Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3403 and PP_3404 are separated by 96 nucleotides PP_3404 and PP_3405 are separated by 21 nucleotides PP_3405 and PP_3406 are separated by 124 nucleotides PP_3406 and PP_3407 overlap by 4 nucleotides
PP_3403: PP_3403 - putative Quercetin 2,3-dioxygenase, at 3,854,315 to 3,855,202
_3403
PP_3404: PP_3404 - conserved protein of unknown function, at 3,855,299 to 3,856,171
_3404
PP_3405: PP_3405 - conserved membrane protein of unknown function, at 3,856,193 to 3,856,822
_3405
PP_3406: PP_3406 - Acetyltransferase, GNAT family, at 3,856,947 to 3,857,426
_3406
PP_3407: PP_3407 - conserved protein of unknown function, at 3,857,423 to 3,857,653
_3407
Position (kb)
3856
3857 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3855.441 kb on + strand at 3855.441 kb on + strand, within PP_3404 at 3855.441 kb on + strand, within PP_3404 at 3855.442 kb on - strand, within PP_3404 at 3855.456 kb on + strand, within PP_3404 at 3855.456 kb on + strand, within PP_3404 at 3855.457 kb on - strand, within PP_3404 at 3855.597 kb on + strand, within PP_3404 at 3855.598 kb on - strand, within PP_3404 at 3855.598 kb on - strand, within PP_3404 at 3856.047 kb on + strand, within PP_3404 at 3856.089 kb on - strand at 3856.178 kb on - strand at 3856.183 kb on - strand at 3856.183 kb on - strand at 3856.388 kb on + strand, within PP_3405 at 3856.388 kb on + strand, within PP_3405 at 3856.389 kb on - strand, within PP_3405 at 3856.389 kb on - strand, within PP_3405 at 3856.389 kb on - strand, within PP_3405 at 3856.392 kb on + strand, within PP_3405 at 3856.392 kb on + strand, within PP_3405 at 3856.392 kb on + strand, within PP_3405 at 3856.392 kb on + strand, within PP_3405 at 3856.393 kb on - strand, within PP_3405 at 3856.393 kb on - strand, within PP_3405 at 3856.393 kb on - strand, within PP_3405 at 3856.393 kb on - strand, within PP_3405 at 3856.393 kb on - strand, within PP_3405 at 3856.410 kb on + strand, within PP_3405 at 3856.410 kb on + strand, within PP_3405 at 3856.411 kb on - strand, within PP_3405 at 3856.411 kb on - strand, within PP_3405 at 3856.417 kb on + strand, within PP_3405 at 3856.418 kb on - strand, within PP_3405 at 3856.554 kb on + strand, within PP_3405 at 3856.554 kb on + strand, within PP_3405 at 3856.594 kb on - strand, within PP_3405 at 3856.616 kb on + strand, within PP_3405 at 3856.616 kb on + strand, within PP_3405 at 3856.617 kb on - strand, within PP_3405 at 3856.673 kb on - strand, within PP_3405 at 3856.764 kb on - strand at 3856.764 kb on - strand at 3856.834 kb on + strand at 3856.837 kb on - strand at 3856.837 kb on - strand at 3856.904 kb on - strand at 3856.915 kb on + strand at 3856.916 kb on - strand at 3857.148 kb on + strand, within PP_3406 at 3857.149 kb on - strand, within PP_3406 at 3857.154 kb on + strand, within PP_3406 at 3857.154 kb on + strand, within PP_3406 at 3857.155 kb on - strand, within PP_3406 at 3857.155 kb on - strand, within PP_3406 at 3857.155 kb on - strand, within PP_3406 at 3857.160 kb on + strand, within PP_3406 at 3857.161 kb on - strand, within PP_3406 at 3857.190 kb on + strand, within PP_3406 at 3857.190 kb on + strand, within PP_3406 at 3857.190 kb on + strand, within PP_3406 at 3857.190 kb on + strand, within PP_3406 at 3857.191 kb on - strand, within PP_3406 at 3857.205 kb on + strand, within PP_3406 at 3857.205 kb on + strand, within PP_3406 at 3857.205 kb on + strand, within PP_3406 at 3857.205 kb on + strand, within PP_3406 at 3857.205 kb on + strand, within PP_3406 at 3857.206 kb on - strand, within PP_3406 at 3857.206 kb on - strand, within PP_3406 at 3857.341 kb on - strand, within PP_3406 at 3857.341 kb on - strand, within PP_3406 at 3857.513 kb on + strand, within PP_3407 at 3857.513 kb on + strand, within PP_3407 at 3857.549 kb on + strand, within PP_3407 at 3857.549 kb on + strand, within PP_3407 at 3857.550 kb on - strand, within PP_3407 at 3857.652 kb on - strand at 3857.670 kb on + strand at 3857.685 kb on + strand at 3857.686 kb on - strand at 3857.689 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,855,441 + +1.2 3,855,441 + PP_3404 0.16 -0.1 3,855,441 + PP_3404 0.16 -0.5 3,855,442 - PP_3404 0.16 +0.0 3,855,456 + PP_3404 0.18 -0.3 3,855,456 + PP_3404 0.18 +1.7 3,855,457 - PP_3404 0.18 +1.7 3,855,597 + PP_3404 0.34 +0.2 3,855,598 - PP_3404 0.34 -0.4 3,855,598 - PP_3404 0.34 +1.0 3,856,047 + PP_3404 0.86 -0.7 3,856,089 - +0.1 3,856,178 - -0.1 3,856,183 - -0.1 3,856,183 - +0.4 3,856,388 + PP_3405 0.31 -0.9 3,856,388 + PP_3405 0.31 -2.0 3,856,389 - PP_3405 0.31 +0.7 3,856,389 - PP_3405 0.31 -0.4 3,856,389 - PP_3405 0.31 -0.1 3,856,392 + PP_3405 0.32 +0.4 3,856,392 + PP_3405 0.32 +0.7 3,856,392 + PP_3405 0.32 -2.5 3,856,392 + PP_3405 0.32 -2.5 3,856,393 - PP_3405 0.32 -1.0 3,856,393 - PP_3405 0.32 +0.6 3,856,393 - PP_3405 0.32 -0.5 3,856,393 - PP_3405 0.32 -1.8 3,856,393 - PP_3405 0.32 -0.1 3,856,410 + PP_3405 0.34 -1.1 3,856,410 + PP_3405 0.34 -2.0 3,856,411 - PP_3405 0.35 -1.4 3,856,411 - PP_3405 0.35 -0.1 3,856,417 + PP_3405 0.36 +1.8 3,856,418 - PP_3405 0.36 -0.1 3,856,554 + PP_3405 0.57 +0.7 3,856,554 + PP_3405 0.57 -0.0 3,856,594 - PP_3405 0.64 -1.2 3,856,616 + PP_3405 0.67 -0.9 3,856,616 + PP_3405 0.67 +0.2 3,856,617 - PP_3405 0.67 -0.7 3,856,673 - PP_3405 0.76 +1.0 3,856,764 - -0.4 3,856,764 - +0.5 3,856,834 + +0.4 3,856,837 - +2.3 3,856,837 - -0.8 3,856,904 - -1.2 3,856,915 + +0.7 3,856,916 - -0.7 3,857,148 + PP_3406 0.42 -0.3 3,857,149 - PP_3406 0.42 -0.3 3,857,154 + PP_3406 0.43 -0.3 3,857,154 + PP_3406 0.43 +0.0 3,857,155 - PP_3406 0.43 +1.9 3,857,155 - PP_3406 0.43 -1.1 3,857,155 - PP_3406 0.43 -0.5 3,857,160 + PP_3406 0.44 +0.6 3,857,161 - PP_3406 0.45 +0.0 3,857,190 + PP_3406 0.51 +1.9 3,857,190 + PP_3406 0.51 -0.7 3,857,190 + PP_3406 0.51 -1.5 3,857,190 + PP_3406 0.51 -0.2 3,857,191 - PP_3406 0.51 -0.9 3,857,205 + PP_3406 0.54 -0.9 3,857,205 + PP_3406 0.54 +0.3 3,857,205 + PP_3406 0.54 +1.6 3,857,205 + PP_3406 0.54 -0.0 3,857,205 + PP_3406 0.54 -1.5 3,857,206 - PP_3406 0.54 +0.2 3,857,206 - PP_3406 0.54 -0.5 3,857,341 - PP_3406 0.82 -0.3 3,857,341 - PP_3406 0.82 +0.6 3,857,513 + PP_3407 0.39 -1.2 3,857,513 + PP_3407 0.39 +0.6 3,857,549 + PP_3407 0.55 +0.6 3,857,549 + PP_3407 0.55 -0.4 3,857,550 - PP_3407 0.55 +1.0 3,857,652 - +0.8 3,857,670 + -0.7 3,857,685 + -1.3 3,857,686 - +2.0 3,857,689 - +1.4
Or see this region's nucleotide sequence