Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3724 and PP_3725 are separated by 17 nucleotides PP_3725 and PP_3726 are separated by 33 nucleotides
PP_3724: PP_3724 - putative Acyl-CoA synthetase, at 4,247,909 to 4,250,026
_3724
PP_3725: PP_3725 - putative Acyl-CoA dehydrogenase, at 4,250,044 to 4,251,204
_3725
PP_3726: PP_3726 - Enoyl-CoA hydratase/isomerase family protein, at 4,251,238 to 4,252,032
_3726
Position (kb)
4250
4251
4252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4249.237 kb on - strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.488 kb on + strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.528 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4249.735 kb on - strand, within PP_3724 at 4250.083 kb on - strand at 4250.083 kb on - strand at 4250.364 kb on - strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.567 kb on + strand, within PP_3725 at 4250.576 kb on + strand, within PP_3725 at 4250.577 kb on - strand at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.684 kb on + strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand, within PP_3725 at 4250.685 kb on - strand at 4250.879 kb on + strand, within PP_3725 at 4250.879 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.996 kb on + strand, within PP_3725 at 4250.997 kb on - strand, within PP_3725 at 4251.041 kb on + strand, within PP_3725 at 4251.041 kb on + strand, within PP_3725 at 4251.041 kb on + strand, within PP_3725 at 4251.041 kb on + strand, within PP_3725 at 4251.042 kb on - strand, within PP_3725 at 4251.239 kb on + strand at 4251.379 kb on + strand, within PP_3726 at 4251.783 kb on - strand, within PP_3726 at 4252.119 kb on + strand at 4252.119 kb on + strand at 4252.120 kb on - strand at 4252.150 kb on - strand at 4252.150 kb on - strand at 4252.178 kb on - strand at 4252.178 kb on - strand at 4252.203 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,249,237 - PP_3724 0.63 +0.5 4,249,488 + PP_3724 0.75 -0.2 4,249,488 + PP_3724 0.75 +0.6 4,249,528 - PP_3724 0.76 +0.2 4,249,528 - PP_3724 0.76 -1.6 4,249,735 - PP_3724 0.86 +1.0 4,249,735 - PP_3724 0.86 +1.6 4,249,735 - PP_3724 0.86 +0.4 4,249,735 - PP_3724 0.86 -0.3 4,250,083 - +0.1 4,250,083 - -1.9 4,250,364 - PP_3725 0.28 -0.6 4,250,567 + PP_3725 0.45 -0.8 4,250,567 + PP_3725 0.45 +1.2 4,250,567 + PP_3725 0.45 +1.6 4,250,576 + PP_3725 0.46 -1.3 4,250,577 - +2.4 4,250,684 + PP_3725 0.55 +0.8 4,250,684 + PP_3725 0.55 -0.7 4,250,684 + PP_3725 0.55 +1.1 4,250,685 - PP_3725 0.55 +0.2 4,250,685 - PP_3725 0.55 +1.1 4,250,685 - PP_3725 0.55 -0.6 4,250,685 - PP_3725 0.55 -0.2 4,250,685 - +1.9 4,250,879 + PP_3725 0.72 +1.8 4,250,879 + PP_3725 0.72 +0.6 4,250,996 + PP_3725 0.82 -0.2 4,250,996 + PP_3725 0.82 +0.4 4,250,997 - PP_3725 0.82 -0.9 4,251,041 + PP_3725 0.86 -0.8 4,251,041 + PP_3725 0.86 +1.4 4,251,041 + PP_3725 0.86 +1.2 4,251,041 + PP_3725 0.86 +0.1 4,251,042 - PP_3725 0.86 +0.5 4,251,239 + +1.6 4,251,379 + PP_3726 0.18 -1.6 4,251,783 - PP_3726 0.69 +1.0 4,252,119 + -0.9 4,252,119 + -0.9 4,252,120 - -1.2 4,252,150 - +0.6 4,252,150 - +0.1 4,252,178 - -0.8 4,252,178 - -0.2 4,252,203 + +1.2
Or see this region's nucleotide sequence