Strain Fitness in Pseudomonas putida KT2440 around PP_3559

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
4,037,281	+				+1.0
4,037,512	+		PP_3558	0.32	+0.3
4,037,552	+		PP_3558	0.36	+0.2
4,037,552	+		PP_3558	0.36	-1.4
4,037,552	+		PP_3558	0.36	-1.5
4,037,552	+		PP_3558	0.36	-0.1
4,037,736	-		PP_3558	0.54	-0.8
4,037,753	+		PP_3558	0.55	-0.6
4,037,754	-		PP_3558	0.56	-1.0
4,037,754	-		PP_3558	0.56	+1.4
4,037,754	-		PP_3558	0.56	-2.0
4,037,754	-		PP_3558	0.56	-1.0
4,037,754	-		PP_3558	0.56	+1.3
4,037,838	-		PP_3558	0.64	-1.6
4,037,838	-		PP_3558	0.64	-1.8
4,037,838	-		PP_3558	0.64	+1.0
4,037,870	+		PP_3558	0.67	+1.3
4,038,014	+		PP_3558	0.81	+0.9
4,038,015	-		PP_3558	0.81	+0.1
4,038,045	+		PP_3558	0.84	-0.5
4,038,046	-		PP_3558	0.84	+0.0
4,038,275	+				+0.7
4,038,276	-				+0.0
4,038,276	-				-0.5
4,038,284	-				-1.4
4,038,542	+		PP_3559	0.40	-2.3
4,038,543	-		PP_3559	0.40	-0.2
4,038,634	+		PP_3559	0.51	-0.2
4,038,700	+		PP_3559	0.59	-0.9
4,038,700	+		PP_3559	0.59	-1.6
4,038,701	-		PP_3559	0.59	+1.1
4,039,030	+				-0.4
4,039,038	+				+0.6
4,039,039	-				-1.4
4,039,039	-				-0.3
4,039,109	-				-0.2
4,039,109	-				-0.4
4,039,310	+		PP_3560	0.19	-0.6
4,039,367	-		PP_3560	0.26	-1.0
4,039,367	-		PP_3560	0.26	+0.4
4,039,499	+		PP_3560	0.41	-0.1
4,039,499	+		PP_3560	0.41	-0.2
4,039,500	-		PP_3560	0.41	-1.2
4,039,500	-		PP_3560	0.41	-0.4
4,039,674	+		PP_3560	0.61	+0.7
4,039,757	+		PP_3560	0.71	+0.0