Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3823 and PP_3824 are separated by 74 nucleotides PP_3824 and PP_3825 are separated by 138 nucleotides PP_3825 and PP_5633 are separated by 7 nucleotides PP_5633 and PP_3827 are separated by 51 nucleotides
PP_3823: PP_3823 - Cytochrome c-type protein, at 4,351,096 to 4,351,749
_3823
PP_3824: PP_3824 - conserved exported protein of unknown function, at 4,351,824 to 4,352,204
_3824
PP_3825: PP_3825 - conserved protein of unknown function, at 4,352,343 to 4,352,645
_3825
PP_5633: PP_5633 - conserved protein of unknown function, at 4,352,653 to 4,352,823
_5633
PP_3827: PP_3827 - 2-nitropropane dioxygenase, at 4,352,875 to 4,353,960
_3827
Position (kb) 4352
4353 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4351.356 kb on - strand, within PP_3823 at 4351.439 kb on + strand, within PP_3823 at 4351.439 kb on + strand, within PP_3823 at 4351.440 kb on - strand, within PP_3823 at 4351.440 kb on - strand, within PP_3823 at 4351.440 kb on - strand, within PP_3823 at 4351.444 kb on - strand, within PP_3823 at 4351.547 kb on + strand, within PP_3823 at 4351.547 kb on + strand, within PP_3823 at 4351.887 kb on - strand, within PP_3824 at 4352.055 kb on + strand, within PP_3824 at 4352.055 kb on + strand, within PP_3824 at 4352.056 kb on - strand, within PP_3824 at 4352.286 kb on + strand at 4352.287 kb on - strand at 4352.287 kb on - strand at 4352.385 kb on - strand, within PP_3825 at 4352.385 kb on - strand, within PP_3825 at 4352.385 kb on - strand, within PP_3825 at 4352.471 kb on + strand, within PP_3825 at 4352.471 kb on + strand, within PP_3825 at 4352.472 kb on - strand, within PP_3825 at 4352.472 kb on - strand, within PP_3825 at 4352.476 kb on - strand, within PP_3825 at 4352.476 kb on - strand, within PP_3825 at 4352.643 kb on + strand at 4352.643 kb on + strand at 4352.643 kb on + strand at 4352.644 kb on - strand at 4352.644 kb on - strand at 4352.644 kb on - strand at 4352.671 kb on + strand, within PP_5633 at 4352.672 kb on - strand, within PP_5633 at 4352.672 kb on - strand, within PP_5633 at 4352.672 kb on - strand, within PP_5633 at 4352.672 kb on - strand, within PP_5633 at 4352.683 kb on + strand, within PP_5633 at 4352.684 kb on - strand, within PP_5633 at 4352.821 kb on + strand at 4352.898 kb on + strand at 4353.167 kb on - strand, within PP_3827 at 4353.167 kb on - strand, within PP_3827 at 4353.169 kb on + strand, within PP_3827 at 4353.169 kb on + strand, within PP_3827 at 4353.169 kb on + strand, within PP_3827 at 4353.169 kb on + strand, within PP_3827 at 4353.170 kb on - strand, within PP_3827 at 4353.170 kb on - strand, within PP_3827 at 4353.321 kb on - strand, within PP_3827 at 4353.341 kb on + strand, within PP_3827 at 4353.342 kb on - strand, within PP_3827 at 4353.342 kb on - strand, within PP_3827 at 4353.418 kb on + strand, within PP_3827 at 4353.418 kb on + strand, within PP_3827 at 4353.474 kb on - strand, within PP_3827 at 4353.491 kb on + strand, within PP_3827 at 4353.491 kb on + strand, within PP_3827 at 4353.541 kb on - strand, within PP_3827 at 4353.610 kb on + strand, within PP_3827 at 4353.611 kb on - strand, within PP_3827
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,351,356 - PP_3823 0.40 +1.3 4,351,439 + PP_3823 0.52 +0.9 4,351,439 + PP_3823 0.52 +0.8 4,351,440 - PP_3823 0.53 +1.4 4,351,440 - PP_3823 0.53 -1.6 4,351,440 - PP_3823 0.53 -0.5 4,351,444 - PP_3823 0.53 -1.3 4,351,547 + PP_3823 0.69 +2.0 4,351,547 + PP_3823 0.69 -1.5 4,351,887 - PP_3824 0.17 +1.0 4,352,055 + PP_3824 0.61 +0.7 4,352,055 + PP_3824 0.61 +2.4 4,352,056 - PP_3824 0.61 +0.4 4,352,286 + +0.8 4,352,287 - -2.7 4,352,287 - -2.2 4,352,385 - PP_3825 0.14 -0.3 4,352,385 - PP_3825 0.14 -0.8 4,352,385 - PP_3825 0.14 -0.3 4,352,471 + PP_3825 0.42 -2.2 4,352,471 + PP_3825 0.42 -0.8 4,352,472 - PP_3825 0.43 +0.5 4,352,472 - PP_3825 0.43 +1.2 4,352,476 - PP_3825 0.44 -0.1 4,352,476 - PP_3825 0.44 -0.1 4,352,643 + -2.3 4,352,643 + +0.8 4,352,643 + +0.3 4,352,644 - -1.0 4,352,644 - -0.7 4,352,644 - -0.7 4,352,671 + PP_5633 0.11 -0.3 4,352,672 - PP_5633 0.11 +0.0 4,352,672 - PP_5633 0.11 +0.4 4,352,672 - PP_5633 0.11 -0.2 4,352,672 - PP_5633 0.11 -1.1 4,352,683 + PP_5633 0.18 -1.9 4,352,684 - PP_5633 0.18 -0.4 4,352,821 + +0.1 4,352,898 + -0.3 4,353,167 - PP_3827 0.27 +1.4 4,353,167 - PP_3827 0.27 -0.4 4,353,169 + PP_3827 0.27 -0.8 4,353,169 + PP_3827 0.27 -0.8 4,353,169 + PP_3827 0.27 +0.9 4,353,169 + PP_3827 0.27 -2.5 4,353,170 - PP_3827 0.27 -2.6 4,353,170 - PP_3827 0.27 +0.2 4,353,321 - PP_3827 0.41 -0.2 4,353,341 + PP_3827 0.43 +2.3 4,353,342 - PP_3827 0.43 +1.4 4,353,342 - PP_3827 0.43 -1.7 4,353,418 + PP_3827 0.50 +0.4 4,353,418 + PP_3827 0.50 -0.4 4,353,474 - PP_3827 0.55 -1.0 4,353,491 + PP_3827 0.57 -0.3 4,353,491 + PP_3827 0.57 +0.1 4,353,541 - PP_3827 0.61 +1.5 4,353,610 + PP_3827 0.68 +0.3 4,353,611 - PP_3827 0.68 -0.4
Or see this region's nucleotide sequence