Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3800 and PP_3801 are separated by 16 nucleotides PP_3801 and PP_3802 overlap by 4 nucleotides PP_3802 and PP_3803 overlap by 1 nucleotides PP_3803 and PP_3804 overlap by 4 nucleotides
PP_3800: PP_3800 - conserved protein of unknown function, at 4,329,070 to 4,329,639
_3800
PP_3801: PP_3801 - putative Cation ABC transporter, periplasmic cation-binding protein, at 4,329,656 to 4,330,558
_3801
PP_3802: PP_3802 - putative Cation ABC transporter, ATP-binding protein, at 4,330,555 to 4,331,301
_3802
PP_3803: PP_3803 - putative Cation ABC transporter, permease protein, at 4,331,301 to 4,332,200
_3803
PP_3804: PP_3804 - conserved exported protein of unknown function, at 4,332,197 to 4,333,060
_3804
Position (kb)
4330
4331
4332 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4329.604 kb on + strand at 4329.719 kb on - strand at 4329.758 kb on + strand, within PP_3801 at 4329.759 kb on - strand, within PP_3801 at 4329.759 kb on - strand, within PP_3801 at 4329.761 kb on + strand, within PP_3801 at 4329.762 kb on - strand, within PP_3801 at 4329.762 kb on - strand, within PP_3801 at 4329.762 kb on - strand, within PP_3801 at 4329.767 kb on + strand, within PP_3801 at 4329.767 kb on + strand, within PP_3801 at 4329.768 kb on - strand, within PP_3801 at 4329.768 kb on - strand, within PP_3801 at 4329.768 kb on - strand, within PP_3801 at 4329.778 kb on + strand, within PP_3801 at 4329.778 kb on + strand, within PP_3801 at 4329.779 kb on - strand, within PP_3801 at 4329.779 kb on - strand, within PP_3801 at 4329.840 kb on - strand, within PP_3801 at 4330.133 kb on + strand, within PP_3801 at 4330.133 kb on + strand, within PP_3801 at 4330.251 kb on - strand, within PP_3801 at 4330.256 kb on + strand, within PP_3801 at 4330.256 kb on + strand, within PP_3801 at 4330.257 kb on - strand, within PP_3801 at 4330.409 kb on + strand, within PP_3801 at 4330.410 kb on - strand, within PP_3801 at 4330.462 kb on + strand, within PP_3801 at 4330.462 kb on + strand, within PP_3801 at 4330.462 kb on + strand, within PP_3801 at 4330.463 kb on - strand, within PP_3801 at 4330.463 kb on - strand, within PP_3801 at 4330.463 kb on - strand, within PP_3801 at 4330.478 kb on + strand at 4330.479 kb on - strand at 4330.493 kb on + strand at 4330.493 kb on + strand at 4330.493 kb on + strand at 4330.494 kb on - strand at 4330.494 kb on - strand at 4330.494 kb on - strand at 4330.801 kb on + strand, within PP_3802 at 4330.801 kb on + strand, within PP_3802 at 4330.802 kb on - strand, within PP_3802 at 4331.105 kb on + strand, within PP_3802 at 4331.105 kb on + strand, within PP_3802 at 4331.106 kb on - strand, within PP_3802 at 4331.106 kb on - strand, within PP_3802 at 4331.133 kb on + strand, within PP_3802 at 4331.352 kb on + strand at 4331.352 kb on + strand at 4331.353 kb on - strand at 4331.353 kb on - strand at 4331.373 kb on + strand at 4331.373 kb on + strand at 4331.374 kb on - strand at 4331.374 kb on - strand at 4331.535 kb on + strand, within PP_3803 at 4331.535 kb on + strand, within PP_3803 at 4331.535 kb on + strand, within PP_3803 at 4331.535 kb on + strand, within PP_3803 at 4331.535 kb on + strand at 4331.538 kb on - strand, within PP_3803 at 4331.538 kb on - strand, within PP_3803 at 4331.610 kb on - strand, within PP_3803 at 4331.627 kb on + strand, within PP_3803 at 4331.750 kb on + strand, within PP_3803 at 4331.817 kb on + strand, within PP_3803 at 4331.818 kb on - strand, within PP_3803 at 4331.892 kb on + strand, within PP_3803 at 4331.893 kb on - strand, within PP_3803
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,329,604 + -0.1 4,329,719 - -0.4 4,329,758 + PP_3801 0.11 -2.1 4,329,759 - PP_3801 0.11 -0.5 4,329,759 - PP_3801 0.11 -0.5 4,329,761 + PP_3801 0.12 +0.4 4,329,762 - PP_3801 0.12 -1.3 4,329,762 - PP_3801 0.12 -0.1 4,329,762 - PP_3801 0.12 -0.2 4,329,767 + PP_3801 0.12 -0.3 4,329,767 + PP_3801 0.12 -0.7 4,329,768 - PP_3801 0.12 -0.3 4,329,768 - PP_3801 0.12 -0.3 4,329,768 - PP_3801 0.12 -0.4 4,329,778 + PP_3801 0.14 -0.2 4,329,778 + PP_3801 0.14 -0.3 4,329,779 - PP_3801 0.14 +0.6 4,329,779 - PP_3801 0.14 -1.7 4,329,840 - PP_3801 0.20 -0.9 4,330,133 + PP_3801 0.53 -0.3 4,330,133 + PP_3801 0.53 +0.6 4,330,251 - PP_3801 0.66 +0.2 4,330,256 + PP_3801 0.66 +0.5 4,330,256 + PP_3801 0.66 -0.3 4,330,257 - PP_3801 0.67 -1.4 4,330,409 + PP_3801 0.83 -0.1 4,330,410 - PP_3801 0.83 -1.1 4,330,462 + PP_3801 0.89 -0.1 4,330,462 + PP_3801 0.89 +0.6 4,330,462 + PP_3801 0.89 -0.2 4,330,463 - PP_3801 0.89 -0.1 4,330,463 - PP_3801 0.89 -0.2 4,330,463 - PP_3801 0.89 -1.7 4,330,478 + -1.3 4,330,479 - +2.2 4,330,493 + -0.2 4,330,493 + +0.8 4,330,493 + +0.0 4,330,494 - -0.7 4,330,494 - -0.8 4,330,494 - +1.5 4,330,801 + PP_3802 0.33 -1.5 4,330,801 + PP_3802 0.33 -0.9 4,330,802 - PP_3802 0.33 +0.4 4,331,105 + PP_3802 0.74 +0.5 4,331,105 + PP_3802 0.74 -2.6 4,331,106 - PP_3802 0.74 +0.4 4,331,106 - PP_3802 0.74 -1.2 4,331,133 + PP_3802 0.77 -1.8 4,331,352 + +1.1 4,331,352 + +0.8 4,331,353 - +0.2 4,331,353 - -1.9 4,331,373 + -0.1 4,331,373 + -1.1 4,331,374 - -0.8 4,331,374 - +0.5 4,331,535 + PP_3803 0.26 -0.2 4,331,535 + PP_3803 0.26 -2.2 4,331,535 + PP_3803 0.26 -0.2 4,331,535 + PP_3803 0.26 -0.0 4,331,535 + +0.5 4,331,538 - PP_3803 0.26 -0.1 4,331,538 - PP_3803 0.26 -1.8 4,331,610 - PP_3803 0.34 +0.1 4,331,627 + PP_3803 0.36 +0.7 4,331,750 + PP_3803 0.50 +0.5 4,331,817 + PP_3803 0.57 -1.6 4,331,818 - PP_3803 0.57 -1.3 4,331,892 + PP_3803 0.66 -0.5 4,331,893 - PP_3803 0.66 +1.1
Or see this region's nucleotide sequence