Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3945 and PP_3946 are separated by 70 nucleotides PP_3946 and PP_3947 are separated by 441 nucleotides PP_3947 and PP_3948 overlap by 4 nucleotides
PP_3945: PP_3945 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 4,447,886 to 4,448,938
_3945
PP_3946: PP_3946 - HTH-type transcriptional repressor NicR, at 4,449,009 to 4,449,485
_3946
PP_3947: PP_3947 - nicotinate dehydrogenase subunit A, at 4,449,927 to 4,450,400
_3947
PP_3948: PP_3948 - nicotinate dehydrogenase subunit B (EC 1.17.2.1) (from data) , at 4,450,397 to 4,453,960
_3948
Position (kb)
4449
4450
4451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4448.943 kb on + strand at 4448.944 kb on - strand at 4448.944 kb on - strand at 4448.945 kb on + strand at 4448.945 kb on + strand at 4448.945 kb on + strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4448.946 kb on - strand at 4449.008 kb on + strand at 4449.008 kb on + strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.009 kb on - strand at 4449.150 kb on - strand, within PP_3946 at 4449.150 kb on - strand, within PP_3946 at 4449.441 kb on + strand at 4449.680 kb on - strand at 4449.680 kb on - strand at 4449.689 kb on + strand at 4449.690 kb on - strand at 4449.701 kb on + strand at 4449.800 kb on + strand at 4449.800 kb on + strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.801 kb on - strand at 4449.851 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.858 kb on + strand at 4449.859 kb on - strand at 4449.859 kb on - strand at 4449.925 kb on + strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4449.926 kb on - strand at 4450.073 kb on + strand, within PP_3947 at 4450.073 kb on + strand, within PP_3947 at 4450.074 kb on - strand, within PP_3947 at 4450.074 kb on - strand, within PP_3947 at 4450.142 kb on + strand, within PP_3947 at 4450.142 kb on + strand, within PP_3947 at 4450.143 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.231 kb on - strand, within PP_3947 at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.513 kb on + strand at 4450.514 kb on - strand at 4450.571 kb on + strand at 4450.572 kb on - strand at 4450.613 kb on - strand at 4450.613 kb on - strand at 4450.782 kb on - strand, within PP_3948 at 4450.782 kb on - strand, within PP_3948 at 4450.782 kb on - strand, within PP_3948 at 4450.956 kb on - strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4450.986 kb on + strand, within PP_3948 at 4451.021 kb on + strand, within PP_3948 at 4451.021 kb on + strand, within PP_3948 at 4451.022 kb on - strand, within PP_3948 at 4451.022 kb on - strand, within PP_3948 at 4451.079 kb on - strand, within PP_3948 at 4451.284 kb on + strand, within PP_3948 at 4451.285 kb on - strand, within PP_3948 at 4451.396 kb on + strand, within PP_3948 at 4451.397 kb on - strand, within PP_3948
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,448,943 + +0.9 4,448,944 - +1.2 4,448,944 - -0.1 4,448,945 + +2.8 4,448,945 + -0.2 4,448,945 + -0.7 4,448,946 - -1.3 4,448,946 - +2.0 4,448,946 - +1.2 4,448,946 - -0.4 4,448,946 - -0.3 4,448,946 - +1.3 4,449,008 + -1.1 4,449,008 + +0.1 4,449,009 - +0.6 4,449,009 - +1.1 4,449,009 - +0.3 4,449,009 - -1.1 4,449,150 - PP_3946 0.30 -0.9 4,449,150 - PP_3946 0.30 -1.8 4,449,441 + +1.5 4,449,680 - +0.6 4,449,680 - +0.5 4,449,689 + +0.1 4,449,690 - -0.2 4,449,701 + -1.3 4,449,800 + -1.1 4,449,800 + +1.1 4,449,801 - -1.1 4,449,801 - -0.5 4,449,801 - +0.3 4,449,801 - -0.1 4,449,801 - -0.5 4,449,801 - +2.9 4,449,801 - +0.6 4,449,801 - -0.1 4,449,801 - -0.8 4,449,851 + +0.8 4,449,858 + -2.1 4,449,858 + +0.9 4,449,858 + -1.6 4,449,858 + -0.1 4,449,859 - -0.5 4,449,859 - -0.7 4,449,925 + +0.2 4,449,926 - +1.7 4,449,926 - -1.5 4,449,926 - +1.7 4,449,926 - +0.6 4,450,073 + PP_3947 0.31 -1.3 4,450,073 + PP_3947 0.31 -1.7 4,450,074 - PP_3947 0.31 +1.1 4,450,074 - PP_3947 0.31 +1.2 4,450,142 + PP_3947 0.45 +2.2 4,450,142 + PP_3947 0.45 -2.1 4,450,143 - PP_3947 0.46 -2.3 4,450,231 - PP_3947 0.64 +0.6 4,450,231 - PP_3947 0.64 -1.3 4,450,231 - PP_3947 0.64 +1.9 4,450,513 + -1.0 4,450,513 + -2.8 4,450,513 + -1.9 4,450,513 + +0.6 4,450,514 - -0.8 4,450,571 + -0.8 4,450,572 - -2.4 4,450,613 - +0.3 4,450,613 - -1.1 4,450,782 - PP_3948 0.11 +0.8 4,450,782 - PP_3948 0.11 -0.2 4,450,782 - PP_3948 0.11 +2.2 4,450,956 - PP_3948 0.16 +1.1 4,450,986 + PP_3948 0.17 +0.7 4,450,986 + PP_3948 0.17 +0.4 4,450,986 + PP_3948 0.17 -0.3 4,451,021 + PP_3948 0.18 +1.7 4,451,021 + PP_3948 0.18 +2.6 4,451,022 - PP_3948 0.18 +0.5 4,451,022 - PP_3948 0.18 -1.1 4,451,079 - PP_3948 0.19 -0.1 4,451,284 + PP_3948 0.25 -0.9 4,451,285 - PP_3948 0.25 +0.6 4,451,396 + PP_3948 0.28 -0.2 4,451,397 - PP_3948 0.28 -0.8
Or see this region's nucleotide sequence