Strain Fitness in Pseudomonas putida KT2440 around PP_3663

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,159,756	+		PP_3662	0.36	-0.2
4,159,756	+		PP_3662	0.36	-0.9
4,159,756	+		PP_3662	0.36	-1.1
4,159,757	-		PP_3662	0.36	-0.5
4,159,763	-		PP_3662	0.37	+0.1
4,159,763	-		PP_3662	0.37	-0.9
4,159,763	-		PP_3662	0.37	+0.5
4,160,065	+		PP_3662	0.59	-0.2
4,160,065	+		PP_3662	0.59	+0.7
4,160,066	-		PP_3662	0.59	+1.2
4,160,066	-		PP_3662	0.59	+0.7
4,160,149	+		PP_3662	0.65	+1.4
4,160,150	-		PP_3662	0.65	-0.1
4,160,210	-		PP_3662	0.69	-0.0
4,160,531	+				+1.8
4,160,531	+				-0.7
4,160,605	+				-0.6
4,160,605	+				+2.0
4,160,605	+				-1.4
4,160,606	-				-2.1
4,160,609	+				+0.2
4,160,610	-				-1.3
4,160,617	+				-0.1
4,160,617	+				-0.6
4,160,617	+				-1.2
4,160,618	-				-0.6
4,160,618	-				-2.1
4,160,703	+				+0.4
4,160,723	+				-1.3
4,160,723	+				+0.2
4,160,723	+				+2.0
4,160,723	+				-2.0
4,160,724	-				-0.6
4,160,724	-				+1.0
4,160,724	-				+0.9
4,160,724	-				-0.3
4,160,743	+				+0.4
4,160,743	+				-0.1
4,160,744	-				+0.0
4,160,744	-				+0.1
4,160,744	-				-0.2
4,160,825	+				-0.8
4,160,923	-		PP_3663	0.13	+0.5
4,160,943	-		PP_3663	0.14	+1.4
4,161,114	+		PP_3663	0.28	+0.1
4,161,239	+		PP_3663	0.37	-0.4
4,161,240	-		PP_3663	0.37	-1.4
4,161,301	+		PP_3663	0.42	-0.9
4,161,301	+		PP_3663	0.42	-1.2
4,161,302	-		PP_3663	0.42	-2.1
4,161,302	-		PP_3663	0.42	-0.2
4,161,440	+		PP_3663	0.53	+0.7
4,161,440	+		PP_3663	0.53	+1.0
4,161,440	+		PP_3663	0.53	+0.2
4,161,440	+		PP_3663	0.53	-1.0
4,161,440	+		PP_3663	0.53	-1.3
4,161,441	-		PP_3663	0.53	+0.1
4,161,467	+		PP_3663	0.55	+0.0
4,161,468	-		PP_3663	0.55	-0.5
4,161,468	-		PP_3663	0.55	-1.3
4,161,468	-		PP_3663	0.55	-0.1
4,161,732	+		PP_3663	0.75	-2.2
4,161,777	+		PP_3663	0.78	-1.2
4,161,777	+		PP_3663	0.78	+1.0
4,161,778	-		PP_3663	0.79	+0.1
4,161,778	-		PP_3663	0.79	+2.2
4,161,778	-		PP_3663	0.79	+0.3
4,161,813	-		PP_3663	0.81	-0.4
4,161,813	-		PP_3663	0.81	-1.0
4,161,857	+		PP_3663	0.85	-0.7
4,161,858	-		PP_3663	0.85	-0.6
4,161,891	+		PP_3663	0.87	-0.0
4,161,891	+		PP_3663	0.87	-0.1
4,161,914	-		PP_3663	0.89	-0.4
4,161,914	-		PP_3663	0.89	+0.5
4,161,921	+		PP_3663	0.90	-0.7
4,161,921	+		PP_3663	0.90	-1.3
4,161,922	-		PP_3663	0.90	-1.5
4,161,922	-		PP_3663	0.90	-0.8
4,161,923	+		PP_3663	0.90	+1.5
4,161,923	+		PP_3663	0.90	+0.4
4,161,923	+		PP_3663	0.90	+0.2
4,161,924	-		PP_3663	0.90	+0.1
4,161,964	+				+2.8
4,161,965	-				+0.6
4,161,965	-				-2.0
4,161,965	-				+1.1
4,162,016	+				-0.4
4,162,017	-				-1.2
4,162,017	-				+0.9
4,162,052	-				+0.2
4,162,057	-				-0.6
4,162,122	+				-1.5
4,162,122	+				-1.0
4,162,123	-				-1.3
4,162,171	-				+0.3
4,162,265	+				+0.4
4,162,265	+				+0.3
4,162,265	+				-0.0
4,162,266	-		PP_3664	0.10	+0.7
4,162,266	-		PP_3664	0.10	-1.3
4,162,266	-		PP_3664	0.10	-0.3
4,162,266	-		PP_3664	0.10	-1.2
4,162,266	-				+1.2
4,162,427	-		PP_3664	0.22	-0.6
4,162,427	-		PP_3664	0.22	+0.9
4,162,427	-		PP_3664	0.22	-1.1
4,162,451	+		PP_3664	0.24	-0.1
4,162,452	-		PP_3664	0.24	+0.2
4,162,452	-		PP_3664	0.24	+1.3
4,162,519	-		PP_3664	0.29	-0.4
4,162,519	-		PP_3664	0.29	+0.4
4,162,524	+		PP_3664	0.29	+0.2
4,162,524	+		PP_3664	0.29	-0.4
4,162,524	+		PP_3664	0.29	-1.5
4,162,524	+		PP_3664	0.29	-2.0
4,162,525	-		PP_3664	0.29	-0.2
4,162,525	-				+1.1
4,162,569	+		PP_3664	0.33	-0.9
4,162,570	-		PP_3664	0.33	+1.1
4,162,570	-		PP_3664	0.33	-0.1
4,162,570	-		PP_3664	0.33	-1.5
4,162,672	-		PP_3664	0.41	+0.3
4,162,678	+		PP_3664	0.41	-0.3
4,162,678	+		PP_3664	0.41	-0.6
4,162,678	+		PP_3664	0.41	-1.0
4,162,810	-		PP_3664	0.51	+0.4
4,162,868	-		PP_3664	0.55	-0.2
4,162,906	+		PP_3664	0.58	+0.4
4,162,974	+		PP_3664	0.63	+0.3
4,163,010	+		PP_3664	0.66	+0.6
4,163,011	-		PP_3664	0.66	+0.4
4,163,037	+		PP_3664	0.68	-0.0
4,163,038	-				+0.9