Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3755 and PP_3756 are separated by 12 nucleotides PP_3756 and PP_3757 are separated by 48 nucleotides PP_3757 and PP_3758 are separated by 44 nucleotides
PP_3755: PP_3755 - 3-hydroxybutyryl-CoA dehydrogenase, at 4,284,596 to 4,285,444
_3755
PP_3756: PP_3756 - TetR family transcriptional regulator, at 4,285,457 to 4,286,038
_3756
PP_3757: PP_3757 - Chemotaxis protein CheY, at 4,286,087 to 4,286,464
_3757
PP_3758: PP_3758 - Response regulator/sensor histidine kinase, at 4,286,509 to 4,287,735
_3758
Position (kb)
4286
4287 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4285.362 kb on - strand at 4285.440 kb on - strand at 4285.440 kb on - strand at 4285.601 kb on + strand, within PP_3756 at 4285.601 kb on + strand, within PP_3756 at 4285.634 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.655 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.847 kb on + strand, within PP_3756 at 4285.924 kb on - strand, within PP_3756 at 4286.123 kb on - strand at 4286.258 kb on - strand, within PP_3757 at 4286.373 kb on + strand, within PP_3757 at 4286.374 kb on - strand at 4286.391 kb on + strand, within PP_3757 at 4286.391 kb on + strand, within PP_3757 at 4286.484 kb on + strand at 4286.484 kb on + strand at 4286.484 kb on + strand at 4286.484 kb on + strand at 4286.484 kb on + strand at 4286.485 kb on - strand at 4286.510 kb on + strand at 4286.511 kb on - strand at 4286.651 kb on + strand, within PP_3758 at 4286.651 kb on + strand, within PP_3758 at 4286.651 kb on + strand, within PP_3758 at 4286.651 kb on + strand, within PP_3758 at 4286.652 kb on - strand, within PP_3758 at 4286.652 kb on - strand, within PP_3758 at 4286.652 kb on - strand, within PP_3758 at 4286.652 kb on - strand, within PP_3758 at 4286.652 kb on - strand, within PP_3758 at 4286.854 kb on + strand, within PP_3758 at 4286.854 kb on + strand, within PP_3758 at 4286.855 kb on - strand, within PP_3758 at 4286.855 kb on - strand, within PP_3758 at 4286.855 kb on - strand, within PP_3758 at 4287.193 kb on - strand, within PP_3758 at 4287.437 kb on + strand, within PP_3758 at 4287.453 kb on - strand, within PP_3758
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,285,362 - +1.0 4,285,440 - +2.4 4,285,440 - -0.0 4,285,601 + PP_3756 0.25 +0.1 4,285,601 + PP_3756 0.25 -1.6 4,285,634 + PP_3756 0.30 -1.8 4,285,655 + PP_3756 0.34 -1.1 4,285,655 + PP_3756 0.34 +0.4 4,285,655 + PP_3756 0.34 -1.3 4,285,847 + PP_3756 0.67 -1.6 4,285,847 + PP_3756 0.67 -0.9 4,285,847 + PP_3756 0.67 +1.0 4,285,924 - PP_3756 0.80 -1.2 4,286,123 - +0.2 4,286,258 - PP_3757 0.45 -1.3 4,286,373 + PP_3757 0.76 -2.1 4,286,374 - +2.4 4,286,391 + PP_3757 0.80 +0.0 4,286,391 + PP_3757 0.80 -0.2 4,286,484 + -0.3 4,286,484 + +0.0 4,286,484 + +1.1 4,286,484 + +0.4 4,286,484 + +0.1 4,286,485 - +1.1 4,286,510 + -2.3 4,286,511 - -1.2 4,286,651 + PP_3758 0.12 -0.7 4,286,651 + PP_3758 0.12 +1.3 4,286,651 + PP_3758 0.12 +4.7 4,286,651 + PP_3758 0.12 +0.7 4,286,652 - PP_3758 0.12 +0.4 4,286,652 - PP_3758 0.12 -0.6 4,286,652 - PP_3758 0.12 -0.3 4,286,652 - PP_3758 0.12 -2.0 4,286,652 - PP_3758 0.12 -1.7 4,286,854 + PP_3758 0.28 -1.4 4,286,854 + PP_3758 0.28 -0.9 4,286,855 - PP_3758 0.28 +1.4 4,286,855 - PP_3758 0.28 -1.0 4,286,855 - PP_3758 0.28 -0.4 4,287,193 - PP_3758 0.56 +1.1 4,287,437 + PP_3758 0.76 +0.5 4,287,453 - PP_3758 0.77 +0.2
Or see this region's nucleotide sequence