Strain Fitness in Pseudomonas putida KT2440 around PP_3859

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,383,077	-				+0.4
4,383,093	-				-1.6
4,383,149	+		PP_3858	0.12	-0.1
4,383,149	+		PP_3858	0.12	+1.2
4,383,149	+		PP_3858	0.12	-2.0
4,383,150	-		PP_3858	0.12	-0.8
4,383,150	-		PP_3858	0.12	-0.5
4,383,193	+		PP_3858	0.18	-1.2
4,383,193	+		PP_3858	0.18	-0.6
4,383,193	+		PP_3858	0.18	+0.7
4,383,193	+		PP_3858	0.18	-0.5
4,383,194	-		PP_3858	0.18	-3.1
4,383,207	-		PP_3858	0.19	-2.1
4,383,271	+		PP_3858	0.27	+0.2
4,383,271	+		PP_3858	0.27	-0.7
4,383,271	+		PP_3858	0.27	-1.7
4,383,272	-		PP_3858	0.27	+1.2
4,383,272	-		PP_3858	0.27	-0.4
4,383,384	-		PP_3858	0.41	-1.4
4,383,385	+		PP_3858	0.42	+0.8
4,383,385	+		PP_3858	0.42	-0.2
4,383,385	+		PP_3858	0.42	+0.1
4,383,386	-		PP_3858	0.42	-0.3
4,383,386	-		PP_3858	0.42	+0.5
4,383,386	-		PP_3858	0.42	+0.5
4,383,386	-		PP_3858	0.42	-1.8
4,383,386	-		PP_3858	0.42	+0.1
4,383,404	-		PP_3858	0.44	+0.1
4,383,404	-		PP_3858	0.44	-1.0
4,383,421	+		PP_3858	0.46	-2.0
4,383,421	+		PP_3858	0.46	-0.5
4,383,421	+		PP_3858	0.46	-0.6
4,383,421	+		PP_3858	0.46	-0.4
4,383,421	+		PP_3858	0.46	+1.8
4,383,421	+		PP_3858	0.46	-1.0
4,383,421	+		PP_3858	0.46	+0.4
4,383,422	-		PP_3858	0.46	-0.8
4,383,422	-		PP_3858	0.46	-0.6
4,383,422	-		PP_3858	0.46	+0.3
4,383,440	-		PP_3858	0.48	+2.1
4,383,505	+		PP_3858	0.56	-0.8
4,383,506	-		PP_3858	0.57	-1.2
4,383,523	+		PP_3858	0.59	-0.4
4,383,568	+		PP_3858	0.64	-1.6
4,383,569	-		PP_3858	0.64	-0.4
4,383,589	+		PP_3858	0.67	-0.6
4,383,590	-		PP_3858	0.67	+1.2
4,383,706	+		PP_3858	0.81	+1.5
4,383,717	-		PP_3858	0.83	+0.1
4,383,744	-		PP_3858	0.86	-0.0
4,383,770	+		PP_3858	0.89	+1.1
4,383,771	-		PP_3858	0.90	-0.5
4,383,868	+				+0.7
4,383,868	+				+0.7
4,383,868	+				-0.9
4,383,868	+				+2.0
4,383,868	+				-0.2
4,383,869	-				+1.7
4,383,869	-				-0.5
4,383,869	-				-0.1
4,383,869	-				+1.0
4,383,869	-				-1.6
4,383,869	-				-1.5
4,383,870	+				-2.4
4,383,871	-				-2.5
4,383,897	+				-0.3
4,383,897	+				-0.9
4,383,898	-				-0.8
4,383,981	+		PP_3859	0.19	+2.0
4,383,982	-		PP_3859	0.19	-0.5
4,384,185	+		PP_3859	0.51	+0.9
4,384,185	+		PP_3859	0.51	+1.7
4,384,185	+		PP_3859	0.51	-0.7
4,384,186	-				+2.4
4,384,186	-		PP_3859	0.51	-0.5
4,384,186	-		PP_3859	0.51	-1.6
4,384,293	+		PP_3859	0.68	-1.6
4,384,293	+		PP_3859	0.68	-0.5
4,384,293	+		PP_3859	0.68	-1.8
4,384,293	+		PP_3859	0.68	-0.5
4,384,294	-		PP_3859	0.68	-0.9
4,384,294	-		PP_3859	0.68	-0.6
4,384,424	+		PP_3859	0.88	-0.6
4,384,425	-		PP_3859	0.88	+0.4
4,384,479	+				-1.4
4,384,495	+				+0.3
4,384,495	+				+1.1
4,384,518	+				-0.6
4,384,590	+				-2.1
4,384,591	-				-1.4
4,384,612	-		PP_3860	0.11	+0.3
4,384,612	-		PP_3860	0.11	+0.0
4,384,623	-		PP_3860	0.13	+0.1
4,384,757	+		PP_3860	0.25	+0.4
4,384,757	+		PP_3860	0.25	-0.3
4,384,758	-		PP_3860	0.25	-0.6
4,384,758	-		PP_3860	0.25	+0.3
4,384,758	-		PP_3860	0.25	-1.1
4,384,758	-		PP_3860	0.25	+1.3
4,384,758	-		PP_3860	0.25	-0.4
4,384,793	+		PP_3860	0.29	+1.8
4,384,793	+		PP_3860	0.29	-0.4
4,384,793	+		PP_3860	0.29	-0.2
4,384,794	-		PP_3860	0.29	-0.2
4,384,794	-		PP_3860	0.29	-0.1
4,384,873	-		PP_3860	0.36	+1.0
4,385,164	-		PP_3860	0.64	+0.6
4,385,250	+		PP_3860	0.73	+0.1
4,385,253	+		PP_3860	0.73	+0.2
4,385,253	+		PP_3860	0.73	-1.8
4,385,396	+		PP_3860	0.87	-0.4
4,385,397	-		PP_3860	0.87	-1.2
4,385,397	-		PP_3860	0.87	-0.2
4,385,405	+		PP_3860	0.87	+1.7
4,385,405	+		PP_3860	0.87	-0.4
4,385,419	-		PP_3860	0.89	-0.8