	+		PP_3975	0.46	-1.6
4,483,212	+		PP_3975	0.46	-1.9
4,483,212	+		PP_3975	0.46	-1.2
4,483,213	-		PP_3975	0.46	-2.2
4,483,213	-		PP_3975	0.46	+1.0
4,483,213	-		PP_3975	0.46	-0.1
4,483,213	-		PP_3975	0.46	-0.7
4,483,213	-		PP_3975	0.46	+0.2
4,483,213	-		PP_3975	0.46	+0.7
4,483,213	-		PP_3975	0.46	+1.2
4,483,243	-		PP_3975	0.52	+1.1
4,483,425	+				+0.8
4,483,426	-				-2.1
4,483,515	+				-1.4
4,483,515	+				-0.2
4,483,516	-				-1.7
4,483,516	-				-1.2
4,483,516	-				+1.4
4,483,600	+				-1.6
4,483,600	+				+0.0
4,483,601	-				+1.2
4,483,795	-		PP_3976	0.18	+1.6
4,483,873	+		PP_3976	0.30	-0.2
4,483,873	+		PP_3976	0.30	-0.3
4,483,873	+		PP_3976	0.30	-0.8
4,483,874	-		PP_3976	0.30	-0.6
4,483,874	-		PP_3976	0.30	-1.0
4,483,874	-		PP_3976	0.30	-0.3
4,483,878	-		PP_3976	0.30	-0.4
4,484,063	+		PP_3976	0.57	+0.4
4,484,063	+		PP_3976	0.57	+0.3
4,484,064	-		PP_3976	0.57	+2.4
4,484,064	-		PP_3976	0.57	-1.6
4,484,072	+		PP_3976	0.59	+0.4
4,484,072	+		PP_3976	0.59	+1.0
4,484,072	+		PP_3976	0.59	-1.1
4,484,072	+		PP_3976	0.59	-0.1
4,484,072	+		PP_3976	0.59	+0.5
4,484,072	+		PP_3976	0.59	+0.6
4,484,072	+		PP_3976	0.59	-2.5
4,484,072	+		PP_3976	0.59	+2.7
4,484,072	+		PP_3976	0.59	+1.1
4,484,072	+		PP_3976	0.59	+0.1
4,484,072	+		PP_3976	0.59	-0.8
4,484,072	+		PP_3976	0.59	-0.4
4,484,072	+		PP_3976	0.59	+0.1
4,484,072	+		PP_3976	0.59	+0.1
4,484,072	+		PP_3976	0.59	-0.4
4,484,073	-		PP_3976	0.59	+1.1
4,484,073	-		PP_3976	0.59	-0.2
4,484,073	-		PP_3976	0.59	+0.5
4,484,073	-		PP_3976	0.59	+0.5
4,484,073	-		PP_3976	0.59	+0.2
4,484,073	-		PP_3976	0.59	+1.2
4,484,073	-		PP_3976	0.59	-0.2
4,484,073	-		PP_3976	0.59	+0.5
4,484,073	-		PP_3976	0.59	+1.0
4,484,074	+		PP_3976	0.59	-1.5
4,484,074	+		PP_3976	0.59	-0.9
4,484,074	+		PP_3976	0.59	-1.3
4,484,075	-		PP_3976	0.59	-1.4
4,484,120	+		PP_3976	0.66	+0.5
4,484,121	-		PP_3976	0.66	-1.3
4,484,162	-		PP_3976	0.72	+2.5
4,484,178	+		PP_3976	0.74	-0.2
4,484,178	+		PP_3976	0.74	+1.3
4,484,179	-				+1.9
4,484,216	+		PP_3976	0.79	-2.3
4,484,216	+		PP_3976	0.79	-0.4
4,484,216	+		PP_3976	0.79	+1.0
4,484,216	+		PP_3976	0.79	+0.3
4,484,217	-		PP_3976	0.80	+1.0
4,484,217	-		PP_3976	0.80	+0.3
4,484,217	-		PP_3976	0.80	+0.9
4,484,276	+		PP_3976	0.88	-0.3
4,484,277	-		PP_3976	0.88	-2.0
4,484,282	+		PP_3976	0.89	-0.8
4,484,282	+		PP_3976	0.89	+1.3
4,484,282	+		PP_3976	0.89	+0.0
4,484,282	+		PP_3976	0.89	+2.2
4,484,282	+		PP_3976	0.89	+0.5
4,484,282	+		PP_3976	0.89	+0.5
4,484,283	-		PP_3976	0.89	+0.3
4,484,283	-		PP_3976	0.89	-0.6
4,484,283	-		PP_3976	0.89	-1.5
4,484,284	+		PP_3976	0.89	-0.9
4,484,284	+		PP_3976	0.89	+0.6
4,484,284	+		PP_3976	0.89	-0.5
4,484,284	+		PP_3976	0.89	+1.3
4,484,284	+		PP_3976	0.89	+1.0
4,484,285	-		PP_3976	0.90	-1.4
4,484,285	-		PP_3976	0.90	+0.3
4,484,487	+				+0.5
4,484,488	-				+1.0
4,484,488	-				-0.6
4,484,488	-				+0.3
4,484,545	+				+0.4
4,484,545	+				-0.2
4,484,545	+				-2.8
4,484,546	-				-1.0
4,484,548	-				+1.8
4,484,627	+				-0.6
4,484,676	+				-0.2
4,484,726	-				+0.6
4,484,784	-				+0.2
4,484,787	+				-0.9
4,484,788	-				-1.2
4,484,833	+				+0.7
4,484,834	-				-2.4
4,484,834	-				+1.5
4,484,834	-				-0.4
4,484,898	+				-0.4
4,484,898	+				-0.1
4,484,898	+				+0.2
4,484,899	-				+2.0
4,484,899	-				+0.4
4,484,928	+				+0.8
4,484,928	+				-0.3
4,485,108	+		PP_3977	0.22	-0.2
4,485,109	-		PP_3977	0.22	+1.1
4,485,157	+		PP_3977	0.25	-0.2
4,485,157	+		PP_3977	0.25	-0.9
4,485,158	-				+0.8
4,485,189	+		PP_3977	0.28	+0.1
4,485,189	+		PP_3977	0.28	+0.1
4,485,190	-		PP_3977	0.28	+1.1
4,485,190	-		PP_3977	0.28	-0.4
4,485,190	-		PP_3977	0.28	+0.1
4,485,199	+		PP_3977	0.29	+0.6
4,485,199	+		PP_3977	0.29	-0.3
4,485,200	-		PP_3977	0.29	-0.4
4,485,207	+		PP_3977	0.29	-0.3
4,485,208	-		PP_3977	0.29	-1.2
4,485,215	+		PP_3977	0.30	-0.7
4,485,216	-		PP_3977	0.30	+0.3
4,485,216	-		PP_3977	0.30	+0.5
4,485,216	-		PP_3977	0.30	+1.0
4,485,216	-		PP_3977	0.30	+1.0
4,485,237	+		PP_3977	0.32	+1.2
4,485,238	-		PP_3977	0.32	+0.3
4,485,277	-		PP_3977	0.35	-2.0
4,485,277	-		PP_3977	0.35	+0.0
4,485,277	-		PP_3977	0.35	+1.1
4,485,310	-		PP_3977	0.37	-0.0
4,485,318	+		PP_3977	0.38	+0.3
4,485,318	+		PP_3977	0.38	-2.0
4,485,318	+		PP_3977	0.38	-0.6