Strain Fitness in Pseudomonas putida KT2440 around PP_3529

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,999,371	+				-0.3
3,999,560	+		PP_3528	0.25	+1.2
3,999,561	-		PP_3528	0.25	-0.9
3,999,674	-		PP_3528	0.36	-0.4
3,999,778	+		PP_3528	0.47	-0.1
3,999,792	-		PP_3528	0.48	+0.4
3,999,817	+		PP_3528	0.51	-2.0
3,999,818	-		PP_3528	0.51	-2.3
3,999,828	+		PP_3528	0.52	-1.9
3,999,838	+		PP_3528	0.53	+0.4
3,999,991	+		PP_3528	0.68	+0.6
3,999,991	+		PP_3528	0.68	-0.4
3,999,991	+		PP_3528	0.68	+0.4
3,999,992	-		PP_3528	0.68	+0.0
3,999,992	-		PP_3528	0.68	-1.4
3,999,992	-		PP_3528	0.68	-0.4
3,999,992	-		PP_3528	0.68	-0.5
3,999,992	-		PP_3528	0.68	-0.5
3,999,992	-		PP_3528	0.68	+0.5
4,000,168	+		PP_3528	0.86	-0.1
4,000,183	-		PP_3528	0.88	-1.9
4,000,259	+				-1.6
4,000,260	-				-1.0
4,000,260	-				-1.1
4,000,430	+				+0.3
4,000,431	-				+0.2
4,000,431	-				+0.3
4,000,505	+		PP_3529	0.14	-0.9
4,000,505	+		PP_3529	0.14	-0.8
4,000,505	+		PP_3529	0.14	+0.2
4,000,506	-		PP_3529	0.14	+0.1
4,000,506	-		PP_3529	0.14	-2.3
4,000,506	-		PP_3529	0.14	-1.7
4,000,506	-		PP_3529	0.14	+0.3
4,000,515	-		PP_3529	0.14	-0.8
4,000,643	+		PP_3529	0.24	+1.1
4,000,644	-		PP_3529	0.24	-0.4
4,000,644	-		PP_3529	0.24	-1.4
4,000,644	-		PP_3529	0.24	+0.7
4,000,730	+		PP_3529	0.30	-0.2
4,000,790	+		PP_3529	0.35	-0.6
4,000,808	+		PP_3529	0.36	+0.2
4,000,808	+		PP_3529	0.36	-1.0
4,000,809	-		PP_3529	0.36	-1.4
4,000,809	-		PP_3529	0.36	+0.7
4,000,823	+		PP_3529	0.37	+0.1
4,000,823	+		PP_3529	0.37	+0.8
4,000,823	+		PP_3529	0.37	+0.7
4,000,824	-		PP_3529	0.37	-0.2
4,000,824	-		PP_3529	0.37	-1.9
4,000,886	+		PP_3529	0.42	+0.1
4,000,887	-		PP_3529	0.42	-0.4
4,000,887	-		PP_3529	0.42	-1.0
4,000,887	-		PP_3529	0.42	-2.6
4,000,888	+		PP_3529	0.42	-0.6
4,001,207	-				+1.6
4,001,214	-		PP_3529	0.66	-1.3
4,001,214	-		PP_3529	0.66	+0.6
4,001,222	+		PP_3529	0.66	-2.6
4,001,309	+		PP_3529	0.73	-0.1
4,001,309	+		PP_3529	0.73	-3.3
4,001,310	-		PP_3529	0.73	-2.9
4,001,382	+		PP_3529	0.78	+0.3
4,001,383	-		PP_3529	0.78	+0.5
4,001,511	-		PP_3529	0.87	+0.7
4,001,541	+		PP_3529	0.90	-1.0
4,001,541	+		PP_3529	0.90	+0.2
4,001,595	-				+1.2
4,001,736	+				+0.4
4,001,736	+				-0.6
4,001,832	+				+0.1
4,001,905	-		PP_3530	0.11	+1.3
4,001,905	-		PP_3530	0.11	-1.5
4,002,028	-		PP_3530	0.25	+0.2
4,002,028	-		PP_3530	0.25	+0.7
4,002,250	-		PP_3530	0.52	+1.2
4,002,465	+		PP_3530	0.77	-0.7
4,002,466	-		PP_3530	0.78	+0.4
4,002,466	-		PP_3530	0.78	-1.4
4,002,466	-		PP_3530	0.78	-0.4
4,002,590	+				-0.7
4,002,590	+				-0.7
4,002,591	-				+0.6
4,002,591	-				-0.9
4,002,591	-				-0.3
4,002,591	-				-0.8
4,002,594	+				-2.0
4,002,603	+				-0.2
4,002,603	+				-0.2
4,002,603	+				+1.9
4,002,604	-				+0.2
4,002,625	+				-0.2
4,002,625	+				-0.1
4,002,625	+				+1.2
4,002,625	+				-0.6
4,002,626	-				-0.1
4,002,626	-				-0.0
4,002,626	-				+0.4
4,002,626	-				-0.2
4,002,652	-				-0.2