Strain Fitness in Pseudomonas putida KT2440 around PP_3576

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,056,826	+		PP_3575	0.65	+1.9
4,056,826	+		PP_3575	0.65	-0.9
4,056,826	+		PP_3575	0.65	+0.2
4,056,983	-				+1.1
4,057,000	+		PP_3575	0.72	+0.1
4,057,000	+		PP_3575	0.72	-1.0
4,057,085	+		PP_3575	0.76	-0.9
4,057,162	+		PP_3575	0.79	-1.2
4,057,163	-		PP_3575	0.79	+0.8
4,057,249	+		PP_3575	0.83	-1.3
4,057,420	+		PP_3575	0.90	-1.2
4,057,439	+				-1.7
4,057,440	-				-0.5
4,057,455	+				-1.6
4,057,456	-				-0.1
4,057,456	-				+0.2
4,057,456	-				-1.2
4,057,547	+				-1.7
4,057,650	+				+0.9
4,057,651	-				-0.4
4,057,651	-				-2.2
4,057,904	+		PP_3576	0.19	-0.8
4,058,047	+		PP_3576	0.34	-1.5
4,058,047	+		PP_3576	0.34	-0.0
4,058,047	+		PP_3576	0.34	-1.5
4,058,048	-		PP_3576	0.34	-2.2
4,058,048	-		PP_3576	0.34	-0.8
4,058,048	-		PP_3576	0.34	+1.0
4,058,702	-				+0.7
4,058,716	+				+2.3
4,058,717	-				-0.8
4,058,717	-				-0.5
4,058,717	-				+0.4
4,058,717	-				-1.7
4,058,926	+		PP_3577	0.50	+0.8
4,058,926	+		PP_3577	0.50	-0.1
4,058,926	+		PP_3577	0.50	+0.6
4,058,926	+		PP_3577	0.50	+0.3
4,058,927	-		PP_3577	0.50	-1.0
4,058,942	+		PP_3577	0.53	+1.4
4,058,942	+		PP_3577	0.53	-0.9
4,058,943	-		PP_3577	0.53	-1.4
4,058,992	+		PP_3577	0.63	+0.4
4,059,009	+		PP_3577	0.66	-0.7
4,059,054	-		PP_3577	0.75	-0.9
4,059,092	+		PP_3577	0.82	-0.5
4,059,093	-		PP_3577	0.82	-2.0
4,059,093	-		PP_3577	0.82	+0.5
4,059,137	-				+0.1
4,059,352	+				-0.1
4,059,352	+				+0.6
4,059,353	-				+0.6
4,059,353	-				+0.2
4,059,410	+				-1.3
4,059,410	+				-0.2
4,059,410	+				+1.3
4,059,411	-				-0.6
4,059,598	+		PP_3578	0.21	-2.7
4,059,599	-		PP_3578	0.21	-0.8
4,059,599	-		PP_3578	0.21	-1.0