Strain Fitness in Pseudomonas putida KT2440 around PP_3446

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
3,902,334	+		PP_3445	0.42	+0.4
3,902,335	-		PP_3445	0.42	-0.4
3,902,551	+		PP_3445	0.60	-0.2
3,902,552	-		PP_3445	0.60	-0.4
3,902,552	-		PP_3445	0.60	-2.1
3,902,664	+		PP_3445	0.69	+0.5
3,902,672	+		PP_3445	0.70	+0.5
3,902,694	+		PP_3445	0.72	-0.3
3,902,755	-		PP_3445	0.77	-0.9
3,902,816	+		PP_3445	0.82	-1.6
3,902,817	-		PP_3445	0.82	-0.4
3,902,967	+				+0.2
3,903,169	+				-0.3
3,903,170	-				-0.5
3,903,390	+		PP_3446	0.15	-0.7
3,903,391	-				+0.8
3,903,799	+		PP_3446	0.41	+0.1
3,903,800	-				+1.6
3,903,800	-		PP_3446	0.41	+0.6
3,903,800	-		PP_3446	0.41	-1.2
3,903,800	-		PP_3446	0.41	-1.3
3,903,863	-		PP_3446	0.45	+0.6
3,903,867	-		PP_3446	0.45	-0.2
3,903,867	-		PP_3446	0.45	-0.9
3,904,090	+		PP_3446	0.59	+0.4
3,904,090	+		PP_3446	0.59	-0.1
3,904,090	+		PP_3446	0.59	-0.9
3,904,091	-		PP_3446	0.59	-2.5
3,904,099	+		PP_3446	0.60	-2.2
3,904,099	+		PP_3446	0.60	-2.6
3,904,099	+		PP_3446	0.60	+1.1
3,904,099	+		PP_3446	0.60	-0.7
3,904,100	-		PP_3446	0.60	+0.1
3,904,183	+		PP_3446	0.65	-2.1
3,904,499	-		PP_3446	0.85	-2.1
3,904,526	+		PP_3446	0.86	-0.8
3,904,622	-				+0.6
3,904,683	-				+1.0
3,904,685	+				+0.3
3,904,686	-				-2.1
3,904,731	+				+0.1
3,904,731	+				-1.9
3,904,732	-				-1.2
3,904,788	+				-0.2
3,904,823	+				+0.8
3,904,823	+				-0.8
3,904,823	+				-0.7
3,904,824	-				-2.4
3,904,826	+				+0.4
3,904,827	-				-1.0
3,904,827	-				-1.7
3,904,827	-				-1.1
3,904,850	+				-1.6
3,904,851	-				-0.7
3,904,851	-				-1.4
3,904,871	-				-0.8
3,905,161	-		PP_3447	0.62	+1.1
3,905,350	+				+0.6
3,905,356	+				+0.8
3,905,356	+				+0.0
3,905,357	-				+0.6
3,905,417	+				-0.2
3,905,417	+				+0.0
3,905,417	+				-0.2
3,905,417	+				-0.7
3,905,417	+				+1.8
3,905,418	-				-1.5
3,905,418	-				+0.2
3,905,421	+				+1.8
3,905,422	-				-0.6
3,905,428	+				-0.6
3,905,428	+				-0.6
3,905,429	-				-1.6
3,905,429	-				+1.0
3,905,429	-				+3.5
3,905,477	+				+1.8
3,905,527	-				-0.0
3,905,546	+				-0.2
3,905,547	-				-0.9
3,905,637	-				-1.5
3,905,637	-				+0.8
3,905,637	-				-0.3
3,905,637	-				+0.1
3,905,672	+				+0.2
3,905,708	+				-1.1
3,905,708	+				-0.3
3,905,708	+				+1.4
3,905,709	-				-0.3